Purification process of acetal phospholipid

A plasmalogen and process technology, which is applied in the field of plasmalogen purification process, can solve the problems of qualitative and quantitative difficulties of plasmalogen, and achieve the effect of increasing extraction capacity, increasing recovery rate, and improving recovery rate

Inactive Publication Date: 2020-11-27
ZHEJIANG GONGSHANG UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complex chemical structure of plasmalogen, the determination and characterization of plasmalogen is still a challenge, that is, the qualitative and quantitative analysis of plasmalogen is still difficult.
Currently, there are only a few reports on methods for the qualitative and quantitative analysis of plasmalogen by mass spectrometry coupled with liquid chromatography (LC), gas chromatography (GC) and capillary electrophoresis (CE).

Method used

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  • Purification process of acetal phospholipid
  • Purification process of acetal phospholipid
  • Purification process of acetal phospholipid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] Example 1. A kind of purification process of plasmalogen, comprises the following steps,

[0099] a1. Dissolve 10mmol urea and 2.86mmol of CPB (i.e. cetylpyridinium bromide) in every 30mL of water to obtain the A1 product.

[0100] a2. Add 30mL of cyclohexane and 0.94mL of 2-propanol to the A1 product to obtain the A2 product.

[0101] a3. Add 3 mL of tetraethyl orthosilicate to product A2 to obtain product A3.

[0102] a4. Stir product A3 for 30 minutes to obtain product A4.

[0103] A5 and A4 products were heated to 70°C and stirred for 20 hours to obtain A5 products.

[0104] A6 and A5 products were centrifuged for 10 minutes to obtain A6 products.

[0105] A7, A6 product are washed with acetone, deionized water and ethanol successively to obtain A7 product,

[0106] a8, A7 products were vacuum-dried for 12 hours to obtain A8 products,

[0107] A9 and A8 products were calcined at 450-550°C for 4 hours to obtain KCC-1, namely A9 products;

[0108] Dissolve 2.2g...

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Abstract

The invention discloses a purification process of acetal phospholipid, comprising the following steps of: a, synthesizing TiO2 / KCC-1 core-shell microspheres to obtain a product A; b, performing crudeextraction on clams to obtain a product B; c, preparing an SPE micro-column by using the product A and the product B to obtain a product C; and d, purifying the product B by using the product C to obtain acetal phospholipid. In the step a, urea and CPB in water are dissolved in water; adding cyclohexane and 2-propanol into the product A1 to obtain a product A2, adding tetraethoxysilane into the product A2 to obtain a product A3, stirring the product A3 to obtain a product A4, stirring the product A4 to obtain a product A5, centrifuging the product A5 to obtain a product A6, washing the productA6 to obtain a product A7, drying the product A7 to obtain a product A8, and calcining the product A8 to obtain a product A9; dissolving tetrabutyl titanate in ethanol to obtain a product A10, and oscillating the product A10 to obtain a product A11; and adding the product A9 into the product A11 to obtain a product A12, and maintaining the product A12 to obtain a product A13. The purification process has the advantages that acetal phospholipid rich in monounsaturated fatty acid can be selectively purified from clams, and the recovery rate is high.

Description

technical field [0001] The invention relates to a purification process of clam plasmalogen. Background technique [0002] Plasmalogens are unique membrane glycerophospholipids containing fatty alcohols with ene-ether linkages, predominantly monounsaturated or saturated fatty acids at the sn-1 position, and linked polyunsaturated fatty acids (PUFAs) at the sn-2 position , usually eicosapentaenoic acid (EPA, C20:5n-3), docosahexaenoic acid (DHA, C22:6n-3), etc., and the sn-3 position is a polar head. The main types of plasmalogen are plasmalogen phosphatidylethanolamine (plasPE), plasmalogen phosphatidylcholine (plasPC) and plasmalogen phosphatidylserine (plasPS), widely present in human cells and tissues, especially in the brain, lung, Kidney and skeletal muscle. Plasmalogens have many important functions, including participation in cholesterol efflux, signal transduction and membrane fusion, protection of cells from the activity of endogenous antioxidants, and acting as a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/02B01J13/06G01N30/06B01J20/28B01J20/10B01D15/08C07F9/10
CPCB01J13/02B01J13/06G01N30/06B01J20/28021B01J20/06B01J20/103B01J20/28007B01D15/08C07F9/103
Inventor 沈清赵巧灵王萍亚
Owner ZHEJIANG GONGSHANG UNIVERSITY
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