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Application of plant protein zymolyte as polypeptide surfactant

A technology of protease hydrolyzate and surfactant, which is applied in the field of light chemical industry to achieve high safety, biocompatibility and strong emulsification effect

Inactive Publication Date: 2020-11-10
BEIJING INSTITUTE OF TECHNOLOGYGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, plant protein hydrolyzate is a short-chain peptide segment formed by proteolysis of plant protein, which is mainly used for taste, nutrition and functional health care, and there is no report for emulsification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Measure 100mL of water and heat it to 50°C in a 250mL beaker, add 10g of soybean protein isolate (Shandong Jiahua Oil Co., Ltd.) to prepare a feed liquid;

[0038] (2) Regulating the pH of the feed liquid with a concentration of 2M NaOH solution is 7.5, stirring until the soybean protein isolate reaches a dissolved state;

[0039] (3) Add 0.3 g of alkaline protease (Novozymes) to obtain a reaction solution;

[0040] (4) Stir the reaction solution at 60 rpm to 200 rpm until fully mixed, and enzymatically hydrolyze at 50°C for 3 hours;

[0041] (5) Heating to 100° C. and maintaining for 5 minutes to inactivate enzyme activity to obtain soybean polypeptide enzymatic hydrolysis solution.

[0042] The soybean polypeptide enzymatic hydrolyzate prepared in this embodiment was detected, and the results were as follows:

[0043] The degree of hydrolysis of the soybean polypeptide hydrolyzate was determined to be 17%; the soybean polypeptide hydrolyzate was diluted with wat...

Embodiment 2

[0045] (1) Take 100mL of distilled water and heat it to 50°C in a 250mL beaker, add 5g of soybean protein isolate (Shandong Yuwang Food Co., Ltd.) to prepare a feed liquid;

[0046] (2) Regulate the pH of the feed liquid with 2M NaOH solution to be 7.0, and stir until the soybean protein isolate reaches a dissolved state;

[0047] (3) Add papain (Nanning Pangbo Biological Engineering Co., Ltd.) 0.05g to obtain the reaction solution;

[0048] (4) Stir the reaction solution at 60 rpm to 200 rpm until fully mixed, and enzymatically hydrolyze at 50°C for 6 hours;

[0049] (5) Heating to 100° C. and maintaining for 5 minutes to inactivate enzyme activity to obtain soybean polypeptide enzymatic hydrolysis solution.

[0050] The soybean polypeptide enzymatic hydrolyzate prepared in this embodiment was detected, and the results were as follows:

[0051] The degree of hydrolysis of the soybean polypeptide hydrolyzate was determined to be 14%; the soybean polypeptide hydrolyzate was dil...

Embodiment 3

[0053] (1) Measure 100mL of distilled water into a 250mL beaker, heat to 55°C, add 10g of peanut protein isolate (Henan Liangjian Technology Co., Ltd.) to prepare a feed solution;

[0054] (2) Use a hydrochloric acid (HCl) solution with a concentration of 2M to adjust the pH of the feed solution to 6.0, and stir until the peanut protein isolate reaches a dissolved state;

[0055] (3) Add 0.05 g of Protamex protease (novozymes) to obtain a reaction solution;

[0056] (4) Stir the reaction solution at 60 rpm to 200 rpm until fully mixed, and enzymatically hydrolyze at 55°C for 4 hours;

[0057] (5) Heating to 100° C. and maintaining for 5 minutes to inactivate enzyme activity to obtain peanut polypeptide enzymatic hydrolyzate.

[0058] The peanut polypeptide enzymatic hydrolyzate prepared in this example was detected, and the results were as follows:

[0059] The degree of hydrolysis of the peanut polypeptide hydrolyzate was determined to be 18%; the peanut polypeptide hydroly...

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Abstract

The invention relates to an application of a plant protein zymolyte as a polypeptide surfactant, and belongs to the technical field of light chemical engineering. According to the application, the plant protein zymolyte with the surface tension ranging from 25 mN / m to 45 mN / m when the mass fraction of protein ranges from 0.4% to 1% in a solution or suspension state is used as the polypeptide surfactant, and the polypeptide surfactant can be used as an emulsifying agent for food seasonings, protein beverages, meat products or cosmetics. Compared with the emulsifying effect of the protein, the emulsifying effect of the polypeptide surfactant is stronger. Polypeptide has small molecular weight and is easily absorbed by the skin, hair and intestinal tract of the human body, so that the polypeptide has a nutritional effect. The polypeptide surfactant also has the effects of moisturizing the skin, resisting oxidation, reducing blood pressure, reducing blood fat, resisting bacteria and the like, has extremely high safety and biocompatibility, and is a multifunctional biological surfactant. The plant protein zymolyte can be applied to cosmetics, medicines, foods and other products in direct contact with the human body.

Description

technical field [0001] The invention relates to the application of a plant protein hydrolyzate as a polypeptide surfactant, belonging to the technical field of light chemical industry. Background technique [0002] Biosurfactants are natural surfactants produced by microorganisms, animals, and plants. Compared with chemically synthesized surfactants, the raw materials of biosurfactants are renewable biomass resources, which have the advantages of easy degradation, high biocompatibility and high safety, and are especially suitable for products in direct contact with the human body, such as cosmetics, pharmaceuticals and food etc. [0003] The hydrophobic ends of biosurfactants generally have long fatty acid chains, and the hydrophilic ends are generally sugars, cyclic peptides, amino acids, phosphate carboxylic acids or alcohols, and the molecular weight is generally 500Da to 1500Da. Biosurfactants can be divided into glycolipids, phospholipids, fatty acids, lipopeptides an...

Claims

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Application Information

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IPC IPC(8): A23L29/10A23L33/00A61K8/64A61Q19/00A61Q19/08A61Q17/00A61K38/01A61P9/12A61P3/06
CPCA23L29/10A23L33/00A61K8/645A61Q19/00A61Q19/08A61Q17/005A61K38/011A61P9/12A61P3/06
Inventor 周晓宏廖永红
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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