Application of saccharomyces cerevisiae in postharvest disease control, storage and preservation of baby Chinese cabbage

A technology for Saccharomyces cerevisiae and baby cabbage, which is applied in the field of postharvest disease control, storage and preservation, and Saccharomyces cerevisiae to achieve the effects of controlling quality decline, slowing the increase in organic acid content and reducing potential harm

Active Publication Date: 2020-11-06
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no yeast for the prevention and treatment of postharvest black spot disease of baby cabbage

Method used

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  • Application of saccharomyces cerevisiae in postharvest disease control, storage and preservation of baby Chinese cabbage
  • Application of saccharomyces cerevisiae in postharvest disease control, storage and preservation of baby Chinese cabbage
  • Application of saccharomyces cerevisiae in postharvest disease control, storage and preservation of baby Chinese cabbage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Inhibitory effects of different yeasts on controlling baby cabbage postharvest diseases in vitro;

[0036] 1. Test plan

[0037] 1. Preparation of Yeast Suspension

[0038] Put 50mL NYDB medium in a 250mL Erlenmeyer flask, suck 1mL of activated Saccharomyces cerevisiae Y-912 with a pipette gun, and culture it at 28°C and 180rpm for 24 hours to obtain a yeast culture solution, centrifuge it at 7000r / min for 15min, The cells were collected, washed twice with sterile saline, and configured to a size of 1.0×10 8 cells / mL of yeast suspension.

[0039] 2. Preparation of Mold Spore Suspension

[0040] Scrape the Alternaria tenoides spores on the PDA medium, blow and beat repeatedly in sterile saline, shake for 10 minutes, and adjust the concentration to 5.0×10 4 spores / mL of spore suspension.

[0041] 3. The in vitro inhibitory effect of different yeasts on Alternaria adenocarina

[0042] Pour 30mL of PDA medium into a sterile 9cm petri dish, cool and solidify, and punch...

Embodiment 2

[0046] Saccharomyces cerevisiae Y-912 is used for the purposes of black spot control after harvesting Chinese baby cabbage;

[0047] 1. Test plan

[0048] First of all, the Saccharomyces cerevisiae Y-912 of the present invention is used to control postharvest black spot of baby cabbage. The specific steps are: select fresh baby cabbage, remove the outer layer, and select a product with the same size and color, no mechanical damage on the surface, and no damage by diseases and insect pests. Soak the whole leaf with 0.02% sodium hypochlorite solution for 1 min, then rinse it with clean water, and put it in a sterile plastic basket to dry.

[0049] Carry out different treatments at the leaves and petioles of the vegetable leaves: the leaf part of the vegetable leaves is evenly sprayed with 1.0×10 8 cells / mL of the yeast suspension, after 2h, spray the Alternaria tennatus spore suspension (concentration is 1×10 6 spores / mL); the control group was treated with sterile saline inst...

Embodiment 3

[0065] The use of Saccharomyces cerevisiae Y-912 in the storage and preservation of baby cabbage, that is, the effect on postharvest natural decay and quality (Vc, titratable acid, weight loss rate);

[0066] Put the baby cabbage leaves with the same size, no mechanical damage on the surface and no damage from diseases and insect pests in a sterilized plastic basket, and put 1.0×10 8 The yeast suspension of cells / mL is sprayed on the surface of baby cabbage leaves to make it evenly covered; after drying, seal it, store it in a constant temperature incubator (RH 90%, 20°C) and count the baby cabbage rot rate after a certain period of time and disease index, and measured the Vc and titratable acid content of leaves and petioles, as well as the weight loss rate of vegetable leaves. Each treatment was repeated 5 times with 12 vegetable leaves each time.

[0067] The formula for calculating the decay rate is as follows:

[0068] Rot rate = number of rotten leaves / total number of ...

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Abstract

The invention belongs to the field of biological prevention and control of postharvest diseases of fruits and vegetables, and relates to application of saccharomyces cerevisiae in postharvest diseasecontrol, storage and preservation of baby Chinese cabbage. The application of controlling the postharvest diseases comprises the steps of selecting vegetable leaves of the baby Chinese cabbage, airingwith disinfection, washing with clear water, putting into a sterile plastic basket, airing, uniformly spraying a saccharomyces suspension on the surfaces of the vegetable leaves, standing, spraying an alternaria tenuissima spore suspension, naturally airing, and sealing with a preservative film, thereby controlling the black spot disease of the baby Chinese cabbages; the application for storage and preservation comprises the following steps of putting the baby Chinese cabbages in the disinfected plastic basket, uniformly spraying saccharomyces suspension on the surfaces of the baby Chinese cabbages, naturally airing the baby Chinese cabbages, sealing the baby Chinese cabbages with a preservative film, and storing the baby Chinese cabbages at the temperature of 20 DEG C to realize prevention and treatment of diseases of the postharvest disease control and storage and preservation of the baby Chinese cabbages. The application is safe and environment-friendly, not only can effectively control the postharvest black spot disease, but also can slow down the reduction of the quality of the baby Chinese cabbage to a certain extent, the postharvest black spot disease can be controlled through the application which can replace chemical bactericide, and the potential application value is achieved.

Description

technical field [0001] The invention belongs to the field of biological control of postharvest diseases of fruits and vegetables, and in particular relates to the use of Saccharomyces cerevisiae for postharvest disease control and storage and preservation of baby vegetables. Background technique [0002] Baby cabbage is a small pocket cabbage, which belongs to the Brassica cabbage subspecies and is a semi-cold-resistant vegetable. Baby cabbage is crisp and tender, rich in nutrition, high in quality and low in price, and high in edible rate. It is a popular vegetable variety with extremely high market value. Baby cabbage is rich in B vitamins, vitamin C, carotene, folic acid, and trace elements such as calcium, phosphorus, and iron, and has a high chlorophyll content. Baby cabbage helps gastrointestinal motility, promotes defecation, quenches thirst and diuresis. Baby cabbage can inhibit the absorption of nitrite, reduce the occurrence of breast cancer, and have the functio...

Claims

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Application Information

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IPC IPC(8): A23B7/155C12N1/18C12R1/865
CPCA23B7/155C12N1/18A23V2002/00A23V2200/10A23V2250/762
Inventor 张红印张晓云孙露王子洋李鹏霞张雷刚赵利娜
Owner JIANGSU UNIV
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