Multi-color fluorescent carbon quantum dot as well as preparation method and application thereof
A carbon quantum dot and fluorescence technology, which is applied in the field of multicolor fluorescent carbon quantum dots and their preparation, can solve the problems of unstable bioimaging, toxic luminescence, and light damage in the biological field, and achieves that the purification method is simple, easy to operate, and easy to prepare. Low conditions and environmental requirements, less effect of acid and alkali environment
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Embodiment 1
[0028]Step 1, weigh 0.2g of ammonium citrate and 0.5g of urea in a reaction vessel, add 12mL of dimethylformamide, stir well to obtain a transparent clear solution, then transfer to a 30mL autoclave and put in an oven , the temperature was set to 150 degrees Celsius, kept for 14 hours, and after cooling to room temperature, a blue carbon quantum dot solution was obtained.
[0029] Step 2, the above-mentioned blue carbon quantum dot solution is transferred to a centrifuge tube, and after centrifugation at a speed of 10000 rpm for 10 minutes, the centrifuged liquid is filtered to obtain a clear liquid; a mixed solution of petroleum ether and ethyl acetate ( v:v=3:1) wash the supernatant, then shake the solution evenly, and sonicate. This process was repeated 2-3 times to obtain the precipitation of blue carbon quantum dots. Part of the precipitate was dispersed in ethanol to form a quantum dot colloidal solution. The remaining precipitates were dissolved in water and freeze-dr...
Embodiment 2
[0036] Step 1, weigh 0.4g of ammonium citrate and 0.4g of urea in a reaction vessel, add 12mL of dimethylformamide, stir well to obtain a transparent clear solution, then transfer to a 30mL autoclave, and place in an oven , the temperature was set to 170 degrees Celsius, kept for 14 hours, and after cooling to room temperature, a green carbon quantum dot solution was obtained.
[0037] Step 2, the above-mentioned green carbon quantum dot solution is transferred to a centrifuge tube, and after centrifugation at a speed of 10000 rpm for 10 minutes, the centrifuged liquid is filtered to obtain a clear liquid; a mixed solution of petroleum ether and ethyl acetate (v :v=3:1) Wash the supernatant, then shake the solution well, and sonicate. This process was repeated 2-3 times to obtain the precipitation of green carbon quantum dots. Part of the precipitate was dispersed in ethanol to form a quantum dot colloidal solution. The remaining precipitates were dissolved in water and free...
Embodiment 3
[0044] Step 1, weigh 1.4g of ammonium citrate and 0.8g of urea in a reaction vessel, add 12mL of dimethylformamide, stir well to obtain a transparent clear solution, then transfer to a 30mL autoclave and put in an oven , the temperature was set to 200 degrees Celsius, kept for 14 hours, and after cooling to room temperature, a red carbon quantum dot solution was obtained.
[0045] Step 2, transfer the above-mentioned red carbon quantum dot solution to a centrifuge tube, centrifuge at a speed of 10,000 rpm for 10 minutes, then filter the centrifuged liquid to obtain a clear liquid; use a mixed solution of petroleum ether and ethyl acetate (v :v=3:1) Wash the supernatant, then shake the solution well, and sonicate. This process was repeated 2-3 times to obtain the precipitation of red carbon quantum dots. Part of the precipitate was dispersed in ethanol to form a quantum dot colloidal solution. The remaining precipitates were dissolved in water and freeze-dried to obtain red c...
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