A Strain of Haemobacter and Its Application in Controlling Cucumber Fusarium Wilt
A cucumber fusarium wilt and bacillus technology, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of undisclosed technology for the prevention and treatment of Haemobacterium cucumber wilt, difficult colonization of bacteria, short survival time, etc., and achieve great application value, Easy-to-operate, simple-to-use effects
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Embodiment 1
[0032] Isolation and identification
[0033] The strain isolation samples were collected from the garlic field of Fanzhen, Tai'an City, Shandong Province, and the collected larvae were placed in the seal pocket and recorded. The sealing pocket is stored in an ice box and transported back to the laboratory for use. After the larvae are brought back to the laboratory, the surface of the body is sterilized for 15 s using 75% ethanol disinfectant solution, followed by washing with sterile water. The disinfection process is repeated three times. The sterilized larvae are placed under a dissecting mirror, the head and tail of the larvae are handed over using ophthalmic surgical scissors, and then the larvae are cut open from the side of the body to remove the intestine. The intestines are placed in a 1.5 mL centrifuge tube, and thoroughly ground after adding 100 μL of phosphate buffer. Dilute 10 times gradient to 10-8, then coat the dilution on the soybean casein medium plate, after 28 ...
Embodiment 2
[0037] Confrontation culture test of Haemobacterium M0055 and Fusarium cereus cucumber specialization
[0038] Prepare the PDA plate for use using a 60 mm Petri dish, punching a hole 0.5 cm from the outer edge of the plate on the edge side of the PDA plate. After inoculating a small amount of Hematobacter M0055 plate culture in soybean casein medium at 30 °C and 180 rpm for 12 h, 25 μL was added to the edge of the PDA plate in one side of the well, and then inoculated with Fusarium cereus cucumber specialized bacterial cake at the opposite side of the inoculation site, and the control used equal volume liquid soybean casein medium instead of the bacterial solution, and 3 parallel controls were set for each bacterial strain, and after 5 days of incubation at 26 °C dark conditions, the growth of the specialized type of Fusarium ceresus cucumber and the inhibition circle were observed. The results showed that Haemobacterium M0055 had a significant inhibitory effect on the specialized...
Embodiment 3
[0039] Example 3 potting experiment
[0040] A small amount of Hemobacter soy casein agar medium culture was inoculated in 1 mL soy casein medium at 30 °C and 180 rpm for 12 h. The above culture was transferred to a triangular bottle containing 150 mL of soybean casein medium at 30 °C and 180 rpm for 72 h; 6 bottles of culture medium were collected 6000 rpm centrifuged for 10 min, the supernatant was discarded, and the mother liquor of Haemin m0055 cell suspension was obtained using 100 mL of resuspensive Haeminum M0055 cells containing 0.05% Tween-80. Subsequently, continue to dilute the mother liquor of the cell suspension of Haeminobacterium M0055 to a concentration of 10 using the above sterile phosphate buffer 6 、10 7 、10 8 、10 9 、10 10 CFU / mL。
[0041] Using the above different concentrations of Heptobacterium cell suspension dilution, soak the roots of a group of 10 cucumber seedlings to be transplanted in the diluted cell suspension for 30 min, drain after removal (until t...
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