Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Colitis repairing agent without anti-high cell affinity and application method

A technology for ulcerative colitis and colitis, which is applied in pharmaceutical formulations, organic active ingredients, and medical preparations containing active ingredients, etc., can solve problems such as human health hazards, drug residues, food safety issues, etc. indicators, promoting the development of colon tissue, and reducing the level of intestinal inflammatory factors

Active Publication Date: 2020-09-04
QINGDAO AGRI UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the breeding process, antibiotics are likely to produce drug residues in commercial livestock and poultry products, causing food safety problems and causing harm to human health

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Colitis repairing agent without anti-high cell affinity and application method
  • Colitis repairing agent without anti-high cell affinity and application method
  • Colitis repairing agent without anti-high cell affinity and application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Compounding of the composition

[0020] The dissolution temperature range of duck oil diglyceride in the present invention is 25-40°C; chitosan (MW: 850,000DA; degree of deacetylation: 96.1%) is dissolved in 1% (w / w) at a ratio of 3% (w / w). v / v) Acetic acid saline (the acetic acid solution is dissolved in 0.9% (w / v) physiological saline), that is, the chitosan solution; the duck oil diglyceride and the chitosan solution are in a volume ratio of 1 to 2:1 ( mL / mL); the mixing time of the two is 15-20min.

Embodiment 2

[0021] Example 2: Effects of duck oil diglyceride and chitosan on body weight, colon length and MPO activity of mice with colitis

[0022] The experimental mice were raised in an SPF animal room, kept at a room temperature of 24-25°C, artificial light for 12h / d, and a relative humidity of 40% to 60%. They were fed adaptively for 1 week before the start of the experiment and freely eaten.

[0023] (1) Experimental animals and grouping: The experiment is divided into two stages.

[0024] The first stage: colitis model establishment. Design the control group and the model group, the control group has 5 replicates, and the model group has 20 replicates, each with 10 replicates. All mice eat and drink freely. The mice in the control group drink distilled water freely. In the model group, dextran sodium sulfate (DSS) is dissolved in distilled water to prepare a 5% (w / v) DSS solution instead of drinking water. Rats drink freely for 7 days to induce colitis and establish a model of ulcerat...

Embodiment 3

[0040] Example 3: Synergistic effect of duck oil diglyceride and chitosan on inflammatory factors in mice with ulcerative colitis

[0041] As shown in Table 3, after repair treatment, DDG has a very significant effect on the changes in the concentration of IL-4, IL-17, IL-1β, and IFN-γ (P0.05), but it was lower than other test groups (P<0.05); the IL-6, IL-17, and IL-1β concentrations of group III were lower than those of other test groups. The interaction between DDG and CTS has a significant effect on the changes in the concentration of IL-4, IL-6, and TNF-α (P<0.05). The interaction between DDG and CTS with different additions has a significant effect on the concentration of IL-17, IL-1β and IFN-γ The change effect is extremely significant (P<0.01).

[0042] In summary, the combined repair treatment of duck oil diglyceride and chitosan can reduce the inflammatory response of colitis mice and reduce the release of inflammatory cytokines.

[0043] Table 3 Effect of duck oil diglyce...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a diglyceride preparation containing chitosan. The preparation comprises duck oil diglyceride and chitosan. The invention also provides an ulcerative colitis repairing agent prepared from the diglyceride preparation. A specific preparation method of the ulcerative colitis repairing agent comprises the step of fully mixing and stirring the duck oil diglyceride and the chitosan in a certain volume ratio. 4 mL / kg lactobacillus casei (activity unit: 108 CFU / ml) is fed in 2 h before feeding of the repairing agent. The composition can improve physiological indexes and intestinal flora structure of ulcerative colitis mice, reduce myeloperoxidase activity and reduce expression of inflammatory cytokines, can protect integrity of colonic mucosa and crypt glands, and can effectively repair ulcerative colitis. The ulcerative colitis repairing agent is a novel healthy ulcerative bowel disease repairing agent and clinical treatment scheme with great development value.

Description

Technical field [0001] The invention belongs to the technical field of enteritis treatment and health maintenance preparations, and specifically relates to a non-anti-high cell affinity colitis repair agent. Background technique [0002] Ulcerative colitis is a non-specific colitis. Its clinical manifestations include abdominal pain, bloody stools, etc. The lesions are mainly confined to the mucosa and submucosa. Its pathological changes include ulcer formation, crypt abscess, small vessel inflammation, goblet cell reduction, and Infiltration of various inflammatory cells. The difficulty in the treatment of colitis is that the lesion is located in the back of the intestine. Due to the interference of high gastric acid and various enzymes in the intestine, antibiotic treatment is easy to degrade, the effective concentration is greatly reduced, and it is often difficult to cure and cause a certain degree of resistance. Medicinal properties. At present, the clinical treatment of h...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/722A61K31/23A61P1/00A61P1/04A61K35/747
CPCA61K31/23A61K31/722A61P1/00A61P1/04A61K35/747A61K2300/00Y02A50/30
Inventor 王宝维王茜葛文华张名爱丛红霞徐慧心孔敏凡文磊
Owner QINGDAO AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com