Alkaline phosphatase-catalyzed chemiluminescent substrate enhancer and application thereof

A phosphatase-catalyzed chemical and luminescent substrate technology, applied in the field of immunodetection, can solve the problems affecting the detection accuracy of luminescence value, low enhancement multiple of enhancer, uneven luminescence rate, etc. Enhanced, low critical micelle concentration effect

Active Publication Date: 2020-12-11
WUHAN LIFE ORIGIN BIOTECH LTD
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] 1,2-dioxetane compound (AMPPD) is an ultrasensitive alkaline phosphatase substrate, under the catalysis of alkaline phosphatase (AP), the phosphate group is hydrolyzed to form an unstable Intermediate, self-decomposing, -O-O- four-membered ring breaks and releases a large amount of energy, thereby stimulating chemiluminescence reaction and emitting photons. Although its signal can last for more than 20 hours, its luminescence rate is low. Commercially available chemiluminescence substrates The solution is a substrate solution compounded with an enhancer and AMPPD to increase the luminous intensity
It is

Method used

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  • Alkaline phosphatase-catalyzed chemiluminescent substrate enhancer and application thereof
  • Alkaline phosphatase-catalyzed chemiluminescent substrate enhancer and application thereof
  • Alkaline phosphatase-catalyzed chemiluminescent substrate enhancer and application thereof

Examples

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Example Embodiment

[0027] Example 1

[0028] This embodiment provides an alkaline phosphatase-catalyzed chemiluminescence substrate enhancer, specifically: water-soluble quantum dots with a mass ratio of 1:20 and Gemini surfactant, wherein the Gemini surfactant is Gemini C12, and its structural formula for: where R group is C 12 H 25 Alkyl; water-soluble quantum dots are water-soluble quantum dots CdTe.

[0029] Gemini surfactant, that is, gemini surfactant, is a surfactant formed by linking the ionic head groups of two traditional surfactants through chemical bonds, and the two ionic heads are closely linked; this strong interaction effectively The repulsive effect of the charge of the ionic head group is prevented, and the polar groups are arranged more closely. This structure strengthens the hydrophobic binding force between the hydrocarbon chains, and the repulsive force between the ionic head groups is affected by the chemical bond force. suppressed and greatly weakened. Therefore, co...

Example Embodiment

[0034] Example 2

[0035] The difference between this example and Example 1 is that the mass ratio of water-soluble quantum dots and Gemini surfactant in the enhancer is 1:15.

Example Embodiment

[0036] Example 3

[0037] The difference between this example and Example 1 is that the mass ratio of water-soluble quantum dots and Gemini surfactant in the enhancer is 1:30.

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Abstract

The invention discloses an alkaline phosphatase-catalyzed chemiluminiscence substrate enhancer and an application thereof. The alkaline phosphatase-catalyzed chemiluminiscence substrate enhancer comprises a fluorescent compound and a surfactant, the fluorescent compound is water-soluble quantum dots, the surfactant is a Gemini surfactant, and a chemiluminiscence substrate solution containing the enhancer comprises 50-500 mM of 2-amino- 2-methyl-1-propanol, 0.1-1 mM of AMPPD, 1-10 mM of MgCl2, 10-30 mg/L of the enhancer and 100-1000 mg/L of preservative. The Gemini surfactant is creatively selected to be matched with the water-soluble quantum dots to obtain the alkaline phosphatase-catalyzed chemiluminescence substrate enhancer, a distance between the water-soluble quantum dots and the AMPPD substrate is shortened by utilizing an internal hydrophobic microenvironment of micelles formed by the Gemini surfactant, and then light energy transfer is generated; and a quantum yield of the water-soluble quantum dots is far higher than that of chemiluminescence molecules so that a luminescence value of the chemiluminescence substrate liquid containing the enhancer is remarkably enhanced, andperformances of luminescence value measurement, such as precision, sensitivity and luminescence rate stability, are further increased.

Description

technical field [0001] The invention belongs to the technical field of immunoassay, and in particular relates to an alkaline phosphatase-catalyzed chemiluminescence substrate enhancer and application thereof. Background technique [0002] Chemiluminescence is a kind of light radiation phenomenon accompanied by the chemical reaction of substances, which can be divided into direct luminescence and indirect luminescence. Direct luminescence is the simplest chemiluminescence reaction, which consists of two key steps: excitation and radiation. For example, two substances A and B undergo a chemical reaction to form C substance, the energy released by the reaction is absorbed by the molecules of C substance and transitions to the excited state C*, and the excited C* generates light radiation in the process of returning to the ground state. Here C* is a luminescent body, and because C directly participates in the reaction in this process, it is called direct chemiluminescence. Ind...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N33/58G01N33/574G01N33/543
CPCG01N21/76G01N33/581G01N33/57476G01N33/54326
Inventor 李重阳来祥兵舒芹张雪娇
Owner WUHAN LIFE ORIGIN BIOTECH LTD
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