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Method for synergistically and efficiently preparing protein-based nano-emulsion by taking polyhydroxy alcohol as molecular chaperone and prepared protein-based nano-emulsion

A polyhydric alcohol and nanoemulsion technology, which is applied in the field of protein-based nanoemulsions, can solve the problems of easy denaturation, aggregation, emulsion stability decline, small kinetic particle size, etc., and achieve excellent protection or controlled release effects and emulsification efficiency High effect with low equipment requirements

Active Publication Date: 2020-12-08
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aiming at the shortcomings of protein nanoemulsions that are difficult to prepare and have poor stability, some strategies and technologies have been tried at home and abroad to solve them, but overall, the results are not very good
Globulin with small molecular weight (such as whey protein) has certain advantages in forming nanoemulsions due to its good interface, emulsifying activity and small dynamic particle size, such as smaller dosage and lower homogeneous strength, but It is highly prone to denaturation and aggregation during the high-pressure process, which will lead to a decrease in the stability of the corresponding emulsion
Then, can some simple physical and chemical methods be used to promote the formation of protein nanoemulsion and improve its stability? This is an important scientific and technological issue that needs to be broken through in related fields.

Method used

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  • Method for synergistically and efficiently preparing protein-based nano-emulsion by taking polyhydroxy alcohol as molecular chaperone and prepared protein-based nano-emulsion
  • Method for synergistically and efficiently preparing protein-based nano-emulsion by taking polyhydroxy alcohol as molecular chaperone and prepared protein-based nano-emulsion
  • Method for synergistically and efficiently preparing protein-based nano-emulsion by taking polyhydroxy alcohol as molecular chaperone and prepared protein-based nano-emulsion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A method (experimental group) for synergistically and efficiently preparing protein-based nanoemulsions with polyhydric alcohols as molecular chaperones, comprising the steps of:

[0039] (1) Accurately weigh 1g of BSA powder (bovine serum albumin powder), disperse it in 99g of sucrose solution with a mass concentration of 40wt%, and stir continuously at room temperature for 2h to fully disperse the protein particles to obtain a protein dispersion, Adjust the pH to 7.0, add sodium azide to prevent microbial growth, the mass concentration of sodium azide in the protein dispersion is 0.02g / 100g, and place the obtained protein dispersion at 4°C for 12 hours to fully hydrate the protein , to obtain a BSA stock solution with a protein mass concentration of 1 g / 100 g.

[0040] (2) Before preparing the emulsion, stir the BSA stock solution (BSA dispersion) at room temperature for 0.5 h to return the temperature to room temperature and promote the uniform dispersion of the prot...

Embodiment 2

[0055] According to the method of the experimental group in Example 1, WPI-stabilized nanoemulsions were prepared, and the protein-removed samples were replaced with WPI powder (whey protein powder), and the homogenization times were replaced with 1 time and 6 times respectively, and other operations were the same as in Example 1. The control group in Example 2 is the protein-based nanoemulsion obtained by using the method of the experimental group in Example 2 only by using distilled water instead of 40 wt% sucrose.

[0056] According to the scheme of this example, a stable protein-based nanoemulsion can still be efficiently prepared (Table 3). It is worth noting that due to the excellent foaming properties of WPI, small bubbles are inevitably formed during the homogenization process, and the particle size of the obtained fresh nanoemulsion is slightly larger than that of the nanoemulsion placed at room temperature for 10 hours. The contrast operation in the embodiment thus c...

Embodiment 3

[0058] According to the experimental group method of embodiment 1, prepare the stable nanoemulsion of SPI, remove protein sample and change to SPI powder (soybean protein isolate powder), homogenization number of times is changed to 1 time and 6 times respectively, other operations are the same as embodiment 1. The control group in Example 3 is a protein-based nanoemulsion obtained by using the method of the experimental group in Example 3, only using distilled water instead of 40 wt% sucrose.

[0059] According to the scheme of this example, a stable protein-based nanoemulsion can still be efficiently produced (as shown in Table 3 below). Under the condition of high concentration of sucrose, the performance of the freshly prepared SPI-stabilized nanoemulsion is similar to that of the WPI-stabilized nanoemulsion (refer to Figure 2b shown), Figure 2b After being processed 1 time or 6 times by 80MPa microjet in embodiment 2, the particle size distribution figure of the emulsi...

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Abstract

The invention discloses a method for synergistically and efficiently preparing a protein-based nano-emulsion by taking polyhydroxy alcohol as a molecular chaperone and the prepared protein-based nano-emulsion. The method comprises the following steps of dissolving protein in a polyhydroxy alcohol solution, performing uniform mixing to obtain a protein dispersion solution, performing mixing and homogenizing with an oil phase to obtain a crude emulsion, and performing emulsifying to obtain the protein-based nano-emulsion. According to the method, the functional characteristic that a polyhydroxycompound can be used as an active protective agent of enzyme or other functional proteins is utilized, and the protein and polyhydroxy alcohol are premixed, so that the original conformation of the protein in the subsequent high-energy emulsification process is effectively protected; and mixing with an oil phase is performed, and high-pressure homogenization is performed to obtain nano-scale oil-in-water emulsion with uniform particle size and good stability. The protein-based nano-emulsion provided by the invention is simple in materials, is food-grade, can be directly obtained through a convenient homogenizing means, and has an excellent protection or controlled release effect on the oil phase, so that the protein-based nano-emulsion has a wide application prospect in the fields of dailychemical products, foods, medicines and the like.

Description

technical field [0001] The invention belongs to the technical field of emulsion preparation, and in particular relates to a method for synergistically and efficiently preparing protein-based nanoemulsions with polyhydric alcohols as molecular chaperones and the prepared protein-based nanoemulsions. Background technique [0002] Nanoemulsions usually refer to emulsions with a particle size of 20-200nm, because such systems not only have better stability and light transmittance than ordinary emulsions, but also can significantly improve the bioavailability or Potency, if the volatile essential oil is used as the oil phase for nano-embedding, it can not only improve its storage stability, but also significantly increase its solubility and antibacterial activity in the water phase, so it is used as an embedding delivery carrier for fat-soluble bioactive substances , nano-coating, food additives, transdermal drug delivery and other aspects have broad application prospects. Howev...

Claims

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Application Information

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IPC IPC(8): A23L29/10A23L33/19A23L33/17A23L33/185A23J3/10A23J3/12A23J3/04A23J3/08A23J3/14A23J3/16A61K38/17A61K38/47A61K38/01A61K38/38A61K47/10A61K47/26A61K47/44A61K9/107
CPCA23L29/10A23L33/19A23L33/17A23L33/185A23J3/10A23J3/12A23J3/04A23J3/08A23J3/14A23J3/16A61K38/1709A61K38/47A61K38/011A61K38/385A61K38/38A61K47/10A61K47/26A61K47/44A61K9/1075C12Y302/01017A23V2002/00A23V2200/222A23V2250/54246A23V2250/542A23V2250/5428A23V2250/5424A23V2250/548A23V2250/5488A23V2250/54252A61K2300/00
Inventor 唐传核韩雯许彦腾
Owner SOUTH CHINA UNIV OF TECH
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