Method for preparing biocontrol bacterial fertilizer by fermenting perishable waste with bacillus velezensis

A Bacillus and waste technology, applied in the field of bio-control bacterial fertilizer, can solve the problem of no anti-bacterial fertilizer and other problems, achieve good biological control effect, reduce production cost, solve the effect of harmless treatment and resource utilization

Active Publication Date: 2021-01-01
CHANGSHU INSTITUTE OF TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] After searching, there is currently no report on the use of B. velezensis to ferment organic wastes to prepare biocontrol bacterial fertilizers.

Method used

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  • Method for preparing biocontrol bacterial fertilizer by fermenting perishable waste with bacillus velezensis
  • Method for preparing biocontrol bacterial fertilizer by fermenting perishable waste with bacillus velezensis
  • Method for preparing biocontrol bacterial fertilizer by fermenting perishable waste with bacillus velezensis

Examples

Experimental program
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Effect test

Embodiment 1

[0059] Example 1: Isolation and identification of B. velezensis LT-2.

[0060] In the early stage, a strain with fast growth and high cellulase activity was found in distiller's yeast. In order to identify the strain, the genomic DNA of B. Primer 27F and downstream primer 1492R PCR amplified 16S rDNA sequence as figure 1 As shown in A, the PCR-amplified product was gel-recovered and purified, and the gel-recovered and purified product was sent to Suzhou Jinweizhi Biotechnology Co., Ltd. for sequencing. The nucleotide sequence length of the 16S rDNA gene of the strain obtained by sequencing is 1396bp, and its gene sequence is shown in SEQID No.1. The sequencing results were compared with the known 16S rDNA sequences in the GeneBank database, and the BLAST program was used for homology comparison to construct a phylogenetic tree based on the complete 16S rDNA sequences. The results showed that the strain reached 100% homology with B. velezensis zjt9 ( figure 1 B). According ...

Embodiment 2

[0061] Embodiment 2: the optimization of manganese ion concentration in solid-state song seed culture medium and seed liquid culture medium

[0062] According to reports Mn 2+ It is beneficial to the generation of Bacillus velei spores, so the concentration of manganese ions in the solid koji culture medium and the seed liquid medium is optimized. B. velezensis LT-2 were inoculated in different amounts of Mn 2+ (0.25, 0.50, 0.75, 1.00, 1.25, 1.50g / kg) solid-state koji culture medium and seed liquid medium, the initial pH value was 7.5, the culture temperature was 32°C, and the culture time was 48h, and then the number of spores was determined. number. It was found that adding 1.00g / kg of Mn to the solid koji culture medium 2+ When the number of spores reaches the maximum, it is 8.2×10 9 individual / mL; 0.75g / kg of Mn was added to the seed liquid medium 2+ When the number of spores reaches the maximum, it is 8.5×10 9 individual / mL. Therefore, the best Mn in solid koji and...

Embodiment 3

[0063] Example 3: Optimization of rice husk content prepared by fermentation of Bacillus veleisi LT-2

[0064] Bacillus fermentation belongs to aerobic fermentation, and a large amount of oxygen is needed in the fermentation process. According to literature reports, adding rice husk can increase the gap between solid substrates, allowing air to enter to ensure the progress of fermentation. This example illustrates the influence of different rice husk additions on bacterial strain fermentation to prepare biocontrol bacterial fertilizers. The seed culture solution is inoculated on rice husks with 25% (v / w) inoculation amount respectively. %, 8%, 10%, 12% (w / w) biocontrol bacterial fertilizer preparation fermentation system for solid-state fermentation, found that when the addition of rice husk was 6%, the solid-state fermentation temperature rise rate was the fastest, although the required fermentation time short. Therefore, 6% of rice husk was selected as the optimal addition ...

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Abstract

The invention discloses application of bacillus velezensis to preparation of a biocontrol bacterial fertilizer by fermenting perishable waste and a preparation method, and belongs to the technical field of biocontrol bacterial fertilizer preparation. The classification name of the bacillus velezensis is bacillus velezensis LT-2, the bacillus velezensis LT-2 has been collected in China Center for Type Culture Collection, the collection number of the bacillus velezensis registered in a library is CCTCC No:M2019904, and the collection date is November 7, 2019. The method mainly comprises the following technical processes: preparing solid yeast seeds, preparing a compost fermentation seed solution and preparing the biocontrol bacterial fertilizer through solid-state fermentation. According tothe method, the efficient agricultural biocontrol bacterial fertilizer is prepared by taking the organic perishable waste as a raw material, so that the progress of replacing chemical pesticides witha biological product is accelerated, and the problem of crop "food safety" brought by long-term dependence on the chemical pesticides is solved, and the bacillus velezensis has important significancefor promoting sustainable development of modern agriculture in China.

Description

technical field [0001] The invention specifically relates to the application of Bacillus velaisi in the preparation of bio-control bacterial fertilizer by fermenting decomposing waste, and belongs to the technical field of bio-control bacterial fertilizer. Background technique [0002] Due to the long-term excessive use of chemical fertilizers and pesticides, the cultivated land has been seriously polluted, such as thinning of the cultivated layer, soil compaction and acidification, insufficient organic matter content, nutrient imbalance, pesticide pollution, soil ecological function deterioration, etc., which seriously damages the soil ecosystem and directly threatens the human body. healthy. According to reports, the application of organic fertilizers can provide crops with a stable, sufficient and continuous nutrient supply, thereby promoting crop growth and increasing production, and the market prospect is good. Therefore, the selection of natural, harmless and environm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/00C05G3/60C12R1/07
CPCC12N1/20C05G3/00C05G3/60C05D9/00C12R2001/07C12N1/205C05F1/002C05F1/005C05F3/00C05F5/002C05F11/00C05F17/20
Inventor 吴凌天李双红李好樱子马醒佳丁舒稳董柳胡晨洋吴金男
Owner CHANGSHU INSTITUTE OF TECHNOLOGY
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