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Microbial inoculum for degrading organophosphorus insecticide as well as preparation method and application of microbial inoculum

A pesticide and organophosphorus technology, applied in the field of bioremediation of pesticide pollution, can solve the problems that degrading strains are difficult to directly use in-situ repairing and affect the degradation activity of degrading strains, and achieve strong biocompatibility, improved tolerance, The effect of reducing the toxic effect

Active Publication Date: 2020-09-11
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complexity of components in actual wastewater and polluted soil, for example, in addition to pesticide components, wastewater is often accompanied by heavy metals, phenols and other pollutants. The existence of these compounds directly affects the degradation activity of degrading strains, making it difficult for degrading strains In-situ remediation directly applied to actual pollution scenarios

Method used

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  • Microbial inoculum for degrading organophosphorus insecticide as well as preparation method and application of microbial inoculum
  • Microbial inoculum for degrading organophosphorus insecticide as well as preparation method and application of microbial inoculum
  • Microbial inoculum for degrading organophosphorus insecticide as well as preparation method and application of microbial inoculum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Preparation of bacterial agents for degrading organophosphorus pesticides

[0048] 1. Pick a ring of strain X1 from the cryopreservation tube, streak it in LB solid medium, and incubate in a constant temperature incubator at 37°C for 24 hours. Pick a single colony and place it in 100mL LB liquid medium, and shake it at 140r / min at 37°C for 12h to obtain a bacterial suspension. The cells were collected by centrifugation at 6000 r / min for 5 min, and washed three times with sterile water to obtain the wet cells of Copperophilus taiwanensis X1. The content of Copperophilus Taiwan X1 in the obtained wet thallus was 10 9 CFU / g.

[0049] 2. Dissolve 1 g of chitosan in 100 mL of 0.05 mol / L dilute hydrochloric acid, add 2 g of diatomaceous earth, stir at room temperature for 1 h, adjust the pH of the solution to neutral with 0.05 mol / L NaOH, collect the precipitate by centrifugation, and dry Grinding and passing through a 200-mesh sieve to obtain a chitosan-diatomit...

Embodiment 2

[0072] Example 2 Degradation of bacterium to chlorpyrifos in pure water and industrial wastewater

[0073] 1g was added to 20mL of pure water containing 100mg / L chlorpyrifos and industrial waste water (taken from the waste water of a pesticide production enterprise in Weifang, Shandong Province, the water quality index is as follows:

[0074] COD: 32500mg / L, BOD 5 : 13000mg / L, Zn 2+ : 84.5mg / L, Pb 2+ : 74.4ug / L, Cd 2+ : 9.3ug / L, NH 3 -N: 55500 mg / L, pH 9.2), cultured in a shaker at 150 r / min at 37°C for 24 hours with constant temperature shaking, and took samples every 2 hours to determine the residual concentration of chlorpyrifos.

[0075] The result is as image 3 As shown, the prepared bacterial agent can maintain good degradation efficiency no matter in pure water or in high-concentration industrial wastewater, and can degrade 50% of 100mg / L chlorpyrifos within 24 hours.

Embodiment 3

[0076] Example 3 Repeated use of bacterial agents

[0077] Add 1 g of the bacterial agent prepared according to the optimal ratio of Example 1 into 20 mL of pure water containing 20 mg / L chlorpyrifos, and culture in a shaker at 150 r / min at 37° C. for 24 hours to sample and determine the residual concentration of chlorpyrifos. Filter the used bacterial agent, wash it 3 times with sterilized water, add it into 20mL pure water containing 20mg / L chlorpyrifos again, measure its degradation effect, repeat several times.

[0078] The result is as Figure 4 As shown, after repeated 2 times, the degradation rate of chlorpyrifos remained above 90.0%; after repeated 6 times, the degradation rate of chlorpyrifos remained stable above 60%.

[0079] The bacterial agent prepared in Example 1 can still maintain degradation activity after being stored at room temperature for 30 days.

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Abstract

The invention provides a microbial inoculum for degrading an organophosphorus insecticide as well as a preparation method and application of the microbial inoculum. The microbial inoculum is preparedby taking an embedding cross-linking product of sodium alginate, chitosan, diatomite and polyvinyl alcohol as a carrier and mixing the embedding cross-linking product with Taiwan cuprophilic bacteriaX1. Materials used for preparing the microbial inoculum are environmentally friendly and high in biocompatibility, and the degradation activity of the bacterial strain X1 on the organophosphorus insecticide can be effectively reserved. The strain is immobilized in the composite material, so that the endurance capacity of the strain to a complex environment is improved, and the toxic action of pollutants in the complex environment on the strain is reduced. The microbial inoculum can efficiently degrade different types of organophosphorus insecticides and can be widely applied to bioremediationof the organophosphorus insecticides in the environment.

Description

technical field [0001] The invention relates to the technical field of bioremediation of pesticide pollution, in particular to a bacterial agent for degrading organophosphorus pesticides and its preparation method and application. Background technique [0002] Organophosphorus insecticides can not only be used for the control of rice leaf moth, gall midge, cotton bollworm, aphid, red spider and other pests on economic crops such as grain, fruit, ornamental plants, cotton, etc., but also can be used in the soil around residential buildings and industrial buildings. Control of termites and subterranean pests. The continued large-scale use of organophosphorus pesticides in agriculture poses a major threat to the environment, food and public health security. Organophosphorus insecticides mainly affect the nervous system of insects by inhibiting the activity of acetylcholinesterase in the body, and then lead to the death of insects. However, for non-target organisms such as mam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/084C12N11/04C02F3/34B09C1/10C12R1/01C02F101/30
CPCC12N11/14C12N11/10C12N11/084C12N11/04C02F3/34B09C1/10C02F2101/306B09C2101/00Y02A50/30
Inventor 花日茂方连城许奕敏吴祥为唐欣昀
Owner ANHUI AGRICULTURAL UNIVERSITY
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