Mitochondrion-lysosome migration type membrane potential fluorescent probe CSP
A fluorescent probe, membrane potential technology, applied in fluorescence/phosphorescence, luminescent materials, material analysis by optical means, etc., can solve the problems of limited detection sensitivity, automatic acquisition, practical operation and inconvenience of detection, etc., to achieve real-time Tracking, good reversibility effects
Active Publication Date: 2020-10-16
SHANXI UNIV
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The invention discloses a mitochondrion-lysosome migration type membrane potential fluorescent probe CSP, and belongs to the technical field of mitochondrion membrane potential fluorescent probes. Themitochondrion-lysosome migration type membrane potential fluorescent probe CSP has the near-infrared aggregation-induced fluorescence emission characteristic, and the positive charge structure of themitochondrion-lysosome migration type membrane potential fluorescent probe CSP is combined with the electrostatic interaction of mitochondrion negative membrane potential, so that the probe is aggregated in mitochondria in a targeted manner; the membrane potential is reduced; the probe is released from mitochondria and migrates to lysosome; the probe and the commercially available lysosome specific dye are subjected to co-localization fluorescence imaging, and the characteristic that the co-localization coefficient of the probe and the commercially available lysosome specific dye is increasedalong with reduction of membrane potential is utilized, so that real-time tracking of mitochondrial membrane potential in normal living cells is realized, and visual distinguishing application of dead living cells is realized.
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