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Production method of lactic acid bacteria plant fermentation lysate

A plant fermentation and production method technology, applied in the direction of pharmaceutical formulations, skin care preparations, medical preparations containing active ingredients, etc., to reduce non-enzymatic browning, eliminate pollution risks, avoid polyphenol oxidative browning and Mela The effect of the German response

Pending Publication Date: 2020-09-29
肽源(广州)生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to ensure the stability of the product, some manufacturers decolorize the lysate of the lactic acid bacteria plant fermentation product with activated carbon. However, the polyphenols that cause browning are often the active ingredients of the plant, so the efficacy of the product is abandoned while decolorizing.

Method used

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  • Production method of lactic acid bacteria plant fermentation lysate
  • Production method of lactic acid bacteria plant fermentation lysate
  • Production method of lactic acid bacteria plant fermentation lysate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A production method of lactic acid bacteria honeysuckle fermentation product lysate, comprising the following steps:

[0029] (1) Powder the dried honeysuckle flowers, then add glucose, nisin and water, and mix uniformly to obtain a plant material liquid; in the plant material liquid, the concentration of honeysuckle powder is 20g / L, and the concentration of glucose is 5g / L L, the concentration of nisin is 0.5g / L;

[0030] (2) Rapidly heat up the plant material liquid to 105°C within 20 minutes and keep it warm for 5 minutes, and then rapidly cool it to 36°C within 20 minutes;

[0031] (3) inoculating Lactobacillus plantarum into the plant material liquid treated in step (2), and anaerobically fermenting at a temperature of 36° C. for 16 hours to obtain a fermented liquid;

[0032] (4) Rapidly heat up the fermentation broth to 75°C within 10 minutes and keep it warm for 10 minutes, then rapidly cool to 30°C within 20 minutes;

[0033] (5) Add 1 g / L of lysozyme to the ...

Embodiment 2

[0038] A production method of lactic acid bacteria Centella asiatica fermentation product lysate, comprising the following steps:

[0039] (1) Centella asiatica dry grass is ground into powder, then add glucose, nisin and water, mix uniformly, obtain plant material liquid; in described plant material liquid, the concentration of centella asiatica powder is 20g / L, the concentration of glucose The concentration of nisin is 5g / L, and the concentration of nisin is 1g / L;

[0040] (2) Rapidly heat up the plant material liquid to 105°C within 20 minutes and keep it warm for 5 minutes, and then rapidly cool it to 36°C within 20 minutes;

[0041] (3) inoculating Lactobacillus plantarum into the plant material liquid treated in step (2), and anaerobically fermenting at a temperature of 36° C. for 24 hours to obtain a fermented liquid;

[0042] (4) Rapidly heat up the fermentation broth to 75°C within 10 minutes and keep it warm for 10 minutes, then rapidly cool to 30°C within 20 minute...

Embodiment 3

[0048] This example is the antioxidant test and high temperature stability test of the lysate of the fermentation product of lactic acid bacteria Lonicerae japonica prepared in Example 1 and the lysate of the fermentation product of lactic acid bacteria Centella asiatica prepared in Example 2.

[0049] Test Methods:

[0050] (1) Antioxidant activity is characterized by DPPH free radical scavenging rate, test steps: (1) Dilute the sample 10 times; (2) According to the industry standard "T-SHRH006-2018 Cosmetics - Free Radical (DPPH) Scavenging Experimental Method" Test the DPPH free radical scavenging rate of the sample diluent.

[0051] (2) The high temperature stability is represented by the absorbance at 475 nm after high temperature treatment. Test steps: (1) Take 15mL sample and add it to a 15mL PET plastic bottle, and store it in a refrigerator at a temperature of 4°C; (2) Take a 100mL sample and put it into a 250mL Erlenmeyer flask, and react in a constant temperature sha...

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Abstract

The invention belongs to the technical field of cosmetic raw materials, and discloses a production method of lactic acid bacteria plant fermentation lysate. The method comprises the following steps ofpreparing plant dry powder or a plant sizing agent, adding glucose, nisin and water, and performing uniform mixing to obtain plant liquid; quickly warming the plant liquid to 100-108 DEG C within 20min, performing heat insulation for 5min, and performing quick cooling within 20min to 35-37 DEG C; inoculating the plant liquid with lactic acid bacteria, and performing anaerobic fermentation at thetemperature of 35-37 DEG C for 16-48h to obtain fermentation liquid; quickly warming the fermentation liquid within 10min to 65-75 DEG C, performing heat insulation for 10-30min, and then performing quick cooling within 20min to 30 DEG C; adding lysozyme of 0.5-2g / L to the fermentation liquid, and performing stirring at the temperature of 30-40 DEG C for 6-18h; and filtering enzymatic hydrolysatewith a filter cloth, and performing centrifugation to remove precipitate to obtain the lactic acid bacteria plant fermentation lysate.

Description

technical field [0001] The invention belongs to the technical field of cosmetic raw materials, in particular to a production method of a lactic acid bacteria plant fermentation lysate. Background technique [0002] Plant fermentation products are a new type of raw materials for skin care products. The active ingredients of plants are extracted and transformed by microbial fermentation technology to achieve the purpose of improving activity, reducing toxicity and producing new efficacy. Lactic acid bacteria are one of the commonly used plant fermentation strains. Various lactic acid bacteria plant fermentation products have appeared on the market, such as Lactobacillus citron peel fermentation product extract, Lactobacillus behenic root fermentation product extract, Lactobacillus pear juice fermentation product filtrate ,wait. The products of lactic acid bacteria fermentation products can be divided into three categories - filtrate, lysate, and extract. The lysate of lactic ...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/9789A61Q19/08
CPCA61K8/99A61K8/9789A61Q19/08A61K2800/85
Inventor 黄亚东梁嘉亮黄志张勇军苏伊婷朱德勇
Owner 肽源(广州)生物科技有限公司