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Hemostatic sealing agent and preparation method thereof

A technology of sealing agent and thrombin, which is applied in the field of hemostasis, can solve the problems of poor stability, easy fire, short storage time, etc., and achieve the effects of ensuring safety, improving storage stability, and excellent hemostatic effect

Pending Publication Date: 2021-02-12
广东深蓝生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, fibrinogen and thrombin are easy to catch fire after leaving the body, and the storage time is short and the stability is poor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Hemostatic sealant

[0038] The above-mentioned hemostatic sealing agent includes fibrinogen solution and thrombin solution;

[0039] Each kilogram of fibrin solution includes the following components:

[0040] Fibrinogen 2g, sodium chloride 6g, sodium citrate 10g, sucrose 5g, Tris 3g and lysine hydrochloride 5g;

[0041] Each kilogram of thrombin solution includes the following components:

[0042] Thrombin 6.1g, sodium chloride 5g, Tris 6g, sodium acetate trihydrate 4g and glacial acetic acid 0.6ml;

[0043] The pH value of the first suspension buffer is 7.1; the pH value of the second suspension buffer is 5.9.

[0044] 2. Preparation method

[0045] The preparation method of above-mentioned hemostatic sealing agent, this preparation method comprises the steps:

[0046] (a) Use 0.05M acetic acid-sodium acetate buffer solution containing 0.1M sodium chloride and pH5.8 to dilute the pig plasma in equal times (i.e. equal volume) and lower the temperature, add 0.5...

Embodiment 2

[0054] 1. Hemostatic sealant

[0055] The above-mentioned hemostatic sealing agent includes fibrinogen solution and thrombin solution;

[0056] Each kilogram of fibrin solution includes the following components:

[0057] Fibrinogen 8g, sodium chloride 9g, sodium citrate 6g, sucrose 8g, Tris 1g and lysine hydrochloride 8g;

[0058] Each kilogram of thrombin solution includes the following components:

[0059] Thrombin 2.7, sodium chloride 10g, Tris 3g, sodium acetate trihydrate 8g and glacial acetic acid 0.2ml;

[0060] The pH value of the first suspension buffer is 7.3; the pH value of the second suspension buffer is 4.9.

[0061] 2. Preparation method

[0062] The preparation method of above-mentioned hemostatic sealing agent, this preparation method comprises the steps:

[0063] (a) Use 0.05M acetic acid-sodium acetate buffer solution containing 0.1M sodium chloride and pH5.8 to dilute the pig plasma in equal times (i.e. equal volume) and lower the temperature, add 0.5 ...

Embodiment 3

[0071] 1. Hemostatic sealant

[0072] The above-mentioned hemostatic sealing agent includes fibrinogen solution and thrombin solution;

[0073] Each kilogram of fibrin solution includes the following components:

[0074] Fibrinogen 6g, sodium chloride 8g, sodium citrate 8g, sucrose 6g, Tris 2g and lysine hydrochloride 7g;

[0075] Each kilogram of thrombin solution includes the following components:

[0076] Thrombin 5.7g, sodium chloride 8g, Tris 5g, sodium acetate trihydrate 6g and glacial acetic acid 0.4ml;

[0077] The pH value of the first suspension buffer is 7.2; the pH value of the second suspension buffer is 5.4.

[0078] 2. Preparation method

[0079] The preparation method of above-mentioned hemostatic sealing agent, this preparation method comprises the steps:

[0080] (a) Use 0.05M acetic acid-sodium acetate buffer solution containing 0.1M sodium chloride and pH5.8 to dilute the pig plasma in equal times (i.e. equal volume) and lower the temperature, add 0.5 ...

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Abstract

The invention discloses a hemostatic sealing agent and a preparation method thereof. The hemostatic sealing agent comprises a fibrinogen solution and a thrombin solution; each kilogram of the fibrin solution comprises the following components: 2-8g of fibrinogen, 6-9g of sodium chloride, 6-10g of sodium citrate, 5-8g of cane sugar, 1-3g of Tris and 5-8g of lysine hydrochloride; each kilogram of the thrombin solution comprises the following components: 2-8g of thrombin, 5-10g of sodium chloride, 3-6g of Tris, 4-8g of sodium acetate trihydrate and 0.2-0.6 ml of glacial acetic acid; the sealing agent consists of a fibrinogen solution and a thrombin solution, and has an excellent hemostatic effect; moreover, the fibrinogen and the thrombin are respectively stored by adopting specific solutions, so that the storage stability of the fibrinogen and the thrombin can be improved, the storage time is long, and excellent biological activity is maintained.

Description

technical field [0001] The invention relates to the technical field of hemostasis, in particular to a hemostatic sealing agent and a preparation method thereof. Background technique [0002] Fibrinogen is an active protein component in both animal and human plasma. Before the 1970s, some developed countries such as the United States, Austria and Germany had produced human fibrinogen on a large scale, and its curative effect has been affirmed. , and the demand is also increasing year by year. However, due to the lack of effective virus inactivation steps in the preparation process of human fibrinogen at that time, it became a source of pollution for virus transmission. Later, various countries gradually banned production and sales. After the 1970s, with the maturity of S / D virus inactivation methods, coagulation factor products such as fibrinogen, coagulation factor VIII, prothrombin complex and other products resumed production. The fibrinogen derived from porcine plasma ha...

Claims

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Application Information

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IPC IPC(8): A61L24/10A61L24/00
CPCA61L24/001A61L24/106A61L24/108A61L2400/04C08L89/00
Inventor 吕应年林善子
Owner 广东深蓝生物科技有限公司
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