A kind of magnetic nano-microsphere of glucose detection reagent and preparation method thereof

A magnetic nanometer and detection reagent technology, which is applied in biochemical equipment and methods, measuring devices, microbial determination/inspection, etc. Accuracy, improve detection speed, and prolong the effect of storage time

Active Publication Date: 2022-06-17
南京迪安医学检验所有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Among them, the ninth method is urine albumin excretion. The urine albumin excretion can be detected sensitively by the enzyme-linked method. The urine sugar detection method is the most convenient and the cheapest; but the accuracy of the urine test is not high, because the reagent is in the The stability is low during the transmission process. The longer the storage time of the detection reagents on the market, the less obvious the color change and the lower the accuracy. This requires the detection agency to continuously purchase new detection reagents for detection, which increases the cost and reduces the detection. Quality; the market needs a method that can enhance the stability of detection reagents, increase the storage time of detection reagents, and reduce storage requirements

Method used

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  • A kind of magnetic nano-microsphere of glucose detection reagent and preparation method thereof
  • A kind of magnetic nano-microsphere of glucose detection reagent and preparation method thereof

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preparation example Construction

[0043] A preparation method of magnetic nano-microspheres of glucose detection reagent, comprising the following steps:

[0044] Prepare materials according to the recipe;

[0045] The formula includes: 26-42 parts of gelatin, 18-34 parts of quaternized chitosan, 2-6 parts of lecithin, 16-28 parts of sodium alginate, 80-160 parts of anhydrous ethanol, the concentration is 7000-8000U / L 20-30 parts of glucose oxidase, 10-20 parts of dehydrogenase with a concentration of 300-400U / L, 10-20 parts of o-benzidine with a concentration of 6-8.0mmol / L, 9-15 parts of surfactant parts, the concentration is 100-140mmol / L Tris buffer 60-120 parts;

[0046] Making Glucose Oxidase Magnetic Nanospheres:

[0047] Step 1, Microsphere polymer shell substrate:

[0048] Dissolve the gelatin in water at 80-90 degrees Celsius and stir, add the quaternized chitosan to the gelatin solution, stir, add lecithin, add sodium alginate, add magnetic nanoparticles, stir evenly, and finally add anhydrous E...

Embodiment 1

[0066] Example 1: 26g of gelatin, 22g of quaternized chitosan, 2g of lecithin, 16g of sodium alginate, 0.6g of iron oxide nanoparticles, 90g of absolute ethanol, 24g of glucose oxidase with a concentration of 7000-8000U / L, concentration 14g of dehydrogenase with a concentration of 300-400U / L, 16g of o-benzidine with a concentration of 6-8.0mmol / L, 11g of Tween 80, and 60g of 100-140mmol / L Tris buffer.

Embodiment 2

[0067] Example 2: 34g of gelatin, 34g of quaternized chitosan, 6g of lecithin, 28g of sodium alginate, 0.9g of iron oxide nanoparticles, 120g of absolute ethanol, 20g of glucose oxidase with a concentration of 7000-8000U / L, concentration 300-400U / L dehydrogenase 15g, 6-8.0mmol / L o-benzidine 15g, Tween 80 12g, 100-140mmol / L Tris buffer 80g.

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Abstract

The invention discloses a glucose detection reagent magnetic nano-microsphere and a preparation method thereof. The formula includes: 26-42 parts of gelatin, 18-34 parts of quaternized chitosan, 2-6 parts of lecithin, seaweed 16-28 parts of sodium bicarbonate, 0.6-1.2 parts of magnetic nanoparticles, 80-160 parts of absolute ethanol, 20-30 parts of glucose oxidase, 10-20 parts of dehydrogenase, 10-20 parts of o-benzidine, surface 9-15 parts of active agent, 60-120 parts of Tris buffer; the preparation method wraps each reagent individually with a microsphere polymer shell substrate, so that the reagent can maintain the stability of the reagent even under harsh transportation conditions , and enzyme activity, prolong the storage time of detection reagents, and improve detection accuracy.

Description

technical field [0001] The invention relates to the field of detection reagents, in particular to a magnetic nanometer microsphere of a glucose detection reagent and a preparation method thereof. Background technique [0002] Diabetes is a group of metabolic diseases characterized by hyperglycemia. Hyperglycemia is caused by defective insulin secretion, impaired biological action, or both. Long-term hyperglycemia leads to chronic damage and dysfunction of various tissues, especially the eyes, kidneys, heart, blood vessels, and nerves. [0003] There are generally nine ways to detect diabetes on the market, 1. blood sugar; 2. urine sugar; 3. urinary ketone bodies; 4. Glycated hemoglobin (HbA1c); 5. Glycated serum protein; 6. Serum insulin and C-peptide levels; Indicators; 9 urinary albumin excretion, radioimmunoassay or enzyme-linked method. [0004] The ninth method is urinary albumin excretion. The enzyme-linked method can sensitively detect the urinary albumin excretion...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/18C12N11/14C12N11/10C12N11/04C12Q1/54C12Q1/32C12Q1/26
CPCC12N11/18C12N11/14C12N11/10C12N11/04C12N9/0006C12Q1/54C12Q1/32C12Q1/26C12Y101/03004G01N2333/904
Inventor 朱思危
Owner 南京迪安医学检验所有限公司
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