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Micro-needle patch as well as preparation method and application thereof

A micro-needle sticker and micro-needle technology, applied in biochemical equipment and methods, microbial determination/inspection, coating, etc., can solve the problems of increasing the difficulty of allergen methods, application limitations, and damage to allergenic proteins, etc. To achieve the effect of convenient follow-up operation, easy operation, and not easy to cause pollution

Active Publication Date: 2021-02-05
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, frying, frying, boiling and other processes in the processing process will also damage the allergenic proteins in the raw materials to varying degrees, thus increasing the difficulty of direct detection of allergens through allergenic proteins
At present, the most common methods for specific detection of allergen DNA in food are PCR and qRT-PCR technology, but before using this detection method, it usually needs to use a variety of lysates to extract DNA, which takes about 4 hours. ~5h, resulting in limited application of this method
Although commercial kits for extracting DNA from deep-processed foods are currently on the market, the whole process still takes 2 to 3 hours. Therefore, research on rapid pretreatment methods has become an important direction to expand the application range of detection technology.

Method used

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  • Micro-needle patch as well as preparation method and application thereof
  • Micro-needle patch as well as preparation method and application thereof
  • Micro-needle patch as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Such as figure 1 and figure 2 As shown, the present embodiment provides a method for preparing a microneedle patch, comprising the following steps:

[0038] (1) Disperse nickel oxide or zinc oxide in MES (2-(N-morpholine)ethanesulfonic acid) buffer solution at 25° C. to prepare a metal oxide solution with a concentration of 0.2 mg / mL.

[0039] (2) Add polyvinyl alcohol (PVA) to ultrapure water at 95°C in a mass ratio of 15wt%, and add 10vol% nickel oxide or zinc oxide solution, and use a magnetic stirrer at 95°C and 1000rpm Stir until the PVA is completely dissolved in water, and stand for a few hours to get a clear, fluid needle preparation solution.

[0040] (3) Put the polydimethylsiloxane (PDMS) microneedle template into a beaker filled with ultrapure water and boil it for 5 minutes, then put it into 75% ethanol solution and clean it with an ultrasonic cleaner for 5 minutes, and place The obtained needle body preparation solution is coated on the cleaned and dri...

Embodiment 2

[0044]This example provides an application of a microneedle patch. The prepared microneedle patch is applied to rapidly extract allergen DNA from homemade milk pudding (agar-based) and perform qualitative detection by PCR.

[0045] Considering the hardness and shape of the milk pudding, this example uses the model of the microneedle template in Table 1, and the mass fraction of the needle body preparation liquid is selected as 13wt%.

[0046] The model of the microneedle template selected in the present embodiment of table 1

[0047]

[0048] Milk pudding preparation process: add agar powder with a content of 0.8wt% to fresh milk, boil and cool to form milk pudding.

[0049] Stick the microneedle patch on the surface of the milk pudding, press it gently with your hands, and transmit the puncture force to the patch, and the swelling and absorption time is 1 minute. The microneedle patch was peeled off and washed several times with 50 μL TE buffer. The resulting solution was...

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Abstract

The invention belongs to the technical field of food allergen detection, and particularly relates to a preparation method of a micro-needle patch, which comprises the following steps of: dispersing ametal oxide in an MES buffer solution to prepare a metal oxide solution; adding polyvinyl alcohol into ultra-pure water, adding the obtained solution into the metal oxide solution, and stirring to obtain a needle body preparation solution; coating a micro-needle template with the needle body preparation liquid, so as to enable the micro-needle template to be filled with the needle body preparationliquid; and performing positive pressure treatment, vacuum treatment and constant-temperature drying on the micro-needle template, and demolding to obtain a micro-needle patch. The micro-needle patchis applied to rapid extraction of DNA, the extraction time can be shortened, the extracted DNA can be directly used for semi-quantitative detection of allergens in highly processed food without purification, the micro-needle patch has the advantages of minimal invasion and short extraction time, the operation is easy and convenient, purification is not needed, and subsequent operation is facilitated.

Description

technical field [0001] The invention belongs to the technical field of food allergen detection, and in particular relates to a microneedle patch and its preparation method and application. Background technique [0002] Food allergy is a common type of health problem in human daily life. So far, the detection methods for food allergens are mainly divided into two types, one is direct detection of allergens for allergenic proteins, and the other is DNA fragments that indirectly indicate the presence of allergen components. Although DNA is easy to degrade, in the food matrix, compared with protein, DNA can remain stable for a long time under heat and pressure treatment conditions, and the degradation rate of target DNA is much slower than protein, so the residual component allergen Characteristic DNA can better indicate the presence of allergens. [0003] Due to the complex food composition, deep-processed food contains not only a variety of raw material components, but also ...

Claims

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Application Information

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IPC IPC(8): C08J7/04C09D129/04C09D7/61C12Q1/6806C08L27/18
CPCC08J7/043C09D129/04C09D7/61C12Q1/6806C08J2327/18C08J2429/04C08K2003/2293C08K2003/2296C08K2003/2241C08K3/22C12Q2523/308
Inventor 王彦波傅玲琳李欢冯洁斯
Owner ZHEJIANG GONGSHANG UNIVERSITY