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Nanoscale ultrasonic contrast agent entrapping perfluorocarbon

An ultrasonic contrast agent and perfluorocarbon technology, applied in the field of biomedical materials, can solve the problems of strong rigidity of inorganic nanoparticle materials, poor contrast agent stability, non-therapeutic damage, etc., to improve storage stability and ultrasonic response stability. The effect of stability, high security and strong penetration ability

Pending Publication Date: 2020-09-04
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the shell layer prepared by high molecular polymer is relatively hard and has poor elasticity, and it is easy to form fragments under ultrasonic blasting or natural erosion, resulting in non-therapeutic damage; the contrast agent prepared by surfactant is poor in stability and is not easy to be modified; inorganic nano Granular materials are rigid and require high ultrasonic output

Method used

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  • Nanoscale ultrasonic contrast agent entrapping perfluorocarbon
  • Nanoscale ultrasonic contrast agent entrapping perfluorocarbon
  • Nanoscale ultrasonic contrast agent entrapping perfluorocarbon

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for preparing a nanoscale ultrasound contrast agent loaded with perfluorocarbons includes the following steps:

[0035] (1) Dissolve hydrogenated soybean phospholipids, PEGylated phospholipids and cholesterol in chloroform at a molar ratio, dissolve completely by ultrasonication, and evaporate under reduced pressure at 40°C to remove the organic solvent to obtain a lipid film. Add an appropriate amount of physiological saline as a hydration medium, and hydrate for 30 minutes at 37°C to obtain lipid vesicles.

[0036] (2) Take the liposome prepared in step (1), add perfluorocarbon, and conduct ultrasonic crushing with a probe at 50% strength, and obtain a coarse suspension after ultrasonication for 10 minutes.

[0037] (3) After mixing the coarse suspension prepared in step (2) with the lipid vesicles prepared in step (1), extrude through cellulose membranes of 400nm, 200nm, and 100nm in sequence, and the number of extrusions per layer No less than 13 times, ta...

Embodiment 2

[0040] The storage stability evaluation of the ultrasound contrast agent prepared in Example 1 mainly includes the following steps:

[0041] The prepared contrast agent was placed at 4°C, and samples were taken on the 1st, 2nd, 3rd, 4th, 5th, 6th, and 7th day, and the particle size measurement and Zeta potential evaluation of the samples were carried out.

[0042] See the evaluation results image 3

Embodiment 3

[0044] The in vivo imaging effect evaluation of the ultrasound contrast agent prepared in Example 1 mainly includes the following steps:

[0045] (1) Male SD rats were selected, the abdominal hair was shaved, and barbiturates were injected intraperitoneally for anesthesia. Apply ultrasound coupling agent on the abdomen, and perform hepatic portal venography without contrast agent with ultrasound equipment, and see the appendix for the stored images Figure 4 .

[0046] (2) Dilute the contrast agent described in Example 1 5-10 times with normal saline, inject the contrast agent through the tail vein, and perform hepatic portal venography, and see the appendix for the stored image. Figure 5 .

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Abstract

The invention discloses a nanoscale ultrasonic contrast agent entrapping perfluorocarbon. The prepared nanoscale ultrasonic contrast agent entrapping the low-boiling point perfluorocarbon inside rapidly vaporizes under the action of body temperature and the ultrasonic effect to produce microbubbles; after receiving ultrasonic energy, the microbubbles resonate and scatter ultrasonic signals to achieve the effect of ultrasonic contrast. The ultrasonic contrast agent has a particle size within a nanoscale range, has high penetrating ability, and has better ability of enhancing imaging; moreover,the nanoscale ultrasonic contrast agent is wrapped by multiple layers of phospholipids, so that storage stability is high, in-vivo ultrasonic stability is high, and biological safety is high; a preparation method is simple; the diagnosis and treatment efficiency can be effectively improved; and the nanoscale ultrasonic contrast agent has very high research value and broad application prospects.

Description

technical field [0001] The invention relates to an ultrasonic contrast agent for ultrasonic imaging diagnosis and a preparation method thereof, belonging to the field of biomedical materials. Background technique [0002] Ultrasound imaging is an imaging technique that uses the physical characteristics of ultrasound and the differences in the acoustic properties of human organs and tissues to diagnose diseases. It has received special attention due to its non-invasiveness, low cost, simple operation, and wide range of diagnostic applications. Ultrasound contrast agents are often used in ultrasound diagnosis, which can effectively improve the contrast quality of ultrasound images, significantly improve the ability of ultrasound to distinguish the shape and type of lesion, and enhance the sensitivity and specificity of ultrasound diagnosis. [0003] With the rapid development of ultrasound imaging technology and bio-nanotechnology, nanoscale ultrasound contrast agents are deve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/22A61K47/24A61K47/28
CPCA61K49/221A61K49/227A61K47/24A61K47/28
Inventor 徐郁蕊鲁强兵冯淑君孙磊卢明辉宁兴海
Owner NANJING UNIV
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