Method for preparing crude heparin sodium from porcine small intestine mucous membrane with low salt

A crude heparin, pig small intestine technology, applied in the field of crude heparin sodium extraction technology, can solve problems such as high-salt wastewater pollution, and achieve the effects of strong operability, reduced salt concentration, and complete separation

Active Publication Date: 2021-01-01
河北常山凯库得生物技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] In view of this, the present invention aims to propose a method for preparing crude heparin sodium using low-salt pig small intestinal mucosa, so as to solve the problem of high-salt wastewater pollution under the premise of ensuring the quality of crude heparin sodium

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  • Method for preparing crude heparin sodium from porcine small intestine mucous membrane with low salt
  • Method for preparing crude heparin sodium from porcine small intestine mucous membrane with low salt

Examples

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Embodiment 1

[0042] This example relates to a method for preparing crude heparin sodium using low-salt porcine small intestinal mucosa, including low-salt compound enzymolysis to obtain enzymolysis solution, enzymolysis solution concentration to obtain concentrated solution, ion exchange to obtain crude heparin sodium eluate and alcohol lake There are four steps in drying to obtain heparin sodium.

[0043] Step 1, low-salt compound enzymatic hydrolysis: prepare the mucous membrane solution of the pig small intestine, add saturated saline solution, adjust the salt concentration of the mucous membrane solution to 0.8-1.0% by mass, adjust the pH of the mucous membrane solution to 7.0-8.0; raise the temperature to 50-55°C, add complex enzymes for enzymolysis to obtain an enzymolysis solution. Specifically, first put the fresh porcine small intestine mucosal solution into the enzymatic hydrolysis reaction tank, preferably, the mass ratio of mucous membrane to water in the porcine small intestin...

Embodiment 2

[0062] This example relates to a method for preparing crude heparin sodium using low-salt porcine small intestinal mucosa, which is a specific application under Example 1.

Embodiment 21

[0064] 1.1 Put the mucous fluid scraped from pig small intestines into the enzymatic reaction tank, the number of small intestines is 1450, and the volume is 10700L.

[0065] 1.2 Turn on the stirring, add 400L of saturated casing saline to the enzymolysis reaction tank, set the volume to 12050L, and adjust the salt concentration of the mucous membrane to 0.91%.

[0066] 1.3 Add 37L of 25% NaOH solution to the enzymolysis reaction tank, and use PH5.5-9.0 precision test paper to measure the pH to 7.5.

[0067] 1.4 Turn on the steam and raise the temperature to 52°C. Add 2.7kg of complex protease and keep warm for 2h. Raise the temperature to 83°C and keep it warm for 25 minutes. Cool down to 55°C with cooling water.

[0068] 1.5 Add 40kg of anion resin to the enzymolysis reaction tank, stir to absorb heparin for 8 hours, and collect the resin with an 80-mesh filter bag.

[0069] 1.6 Wash the resin with 72L of 2% NaCl solution by mass fraction to remove impurities such as pro...

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Abstract

The invention provides a method for preparing crude heparin sodium from porcine small intestine mucosa with low salt. The method comprises the following steps: carrying out low-salt composite enzymolysis to obtain an enzymatic hydrolysate, concentrating the enzymatic hydrolysate to obtain a concentrated solution, carrying out ion exchange to obtain a crude heparin sodium eluent, carrying out alcohol precipitation, and performing drying to obtain the crude heparin sodium, to be specific, adding saturated casingsaline water in thelow-salt composite enzymolysis; and adjusting the salt concentration of the mucous membrane solution to 0.8-1.0% by mass. According to the method for preparing the crude heparin sodium from the porcine small intestine mucous membrane in the low-salt mode, enzymolysis and adsorption processes of the crude heparin sodium can be achieved under the low-salt condition, the salt concentration in discharged wastewater can be reduced while the yield of the crude heparinsodium is guaranteed, the wastewater treatment cost is reduced, environmental pollution is reduced, and comprehensive economic benefits are increased.

Description

technical field [0001] The invention relates to the technical field of extracting crude heparin sodium from animal casings, in particular to a method for preparing crude heparin sodium using low-salt pig small intestine mucosa. Background technique [0002] Heparin is a mucopolysaccharide that widely exists in mammalian tissues, mainly exists in mast cells, has anticoagulant effect, and is widely used in the treatment of surgery, cerebral thrombosis, myocardial infarction, etc. The molecular weight of heparin is about 3000-37500, and research on commercial heparin suggests that there are at least 21 molecular entities. Heparin sodium is the sodium salt of heparin. It is the most widely used anticoagulant drug in the field of clinical medicine. It is composed of D-glucosamine, N-acetylglucosamine, D-glucuronic acid and L-iduronic acid alternately. Composed of mucopolysaccharide sulfate, is widely used in the prevention and treatment of cerebral thrombosis. Heparin sodium is...

Claims

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Application Information

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IPC IPC(8): C08B37/10
CPCC08B37/0075C08B37/0003Y02P20/10
Inventor 张朝阳乔康康崔洁高秀强冯朝
Owner 河北常山凯库得生物技术有限公司
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