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A fungal agent prepared by using Trichoderma harzianum ta97 and its application in the prevention and treatment of cropping diseases

A technology of ta97 and inoculum, applied in the field of biological inoculants, can solve the problems of excessive soil pesticide residues, pollution of surrounding water resources, and incompatibility with green environmental protection, etc., and achieve the effect of prolonging the control effect time, improving performance and adhesion performance.

Active Publication Date: 2022-04-15
BIOTECH CENT OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to reduce the threat of cropping diseases to crops, chemical drugs are often used to kill pathogenic bacteria. However, long-term use of chemical drugs has seriously exceeded the standard of pesticide residues in the soil, and chemical drugs have entered groundwater with rainwater, polluting surrounding water resources, which does not meet the requirements of environmental protection. Require

Method used

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  • A fungal agent prepared by using Trichoderma harzianum ta97 and its application in the prevention and treatment of cropping diseases
  • A fungal agent prepared by using Trichoderma harzianum ta97 and its application in the prevention and treatment of cropping diseases
  • A fungal agent prepared by using Trichoderma harzianum ta97 and its application in the prevention and treatment of cropping diseases

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 African Trichoderma harzianum Ta97 measures the antagonism of four kinds of pathogenic bacteria

[0032] (1) Source of pathogenic bacteria

[0033] The pathogenic bacteria were collected from corn-wheat multi-cropping farmland in Jinan City, Shandong Province. According to morphological and molecular identification, Rhizoctonia solani is the pathogen of wheat and corn sheath blight, Alternaria sp. is the pathogen of wheat leaf blight and corn leaf blight, Fusarium oxysporum (Fusarium sp. oxysporum) and Fusarium moniliforme are root rot pathogens of wheat and corn.

[0034] (2) Standoff test

[0035]Introduce Trichoderma harzianum Ta97 and the above four pathogenic bacteria into PDA medium respectively, and activate twice at 25°C. After the second activation and growth for three days, punch the fungus cake from the edge of the colony with a 5mm hole punch; Put the mold Ta97 and Rhizoctonia solani on both ends of the center line of the same 9cm PDA plate, ...

Embodiment 2

[0037] Embodiment 2 Trichoderma harzianum Ta97 Trichoderma conidia powder preparation 1

[0038] The fermentation process of Trichoderma conidia powder is as follows:

[0039] (1) Obtaining the seed solution: Inoculate Trichoderma harzianum Ta97 preserved at -80°C on a PDA solid plate, activate it at 25°C for 3 days, take mycelium from the edge of the colony and inoculate it on the PDA plate again, and cultivate it at 25°C for 3 days. day, take the mycelium at the edge of the colony and culture it at 25°C for 3 days, which is the activated strain; put the activated strain on a PDA plate, and culture it alternately for 10 days at 25°C, 12 hours of light and 12 hours of semi-darkness, and take the spores , to prepare 10 7 conidia suspension per mL, inoculate the conidia suspension in the PDB culture medium, the volume ratio of the conidia suspension to the PDB culture medium is 1:100, and then at 25°C, 180rpm Under shaking culture for 4 days, it is the seed liquid, and the num...

Embodiment 3

[0043] Embodiment 3 Trichoderma harzianum Ta97 Trichoderma conidia powder preparation 2

[0044] (1) Obtaining the seed solution: Inoculate Trichoderma harzianum Ta97 preserved at -80°C on a PDA solid plate, activate it at 25°C for 3 days, take mycelium from the edge of the colony and inoculate it on the PDA plate again, and cultivate it at 25°C for 3 days. day, take the mycelium at the edge of the colony and culture it at 25°C for 3 days, which is the activated strain; put the activated strain on the PDA plate, and culture it alternately for 10 days at 25°C, 12 hours of light and 12 hours of darkness, and take the spores. Prepare 10 7 conidia suspension per mL, inoculate the conidia suspension in the PDB culture medium, the volume ratio of the conidia suspension to the PDB culture medium is 1:100, and then at 25°C, 180rpm Under shaking culture for 5 days, it is the seed liquid, and the number of viable bacteria in the seed liquid is 1×10 8 cfu / mL;

[0045] (2) Obtaining co...

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Abstract

The invention provides a fungal agent prepared by using African Trichoderma harzianum Ta97 and its application in preventing and treating heavy cropping diseases. 7 ~1×10 8 Cfu / mL seed solution was inoculated into solid fermentation medium, stirred evenly, cultured for 5-8 days at a constant temperature of 25-28°C, 12 hours of light and 12 hours of darkness, air-dried and passed through 60 and 100 mesh sieves successively, and taken Mix the under-sieve part with diatomaceous earth according to the weight ratio of 5 to 7:1 to obtain Trichoderma conidia powder; mix the Trichoderma conidia powder with an auxiliary agent to obtain a bacterial agent; use the bacterial agent to prevent heavy cropping Disease application. The control effect of the bacterial agent on the repeated cropping disease is more than 80%, which shows that the bacterial agent has a good control effect on the repeated cropping disease, and is beneficial to continuous cropping in the field.

Description

technical field [0001] The invention relates to the technical field of biological inoculum, in particular to an inoculum prepared by using African Trichoderma harzianum Ta97 and its application in preventing and treating heavy cropping diseases. Background technique [0002] Continuous cropping is also called heavy cropping, which is a planting method for rational use of land, among which corn-wheat multiple cropping is one of the typical continuous cropping modes. Although this planting method maximizes the use of land, since the crops carry plant pathogens during the growth process, the pathogens will gradually accumulate in the soil during the continuous cropping process, causing repeated cropping diseases and seriously endangering agricultural production safety. [0003] The disease of heavy cropping mainly comes from the pathogenic bacteria parasitic in the aboveground part and root system of the crops. The pathogenic bacteria in the aboveground part enter the soil with...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/38A01N25/14A01P3/00C09K17/14A01B79/02A01G13/00C09K101/00C12R1/885
CPCC12N1/14A01N63/38A01N25/14C09K17/14A01B79/02A01G13/00C09K2101/00C12R2001/885C12N1/145
Inventor 吴晓青张新建周方园周红姿赵晓燕谢雪迎王加宁
Owner BIOTECH CENT OF SHANDONG ACAD OF SCI
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