Plant growth-promoting strain XN-K13 and application thereof

An XN-K13, a technology for promoting plant growth, applied in the directions of plant growth regulators, plant growth regulators, applications, etc., can solve problems such as reduced utilization rate, and achieve the goal of improving nutritional balance, increasing the content of available potassium, and promoting plant growth. Effect

Active Publication Date: 2020-12-22
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Chemical fertilizers play a huge role in modern agricultural production, but with th

Method used

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  • Plant growth-promoting strain XN-K13 and application thereof
  • Plant growth-promoting strain XN-K13 and application thereof
  • Plant growth-promoting strain XN-K13 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Isolation and identification of plant growth promoting strain XN-K13

[0038] 1.1 Isolation of plant growth-promoting strain XN-K13

[0039] The isolation of plant growth-promoting strain XN-K13 includes three steps: sampling, enrichment, primary screening, and secondary screening, as follows:

[0040] 1.1.1 Sampling

[0041]Samples were taken from the soil of Guangxi Medicinal Botanical Garden (E108°19', N22°51'), put into clean sampling bags, marked, and stored in a refrigerator at 4°C as rhizosphere soil samples.

[0042] 1.1.2 Enrichment and primary screening

[0043] Weigh 1.0 g of rhizosphere soil sample, put it into a test tube filled with 9 mL of sterile deionized water, shake and mix thoroughly, 37 ° C, 200 r / min, shake and cultivate for 30 min, and dilute it in a ten-step gradient to obtain a dilution; To isolate salt-tolerant bacteria, add NaCl with a mass concentration of 10g / L as salt stress, draw 100uL of the diluted solution and spread it on beef extra...

Embodiment 2

[0062] Accurate characterization of potassium-solubilizing plant growth-promoting strain XN-K13:

[0063] The dissolving ability of Enterobacterludwigii XN-K13 to poorly soluble potassium was preliminarily evaluated by the improved selection medium for potassium-solubilizing bacteria: use a sterile toothpick to puncture the isolated and purified strain obtained in 1.1. Potassium feldspar was used as the insoluble potassium source in the center of the improved selective medium for potassium-dissolving bacteria, placed in a 37°C incubator and cultured for 5 days, each group was treated three times, and the potassium-dissolving circle was observed to measure the colony diameter (d) and the potassium-dissolving circle diameter ( D), the results are as shown in table 2, calculate the ratio of solution potassium circle and bacterium colony diameter ( Figure 4 ); from Table 2 and Figure 4 It can be concluded that the ratio of the potassium-dissolving circle to the colony diameter ...

Embodiment 3

[0067] Quantitative Analysis of Potassium Solution by Plant Growth-promoting Strain XN-K13:

[0068] Potassium standard solution: Accurately weigh 1.9068g of analytically pure KCl that has been dried (dried at 105°C for 4-6 hours), dissolve it in 200mL of deionized water, and set the volume to 1L. Shake well to obtain 1000mg / L of potassium. Put 25mL of the solution into a 250mL volumetric flask to constant volume and shake well, then it will be a potassium standard solution containing 100mg / L of potassium.

[0069] 3% sodium tetraphenylborate solution: Weigh 3g sodium tetraphenylborate and dissolve it in 100mL deionized water, add 10 drops of 0.2mol / L sodium hydroxide solution to adjust the pH to 8-9, let it stand overnight, and filter it with dense filter paper Filter and store in a brown bottle.

[0070] 0.05mol / L borax solution: Weigh 19.07g borax and dissolve it in 1000mL deionized water.

[0071] Formaldehyde-EDTA solution: Weigh 2.5g EDTA disodium salt and dissolve it ...

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Abstract

The invention discloses a plant growth-promoting strain XN-K13, which is classified and named as Enterobacter ludwigii XN-K13, is preserved in China Center for Type Culture Collection on May 25th, 2020, has an address of Luojia Mountain in Wuhan City, Hubei Province, China, and has a preservation number of CCTCC NO: M 2020145. The plant growth-promoting strain XN-K13 provided by the invention canbe used for synthesizing plant growth hormone indoleacetic acid (IAA) by taking tryptophan as a precursor, can be directly utilized by plants because of being adsorbed on the surfaces of seeds and root systems, and can also be used for stimulating the growth and proliferation of plant cells under the combined action of the plant growth-promoting strain XN-K13 and the IAA endophytic in the plants;therefore, the growth and development of plant roots are promoted, moisture and nutrients in soil are effectively absorbed, and other life activities in plants are regulated.

Description

technical field [0001] The invention belongs to the technical field of plant growth-promoting bacteria, and specifically relates to a plant growth-promoting bacteria strain XN-K13 that can decompose insoluble potassium and its application. Background technique [0002] Chemical fertilizers play a huge role in modern agricultural production, but with the increase in the use of chemical fertilizers, their utilization rate decreases year by year. Residues of pesticides and chemical fertilizers release a large amount of harmful substances to the environment, pollute soil, water sources and food, and pose a great threat to human health and living environment. The call for protecting the ecological environment and producing safe food is becoming stronger and stronger. Therefore, the development and utilization of new fertilizer sources that replace chemical fertilizers to meet the needs of the development of green agriculture and green food has become a top priority. Microbial fe...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/20A01P21/00C05G3/00C05G3/40C05G3/80C12R1/01
CPCA01N63/20C05G3/00C05G3/40C05G3/80C12R2001/01C12N1/205
Inventor 李有志樊宪伟黄巧
Owner GUANGXI UNIV
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