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Microecological preparation for livestock and preparation method of microecological preparation for livestock

A technology of probiotics and additives, applied in the field of microorganisms, can solve the problems of limiting the wide application of probiotics, instability of gastric acid and bile salts, difficulty in meeting treatment needs, etc., to improve feed conversion rate, colonization rate, and The effect of immunity

Inactive Publication Date: 2020-10-13
河北金池牧业科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the probiotics on the market have the following problems: (1) the content of viable bacteria is low, and studies have shown that if the concentration of a bacterium in the cecal content is lower than 107 / g, the bacterium will produce (2) Not resistant to antibiotics; (3) Unstable to high temperature, gastric acid and bile salts, so that only a few strains are still active after entering the intestinal tract, which cannot reach The number of viable bacteria required to function; (4) unstable and short shelf life
Due to these problems in probiotics, it affects its application effect on animals and limits the wide application of probiotics

Method used

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  • Microecological preparation for livestock and preparation method of microecological preparation for livestock

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Experimental program
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Effect test

Embodiment 1

[0029] (1) Weigh 3 grams of soybean meal powder, 4 grams of corn flour, 3 grams of yeast powder, 0.2 grams of potassium dihydrogen phosphate, 0.3 grams of magnesium sulfate heptahydrate, 0.5 grams of calcium carbonate, and 2 grams of sodium acetate to obtain a fermentation additive. Clostridium butyricum is prepared into 4% Clostridium butyricum bacterium liquid with sterile water, measure 4ml of Clostridium butyricum bacterium liquid and inoculate into 3 grams of fermentation additive, statically under the condition of passing carbon dioxide or nitrogen at 37°C Put 20 days and carry out anaerobic fermentation, obtain Clostridium butyricum fermentation product;

[0030] (2) Take Enterococcus faecalis and be mixed with sterile water to be 4% Enterococcus faecalis bacterium liquid, measure 4ml Enterococcus faecalis bacterium liquid and inoculate in 3 grams of fermentation additives, aerobic at 37 ℃ in fermenter Ferment for 10 days to obtain the Enterococcus faecalis fermentation...

Embodiment 2

[0034] (1) Weigh 2 grams of soybean meal powder, 1 gram of corn flour, 1 gram of yeast powder, 0.1 gram of potassium dihydrogen phosphate, 0.1 gram of magnesium sulfate heptahydrate, 0.1 gram of calcium carbonate, and 1 gram of sodium acetate to obtain a fermentation additive. Clostridium butyricum is prepared into a 3% Clostridium butyricum liquid with sterile water, and 3ml of the Clostridium butyricum liquid is inoculated into 1 gram of the fermentation additive, and the clostridium butyricum bacterium is inoculated at 35°C under the condition of carbon dioxide or nitrogen. Put 15 days and carry out anaerobic fermentation, obtain Clostridium butyricum fermentation product;

[0035] (2) Take Enterococcus faecalis and mix it with sterile water to make a 3% Enterococcus faecalis bacterium liquid, measure 3ml Enterococcus faecalis bacterium liquid and inoculate it into 1 gram of fermentation additive, aerobic at 35°C in a fermenter Ferment for 7 days to obtain the Enterococcus ...

Embodiment 3

[0039] (1) Weigh 5 grams of soybean meal powder, 6 grams of corn flour, 5 grams of yeast powder, 0.5 grams of potassium dihydrogen phosphate, 0.5 grams of magnesium sulfate heptahydrate, 1 gram of calcium carbonate, and 3 grams of sodium acetate to obtain a fermentation additive. Clostridium butyricum is prepared into 5% Clostridium butyricum bacteria liquid with sterile water, and 5ml of Clostridium butyricum bacteria liquid is inoculated into 4 grams of fermentation additives, statically at 38°C under the condition of carbon dioxide or nitrogen. Put 30 days and carry out anaerobic fermentation, obtain the clostridium butyricum fermentation product;

[0040] (2) Take Enterococcus faecalis and be mixed with sterile water to be 5% Enterococcus faecalis bacterium liquid, measure 5ml Enterococcus faecalis bacterium liquid and inoculate in 4 grams of fermentation additives, aerobic at 38 ℃ in fermenter Ferment for 15 days to obtain the Enterococcus faecalis fermentation product; ...

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Abstract

The invention discloses a microecological preparation for livestock and a preparation method of the microecological preparation for livestock. The viable count of clostridium butyricum in the microecological preparation for livestock is 2.5-3.5 billions of CFU / g, the viable count of enterococcus faecalis is 9.5-10.5 billions of CFU / g, and the viable count of bacillus subtilis is 4.5-5.5 billions of CFU / g. The preparation method comprises the following steps of taking strains and fermentation additives, performing uniform mixing to obtain mixtures, respectively loading the mixtures into closedcontainers, performing fermentation, performing baking, and performing mixing so as to obtain the microecological preparation for livestock. According to the microecological preparation for livestockdisclosed by the invention, appropriate strains are properly selected for cooperation, so that growth of the livestock can be well promoted, the colonization rate of intestinal tract probiotics can beincreased, the feed conversion rate is increased, probiotic environment can be effectively improved, reproduction of beneficial bacteria can be urged, and reproduction and growth of pathogenic bacteria can be restrained. Through four-year clinical verification, better efficacy of enhancing immunity can be achieved, the immunity can be enhanced through improving stomachs and intestinal tracts of ruminants, and the attack rate of the ruminants can be effectively reduced.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a veterinary microecological preparation and a preparation method thereof. Background technique [0002] Veterinary microecological preparation is a green and safe feed additive, which has multiple functions such as disease prevention, enhancing body immunity, promoting growth, increasing body weight, etc., and has no pollution, no residue, and no drug resistance. Due to the abuse of antibiotics in livestock and poultry breeding, serious food safety incidents have occurred continuously in recent years, which has attracted great attention from governments of various countries. Many countries such as the European Union and the United States have legislated to restrict the use of antibiotics in livestock and poultry breeding. [0003] Veterinary probiotics are probiotics used as feed additives. It can not only promote the establishment of normal microbial flora i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/12A23K10/37A23K10/30A23K20/26A23K20/24A23K20/163A23K20/22A23K10/18C12R1/145C12R1/01C12R1/125
CPCC12N1/20A23K10/12A23K10/37A23K10/30A23K20/26A23K20/24A23K20/163A23K20/22A23K10/18Y02P60/87
Inventor 刘卫东
Owner 河北金池牧业科技发展有限公司
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