Method for synergistically and efficiently preparing protein-based nano-emulsion by taking polyhydroxy alcohol as molecular chaperone and prepared protein-based nano-emulsion

Abstract

The invention discloses a method for synergistically and efficiently preparing a protein-based nano-emulsion by taking polyhydroxy alcohol as a molecular chaperone and the prepared protein-based nano-emulsion. The method comprises the following steps of dissolving protein in a polyhydroxy alcohol solution, performing uniform mixing to obtain a protein dispersion solution, performing mixing and homogenizing with an oil phase to obtain a crude emulsion, and performing emulsifying to obtain the protein-based nano-emulsion. According to the method, the functional characteristic that a polyhydroxycompound can be used as an active protective agent of enzyme or other functional proteins is utilized, and the protein and polyhydroxy alcohol are premixed, so that the original conformation of the protein in the subsequent high-energy emulsification process is effectively protected; and mixing with an oil phase is performed, and high-pressure homogenization is performed to obtain nano-scale oil-in-water emulsion with uniform particle size and good stability. The protein-based nano-emulsion provided by the invention is simple in materials, is food-grade, can be directly obtained through a convenient homogenizing means, and has an excellent protection or controlled release effect on the oil phase, so that the protein-based nano-emulsion has a wide application prospect in the fields of dailychemical products, foods, medicines and the like.

Description

technical field

[0001] The invention belongs to the technical field of emulsion preparation, and in particular relates to a method for synergistically and efficiently preparing protein-based nanoemulsions with polyhydric alcohols as molecular chaperones and the prepared protein-based nanoemulsions. Background technique

[0002] Nanoemulsions usually refer to emulsions with a particle size of 20-200nm, because such systems not only have better stability and light transmittance than ordinary emulsions, but also can significantly improve the bioavailability or Potency, if the volatile essential oil is used as the oil phase for nano-embedding, it can not only improve its storage stability, but also significantly increase its solubility and antibacterial activity in the water phase, so it is used as an embedding delivery carrier for fat-soluble bioactive substances , nano-coating, food additives, transdermal drug delivery and other aspects have broad application prospects. Howev...

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