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Preparation method and application of antler ethanol extract

A technology of extract and velvet alcohol, applied in the field of preparation of velvet alcohol extract

Pending Publication Date: 2020-11-27
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although drugs such as levodopa and dopamine agonists have temporarily relieved patients' symptoms, so far there are no effective therapies or drugs that can prevent or delay the neurodegenerative process of PD

Method used

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  • Preparation method and application of antler ethanol extract
  • Preparation method and application of antler ethanol extract
  • Preparation method and application of antler ethanol extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1. The preparation method of the velvet alcohol extract which has the effect of preventing and / or treating Parkinson's disease.

[0043] 1) Mix absolute ethanol with velvet antler freeze-dried powder and extract to obtain an extract; the specific method is as follows:

[0044] The fresh sika deer Ergang antler purchased from Zuojia Specialty Research Institute of Jilin Province was cut into 2-3mm thick slices with a slicer, and placed at -80°C and 0.04bar for 48 hours of freeze-drying, and then The freeze-dried velvet slices were crushed into velvet freeze-dried powder with a cryogenic grinder; 10 g of velvet freeze-dried powder was mixed with 50 mL of absolute ethanol, and the obtained mixture was refluxed at a water bath temperature of 80° C. for 1 hour to obtain an extract.

[0045] 2) Filter the extract obtained in step 1) after cooling, collect the supernatant, and leave the residue for subsequent use;

[0046] 3) Concentrate the supernatant obtained in st...

Embodiment 2

[0047] Example 2, rough separation of velvet antler alcohol extract and identification of crude components

[0048] Mix the antler alcohol extract and petroleum ether according to the ratio of solid to liquid 23.91g:1000mL, let it stand for 1 day, filter it, wash and precipitate with methanol to obtain the antler inorganic salt component, the content of which is 6.4%; the remaining petroleum ether solution Partially through medium pressure preparative chromatography, with dichloromethane:methanol=50:50 (v:v) and dichloromethane:methanol=0:100 (v:v) as eluent elution to obtain MEs-1, MEs respectively -2 and MEs-3 components, the contents were 46.2%, 16.7% and 14.9%, the separation process is as follows figure 1 shown.

[0049] MEs-1 and MEs-2 were determined by gas chromatography for free fatty acid content, MEs-1 contained 3.96% fatty acid, MEs-2 contained 2.34% fatty acid, among which pentadecanoic acid, palmitic acid, stearic acid, oleic acid, peanut four Alkenic acid cont...

Embodiment 3

[0055] Embodiment 3, velvet antler alcohol extract chemical composition analysis

[0056] Polyacrylamide gel electrophoresis (SDS-PAGE) was used to detect whether there was protein in the velvet alcohol extract: the loading amount of velvet alcohol was 50 μg, and the loading volume was 20 μL. Using 5% stacking gel and 12% separating gel, SDS-PAGE electrophoresis was carried out at 100V for 90min. After electrophoresis, protein bands were observed after staining with Coomassie brilliant blue. Such as figure 2 As shown, there is no protein in the velvet antler alcoholic extract obtained through the above-mentioned preparation method.

[0057]Instrument Brukerav-300 spectrometer (300MHz 1 H, 75MHz 13 C), the compounds in velvet antler alcohol extract DAE-100 were identified by nuclear magnetic resonance (NMR). 1 The solvent of H-NMR is DMSO-D6, 13 The solvent for C-NMR is MEOD. 1 HNMR and 13 C NMR evaluation of the properties of velvet antler alcoholic extract. Chemical...

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Abstract

The invention belongs to the technical field of medicines, and provides a preparation method and application of antler ethanol extract. In order to research the curative effect of the antler ethanol extract on neurodegeneration and neuroinflammatory response of a Parkinson's disease (PD) mouse induced by MPTP, the antler ethanol extract is used for carrying out treatment on the PD model mouse, andexperiments prove that the antler ethanol extract can improve the behavioral disorder of the PD model mouse and take the effects of inhibiting phosphorylated Akt, p65 and p38 signal pathways in striatum, reducing the activation of astrocytes and protecting substantia nigra (SN) and striatal neurons from being damaged. Therefore, the antler ethanol extract disclosed by the invention has a good treatment effect on a PD animal model, has a wide application prospect in the aspects of treating the PD and other inflammation and oxidative stress related neuron diseases, and provides an experimentalbasis for the research and development of new drugs for treating the PD.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a preparation method and application of antler alcohol extract. Background technique [0002] Parkinson's disease (PD) is a common neurodegenerative disorder that affects nearly 10 million people worldwide. PD is characterized by the progressive loss of dopaminergic neurons and the formation of Lewy bodies in the brain. It is mainly manifested as movement disorders such as slow telekinesia, muscle rigidity, tremor, and abnormal posture and gait. It is often accompanied by hyposmia, sleep disorders, etc. Non-motor symptoms, most patients are sporadic cases, and less than 10% have family history. The exact etiology of Parkinson's disease is still unclear. Genetic factors, environmental factors, aging, oxidative stress, etc. may all be involved in the degeneration and death process of PD dopaminergic neurons. Although drugs such as levodopa and dopamine agonists have temporarily re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/32A61P25/16
CPCA61K35/32A61P25/16
Inventor 彭英华刘莹常树卓杨敏金沙李宏源
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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