Rhodotorula diobovate strain capable of producing carotenoid and application thereof

A carotene and red yeast technology, applied in the field of marine microorganisms, can solve the problems of high cost, low content, complex carotenoid extraction process, etc.

Active Publication Date: 2020-10-23
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although carotenoids extracted from nature are highly safe, the extraction process of carotenoids is complicated and costly due to its low content

Method used

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  • Rhodotorula diobovate strain capable of producing carotenoid and application thereof
  • Rhodotorula diobovate strain capable of producing carotenoid and application thereof
  • Rhodotorula diobovate strain capable of producing carotenoid and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Screening of marine rhodotorula producing carotenoids

[0015] 1. Preparation of culture medium

[0016] 1) The selection medium for Rhodotorula marinae is WL nutrient agar medium: 5g / L yeast powder, 5g / L peptone, 50g / L glucose, 0.55g / L potassium dihydrogen phosphate, 0.425g / L potassium chloride, 0.125g / L calcium chloride, 0.125g / L magnesium sulfate, 0.0025g / L ferric chloride, 0.0025g / L manganese sulfate, 0.022g / L bromocresol green, 20g / L agar powder, 0.1g / L chloramphenicol , pH6.5. The above-mentioned culture medium was prepared with old sea water, and after autoclaving at 115°C for 20 minutes, it was poured into a plate for later use, and bromocresol green and chloramphenicol were added separately when the sterilization medium was cooled to 65°C.

[0017] 2) Carotenoid-producing screening medium: 20g / L glucose, 2g / L ammonium sulfate, 1g / L potassium dihydrogen phosphate, 0.5g / L magnesium sulfate, 1g / L calcium chloride, 5g / L yeast powder, Prepared with ol...

Embodiment 2

[0024] Embodiment 2: the genus identification of marine rhodotorula ZQ38

[0025] Morphological features: such as figure 1 As shown, the colonies of Rhodotorula marina ZQ38 grown on WL nutrient agar medium are medium-sized, round or oval, with smooth and moist surface, raised in the middle, neat edges of colonies, and uniform color, showing brick red; figure 2 As shown, the morphology of the bacterial cells observed under a microscope is round or oval, and it is polygonal budding.

[0026] Physiological and biochemical identification was carried out by an automatic microbial analyzer. The results of the test and identification are shown in Table 2. It was in line with the physiological and biochemical characteristics of Rhodotorula, and it was preliminarily determined to be Rhodotorula double obovata.

[0027] Table 2 Physiological and biochemical identification results of Rhodotorula marinum ZQ38

[0028]

[0029] Sequence analysis of the 26S rDNA D1 / D2 region of Rhodo...

Embodiment 3

[0032] Example 3: Production of carotenoids by fermentation of Rhodotorula biobversus ZQ38

[0033] 1) Activation of strains: Pick one ring of Rhodotorula biobovata ZQ38 strains preserved on the slope, inoculate into a 50mL / 250mL Erlenmeyer flask, and activate and cultivate at 28°C and 200r / min for 48h. Activation medium used: 20g / L glucose, 20g / L peptone, 10g / L yeast powder, prepared with aged sea water, natural pH, sterilized at 115°C for 20min.

[0034] 2) Fermentation to produce carotenoids

[0035] Using optimal fermentation medium 1: 15g / L sucrose, 2g / L ammonium sulfate, 1g / L potassium dihydrogen phosphate, 0.5g / L magnesium sulfate, 1g / L calcium chloride, 5g / L yeast powder, and aged seawater. The pH is natural, the filling volume is 100mL / 500mL, and it is sterilized at 121°C for 20min. Inoculate the activated strain with 6% (v / v) inoculum, ferment and cultivate for 3 days in a constant temperature shaker with a culture temperature of 28°C and a rotation speed of 200r / m...

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Abstract

The invention discloses a rhodotorula diobovate strain capable of producing carotenoid and application thereof. The rhodotorula diobovate strain is preserved in China General Microbiological Culture Collection Center on June 22, 2020., with an accession number being CGMCC No. 20126. The rhodotorula diobovate ZQ38 provided by the invention belongs to a marine microorganism source, is an excellent production strain of natural carotenoid, does not produce toxin, is a non-recombinant bacterium, has high safety, and meets food safety requirements; and the strain has the advantages of simple culturerequirements, easily-controllable fermentation conditions, low nutritional requirements and high carotenoid yield, and thus has wide application prospects in the aspect of biosynthesis of carotenoid.

Description

technical field [0001] The invention belongs to the technical field of marine microorganisms, and in particular relates to a rhodotorula biobvalveta strain producing carotenoids isolated from the marine environment. Background technique [0002] Carotenoids are a class of isopentenoid polymers containing 40 carbons. The color of carotenoids changes due to the number of conjugated double bonds. The more the number of conjugated double bonds, the more red the color will be. As an antioxidant, carotenoids have been recognized all over the world. They are excellent food additives and nutritional supplements, and are widely used in food, cosmetics, medicine, feed and other industries. [0003] The production of carotenoids mainly includes chemical synthesis, biosynthesis and natural extraction. The food safety of carotenoids prepared by chemical synthesis has attracted more and more attention from the society due to problems such as solvent residues, thus forcing people to seek ...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P23/00C12R1/645
CPCC12P23/00C12N1/145C12R2001/645
Inventor 程仕伟仉倩门一波董全行李雯刘金艳曾世婕
Owner LUDONG UNIVERSITY
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