Lactobacillus plantarum strain LDVS007 and application thereof

A technology of Lactobacillus plantarum and strains, applied in the field of microorganisms, can solve problems such as the influence of lactic acid bacteria activity, influence on lactic acid bacteria colonization, and regulation effect

Active Publication Date: 2021-01-08
田林县百花八渡笋加工厂
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Lactobacillus plantarum is a probiotic commonly used in humans or animals, and has a two-way regulating effect on the intestinal tract. At present, there are many processed products of lactic acid bacteria on the market, such as yogurt, bacteria powder and other finished products, but Lactobacillus plantarum is passing through In the process of the human intestinal tract, the bile salts, acids and other substances in the human body will have a certain impact on the activity of lactic acid bacteria, thus affecting the colonization and regulation of lactic acid bacteria. Therefore, it is necessary to further screen bile salt-resistant and acid-resistant lactic acid bacteria to obtain Higher value lactic acid bacteria germplasm resources; in order to improve the preservation and transportation capacity of the strains, the prior art usually adopts the method of preparing the strains into freeze-dried powder to apply the strains, but it is found in the prior art that although the strains are the same strains, but not all protective agents are fully applicable, the choice of protective agent in lyophilized powder is related to the strain type of the strain itself

Method used

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  • Lactobacillus plantarum strain LDVS007 and application thereof
  • Lactobacillus plantarum strain LDVS007 and application thereof
  • Lactobacillus plantarum strain LDVS007 and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] This embodiment is the screening method of Lactobacillus plantarum (Lactobacillus plantarum) strain LDVS007:

[0034] The bacterial strain of this embodiment is isolated from sour cowpea, and sour cowpea is taken from the market in Nanning, Guangxi, and is obtained by using the MRS medium plate coating method and the method of slanting lines to separate it, and finally screened out bacterial strains LDVS012, LDVS007, LDVS008, and LDVS005 . After testing, the applicant found that the strain LDVS007 has the highest tolerance to bile salts and has the highest antioxidant capacity among the four strains.

[0035] For the strains in this example, use the NCBI Blast program to compare the spliced ​​sequence files with the data in the NCBI 16S database, and construct a phylogenetic tree through MEGA7.0 for the comparison results. The results showed that the homology between strain LDVS007 and Lactobacillus plantarum was 98.67%. According to the physiological and biochemical ...

Embodiment 2

[0038] Identification of growth and acid production capacity of strains LDVS005, LDVS007, LDVS008, LDVS012:

[0039] In the Erlenmeyer flask equipped with 100mL liquid MRS, insert the seed fermentation liquid according to the inoculum size of 2% (v / v), and cultivate it under the condition of 30 ℃ for 36h, respectively at 0, 1, 3, 6, 9, 12, Measure the OD value at a wavelength of 600nm at 24 and 36h, and draw a growth curve; the results are as follows image 3 Shown: 4 strains of lactic acid bacteria are in the lag phase in the growth phase at 0-3h, enter the logarithmic growth phase after 3h, and enter the stable phase after 12h. The results showed that the growth and reproduction of lactic acid bacteria were more vigorous before 12 hours, and compared with Figure 4The characteristics of the pH value change curve of lactic acid bacteria just coincided. At 12h, the absorbance value of LDVS012 at 600nm was significantly higher than that of the other three strains (P0.05), and...

Embodiment 3

[0046] Tests for tolerance of strains to bile salts:

[0047] After 24 hours of activation, the lactic acid bacteria were inoculated in MRS medium with bile salt content of 3 g / L, 3.5 g / L and 4.0 g / L according to the inoculum size of 2%, and incubated at 30° C. for 24 hours at a constant temperature. Determine the survival rate of 4 strains: LDVS005, LDVS007, LDVS008, LDVS012 and a commercial bacterial agent (purchased from Taobao) on the market, as shown in Table 2:

[0048] Table 2 Bile salt tolerance of lactic acid bacteria

[0049] strain 3g / L bile salt 3.5g / L bile salt 4.0g / L bile salt LDVS005 78.3％ 53.5％ 35.2％ LDVS007 88.6％ 75.3％ 52.6％ LDVS008 61.2％ 42.1％ 18.7％ LDVS012 46.3％ 25.3％ 10.2％ commercial bacteria 48.4％ 24.6％ 11.6％

[0050] It can be seen from Table 2 that at a concentration of 3 g / L bile salts, the bacterial strains LDVS005, LDVS007 and LDVS008 can all achieve a survival rate of more than 50%, and the...

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Abstract

The invention relates to the technical field of microorganisms, in particular to a lactobacillus plantarum strain LDVS007 and application thereof. The LDVS007 strain is separated from preserved cowpeas with chili in the markets of Nanning, and has high cholate resistance and oxidation resistance. In order to improve the oxidation resistance of the strain, the inventor conducts single-factor and orthogonal experiments, and finally finds out traditional Chinese medicine preparations including a kadsura coccinea peel extract, a grape peel extract and an aloe extract which can all promote the oxidation resistance of the LDVS007 strain. The extracts are matched with a freeze-drying protective agent to process the strain LDVS007 into freeze-drying powder, and the effects of facilitating transportation and storage and improving the survival rate and oxidation resistance of the strain can be achieved. If the strain is applied to animal bodies, the colonization capacity of lactic acid bacteriain the animal bodies can be greatly improved, and good intestinal tract adjusting and health care effects are achieved.

Description

【Technical field】 [0001] The invention relates to the technical field of microorganisms, in particular to Lactobacillus plantarum strain LDVS007 and applications thereof. 【Background technique】 [0002] Lactobacillus plantarum (Lactobacillus plantarum), belonging to Gram-positive strains, is a kind of bacterium with curved rod shape, round-ended short rod shape and chain shape, and does not produce spores. Facultative lactic acid bacteria, which produce fructose, Glucose, lactose, and xylose are used as raw materials for metabolic growth to produce acid. They are one of the indispensable strains in the production of fermented products. They are commonly used in the production of kimchi such as cowpea, cabbage, and onion. [0003] Lactobacillus plantarum is a probiotic commonly used in humans or animals, and has a two-way regulating effect on the intestinal tract. At present, there are many processed products of lactic acid bacteria on the market, such as yogurt, bacteria pow...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04A23L33/135A61K36/896A61K36/886A61K35/747A61P1/00A61P39/06A61P37/04C12R1/25
CPCC12N1/20C12N1/04A23L33/135A61K35/747A61K36/79A61K36/57A61K36/87A61K36/896A61K36/886A61P1/00A61P39/06A61P37/04A23V2002/00C12R2001/25C12N1/205A23V2400/169A61K2300/00A23V2200/3204A23V2200/324
Inventor 辛明李昌宝李丽何雪梅孙健李杰民黄洁琳孙宇刘国明唐杰盛金凤
Owner 田林县百花八渡笋加工厂
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