G-17, PGI and PGII combined detection device and preparation method thereof

A joint detection and G-17 technology, applied in the direction of measuring devices, biological testing, material inspection products, etc., can solve the problems of insufficient protein adsorption and weak binding force of NC membrane, and achieve reasonable comprehensive judgment, simple operation, Practical effect

Active Publication Date: 2020-09-08
JILIN PROVINCE GERUISITE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The invention provides a G-17, PGI, PGII combined detection device and its preparation method to solve the problems of insufficient NC membrane adsorption protein amount and weak binding force existing in the prior art

Method used

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  • G-17, PGI and PGII combined detection device and preparation method thereof
  • G-17, PGI and PGII combined detection device and preparation method thereof
  • G-17, PGI and PGII combined detection device and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The sample pad 1, the immune colloidal gold glass fiber membrane 2, the immune nitrocellulose membrane 3, and the absorption pad 4 are pasted on the plastic plate 5 respectively, and the two ends of the immune nitrocellulose membrane 3 are respectively connected to the absorption pad 4, the immune colloidal gold glass The fiber membrane 2 is overlapped, and the other end of the immunocolloidal gold glass fiber membrane 2 is overlapped with the sample pad 1; the immunonitrocellulose membrane 3 is provided with a first detection line T1, a second detection line T2, and a third detection line. Line T3 and quality control line C; the first detection line T1 has a high-specificity gastrin 17 antibody on the solid phase; the second detection line T2 has a high-specificity pepsinogen I antibody on the solid phase; There is a highly specific pepsinogen II antibody on the solid phase of the third detection line T3; as figure 1 , 2 As shown, the detection lines T1, T2, and T3 ar...

Embodiment 2

[0067] Prepare colloidal gold particles with a size of 40nm;

[0068] Gold-spraying buffer solution includes: 20mM Tris-HCL solution, 12% sucrose concentration, 3% trehalose concentration, 0.7% BSA concentration, and 8.5 pH;

[0069] Preparation of polyethylene glycol glycerin treatment solution: mixed with polyethylene glycol glycerin and polylysine (SIGMA, 150KD), wherein the concentration of polyethylene glycol glycerin is 0.5%, and polylysine The concentration is 0.5%, filtered through a 0.22μm filter membrane, and set aside;

[0070] The sample pad treatment solution includes: the concentration of Tris-HCL solution is 0.1M, the concentration of bovine serum albumin BSA is 0.7%, the concentration of casein is 0.15%, and the concentration of surfactant is 0.7%;

[0071] All the other are with embodiment 1.

Embodiment 3

[0073] Prepare colloidal gold particles with a size of 60nm;

[0074] Gold-spraying buffer solution includes: 20mM Tris-HCL solution, 20% sucrose concentration, 5% trehalose concentration, 1% BSA concentration, and pH 8.5;

[0075] Preparation of polyethylene glycol glycerin treatment solution: mixed with polyethylene glycol glycerin, polylysine (SIGMA, 150KD) and PEG2000, wherein the concentration of polyethylene glycol glycerin is 0.5%, poly The concentration of lysine is 0.5%, and the concentration of PEG20000 is 0.1%, and it is filtered through a 0.22 μm filter membrane for later use.

[0076] The sample pad treatment liquid includes: the concentration of Tris-HCL solution is 0.1M, the concentration of bovine serum albumin BSA is 1%, the concentration of casein is 0.2%, and the concentration of surfactant is 1%;

[0077] All the other are with embodiment 1.

[0078] Further illustrate the effect of the present invention by experiment below.

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Abstract

The invention relates to a G-17, PGI and PGII combined detection device and a preparation method thereof, and belongs to the field of medical detection equipment. The device is prepared by adhering anitrocellulose membrane with a solid phase containing high-specificity gastrin 17, pepsinogen I, pepsinogen II antibodies and goat anti-mouse IgG polyclonal antibodies, a glass fiber membrane adsorbedwith colloidal gold labeled gastrin 17, pepsinogen I and pepsinogen II antibodies, a sample pad, absorbent paper and other auxiliary materials. The complete release of the immune colloidal gold is ensured; compared with the prior art, the kit has the advantages that the sensitivity of reaction is effectively improved, the dosage of immune colloidal gold can be reduced under the same threshold value, the cost is saved, three gastric functions and gastric cancer risk markers of gastrin 17, pepsinogen I and pepsinogen II in a specimen can be detected at the same time, and the complexity of production operation is not increased. The test paper is high in sensitivity, strong in specificity, simple and convenient to operate, time-saving and strong in practicability.

Description

technical field [0001] The invention relates to the field of medical detection equipment, in particular to a gastrin 17, pepsin I, pepsin II joint detection device and a preparation method thereof, which utilize colloidal gold immunochromatography technology and the principle of double antibody sandwich method to quantitatively detect whole blood, The detection device and preparation method of human gastrin 17 (G-17), pepsinogen I (PGI) and pepsinogen II (PGII) in serum and plasma samples can realize sensitive, specific, and accurate detection of gastric cancer risk markers. Quick check. Background technique [0002] Gastric mucosal lesions are caused by a variety of factors, including drugs, alcohol, abnormal gastric acid secretion, and Helicobacter pylori infection, among which Helicobacter pylori infection is the main factor; after gastric mucosal damage, its function will also change; gastric cancer is a The group of progressive epithelial malignant lesions involving mu...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/573G01N33/558G01N33/58G01N33/574G01N33/543
CPCG01N33/74G01N33/573G01N33/558G01N33/587G01N33/57484G01N33/57446G01N33/54346G01N2333/595G01N2333/96477G01N2800/60G01N33/54366G01N33/54387C07K16/26C07K16/40
Inventor 杨小军李欣
Owner JILIN PROVINCE GERUISITE BIOTECHNOLOGY CO LTD
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