Bacillus velezensis and application thereof to relieving continuous cropping obstacles of apples

A technology of Bacillus velesi and Bacillus, applied in the field of agricultural microorganisms, can solve the problems of few Bacillus and continuous cropping obstacles of apples, and achieve the effect of promoting plant growth and reducing continuous cropping obstacles of apple trees.

Active Publication Date: 2020-11-03
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few studies on the prevention and tre...

Method used

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  • Bacillus velezensis and application thereof to relieving continuous cropping obstacles of apples
  • Bacillus velezensis and application thereof to relieving continuous cropping obstacles of apples
  • Bacillus velezensis and application thereof to relieving continuous cropping obstacles of apples

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1: Isolation and identification of bacterial strains

[0052] 1. Isolation and purification of strains:

[0053] The rhizosphere bacteria were separated by the improved dilution coating plate method, and fresh soil samples were taken from the rhizosphere soil of three continuous cropping apple orchards in Xicheng Town (XC), Wantou Village (WT) and Fengmaozhai (FM) in Yantai City. 3 ~ 4mm sieve to remove impurities in the soil. Weigh 5 g of rhizosphere soil, add it to a triangular flask (with glass beads) filled with 45 mL of sterile water, vibrate at 180 rpm for 30 min, let it stand for 5 min, and serially dilute to 1×10 with sterile water. -4 100 μL of the supernatant of the gradient dilution was spread on the LB plate, repeated three times, and cultured upside down in a constant temperature incubator at 28°C. After a single colony grows in the plate, pick a single colony of different shapes and streak on a new LB plate for purification. At the same time, th...

Embodiment 2

[0078] Example 2: Functional identification of Bacillus velezensis XC1

[0079] 1. Antibacterial effect:

[0080] The antibacterial effect of XC1 was evaluated by the filter paper method, and the XC1 strain was inoculated in LB liquid medium, 37°C, 180r min -1 Cultivate on a shaker for 24 hours, use a 5mm diameter puncher to punch out the bacterial mass at the edge of the pathogenic bacteria, inoculate it into the center of the PDA plate, put sterile filter paper at the 4 docking points 2cm away from the pathogenic bacterial mass, and inject 10 μL of XC1 bacterial solution, After 2 hours of pre-diffusion, culture at 28°C. Use the pathogenic bacteria not inoculated with the bacteria solution as a control, and observe at any time. After the control is full of the plate, use a vernier caliper to measure the diameter of the fungal colony using the cross method, and the diameter represents the antibacterial rate. Repeat the above operation 3 times.

[0081] Bacterial inhibition r...

Embodiment 3

[0092] Embodiment 3: pot test

[0093] 1. Experimental design:

[0094] The pot experiment was carried out at the National Apple Engineering Experimental Center and the Apple Replanting and Microbiology Laboratory of the South Campus of Shandong Agricultural University from March to October 2019. The sweet tea seeds are stratified at 4°C for about 30 days, and after the seeds turn white, they are sown in a cultivation pot equipped with a seedling substrate for seedling cultivation. When the seedlings grow to 6 true leaves, select plants with consistent growth and no pests and diseases, and transplant them on May 1 into clay pots with 6.5 kg of different treatment soil (the upper diameter is 25 cm, the lower diameter is 17 cm, and the height is 18 cm). , Sampling in mid-September to determine relevant experimental indicators.

[0095] The potting test soil was taken from a 32-year-old apple orchard in Qingwan, Manzhuangtan, Tai'an City, Shandong Province, from an area 80.00 c...

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Abstract

The invention discloses bacillus velezensis and an application thereof to relieving continuous cropping obstacles of apples. The bacillus velezensis is preserved in China General Microbiological Culture Collection Center on June 10, 2020, and the biological preservation number of the bacillus velezensis is CGMCC NO. 20057. The bacillus velezensis XC1 provided by the invention is a novel biocontrolbacterium having great development potential and has a relatively strong inhibition effect on f. oxysporum, f. moniliforme, f. proliferatum and f. solani, and the inhibition rate of the bacillus velezensis XC1 on the f. oxysporum is the highest and reaches 79.83%. The strain can achieve high yield of antagonistic enzymes such as protease and cellulase; the content of phenolic acid substances suchas phlorizin, phloretin, ferulic acid and cinnamic acid in the soil is reduced; apple tree plant growth is promoted; the method has an excellent effect in relieving continuous cropping obstacles of apple trees, and provides a theoretical basis for biological prevention and treatment of the continuous cropping obstacles of the apple trees.

Description

technical field [0001] The invention relates to the technical field of agricultural microbes, in particular to a strain of bacillus velei and its application in alleviating obstacles to continuous cropping of apples. Background technique [0002] With the renewal of varieties and the increase of planting area, there are large-scale old orchards in my country that need to be renovated, and the continuous cropping obstacles faced by old orchards are very common. Wang Gongshuai (2018) and Wang Xiaobao et al. (2018) showed that harmful fungi of the genus Fusarium are the main pathogenic bacteria that cause apple continuous cropping obstacles in the Bohai Bay and Northwest Loess Plateau regions; Xu Wenfeng (2011) and Liu Zhi (2013) respectively A large number of Fusarium oxysporum (F.oxysporum), F. moniliforme (F. moniliforme), Fusarium solani (F. proliferatum, and verified that it has strong pathogenicity to Pingyi sweet tea seedlings. Wang Gongshuai (2018) found that these fou...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/42C12N9/54A01B79/00B09C1/10A01N63/22A01P21/00C12R1/07
CPCA01B79/00A01N63/22B09C1/10B09C2101/00C12N9/2437C12N9/54C12N1/205C12R2001/07
Inventor 尹承苗闫助冰毛志泉陈学森沈向胡艳丽
Owner SHANDONG AGRICULTURAL UNIVERSITY
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