Clean production method for extracting tabersonine from African machilus fruits
An African potato fruit and clean production technology, applied in the direction of organic chemistry, can solve the problems of flawed purity, inability to be called a clean process, inability to reflect, etc.
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Embodiment 1
[0044] (1) Reflux extraction: take 100 kg of African potato seed raw material, crush it to a particle size of 1-2 mm, put it into an extraction tank, add a mixed solvent of petroleum ether and ethyl acetate, wherein petroleum ether: ethyl acetate=18 : 1, (V / V), wherein mixed solvent contains 0.8kg castor oil betaine, heating and reflux extracts twice (extracting the consumption of mixed solvent for the first time is 1000L, and the time of reflux is 3 hours; The consumption of solvent is 900L, the time of reflux is 1.5 hours), filter, combine two extracts, obtain mixed solvent extract.
[0045] (2) Silica gel chromatography column adsorption: the mixed solvent extract was passed through a silica gel chromatography column at a flow rate of 0.5 BV / hour (the amount of silica gel used for column chromatography was 30 L, and the height-to-diameter ratio of the silica gel chromatography column was 6:1). The effluent from the silica gel chromatography column was collected and concentr...
Embodiment 2
[0049] (1) Reflux extraction: Take 100kg of African potato seed raw material, crush it to a particle size of 1-2 mm, put it into the extraction tank, add a mixed solvent containing 6# solvent oil and ethyl acetate, 6# solvent oil: acetic acid Ethyl ester=20:1 (V / V), wherein containing 0.6kg castor oil betaine in the mixed solvent, heat reflux extraction twice (the consumption of extracting mixed solvent for the first time is 1000L, and the time of reflux is 2.5 hours; The consumption of extraction mixed solvent is 800L, and the time of reflux is 2 hours), filters, and merges twice extracting solution, obtains mixed solvent extracting solution.
[0050] (2) Silica gel chromatography column adsorption: the mixed solvent extract was passed through a silica gel chromatography column at a flow rate of 0.8 BV / hour (the amount of silica gel used for column chromatography was 25 L, and the height-to-diameter ratio of the silica gel chromatography column was 8:1). The effluent from the...
Embodiment 3
[0054] (1) Reflux extraction: get 100kg of African potato seed raw material, crush it to a particle size of 1 to 2mm, put it into the extraction tank, add a mixed solvent of cyclohexane and ethyl acetate, cyclohexane:ethyl acetate= 15:1, (V / V), wherein the mixed solvent contains 1kg castor oil betaine, heated and refluxed for extraction twice (the amount of mixed solvent for the first extraction is 900L, and the time of reflux is 3 hours; the first extraction mixed The consumption of solvent is 800L, the time of reflux is 2 hours), filter, combine two extracts, obtain mixed solvent extract.
[0055] (2) Silica gel chromatography column adsorption: the mixed solvent extract was passed through the silica gel chromatography column at a flow rate of 0.6BV / hour, the amount of silica gel used for column chromatography was 28L, and the height-to-diameter ratio of the silica gel chromatography column was 7:1. The effluent from the silica gel chromatography column was collected and con...
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