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Use of total saponins of panax japonicas in preparing medicine for treating rheumatoid arthritis angiogenesis

A technology for total saponins and angiogenesis of Panax ginseng, which can be applied in drug combinations, plant/algae/fungus/moss components, antipyretics, etc., can solve the structural disorder of new blood vessels, cannot change the hypoxic state of synovium, and can not function fully. And other issues

Inactive Publication Date: 2021-02-26
CHINA THREE GORGES UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Under hypoxic conditions, synovial blood supply and oxygen supply are insufficient, and synovial blood supply and oxygen deficiency promote synovial tissue angiogenesis to meet the demand for oxygen and nutrition of synovial tissue, but the structure of new blood vessels is disordered, incomplete, and can not change the state of synovial hypoxia

Method used

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  • Use of total saponins of panax japonicas in preparing medicine for treating rheumatoid arthritis angiogenesis
  • Use of total saponins of panax japonicas in preparing medicine for treating rheumatoid arthritis angiogenesis
  • Use of total saponins of panax japonicas in preparing medicine for treating rheumatoid arthritis angiogenesis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: Get the bamboo ginseng medicinal material, accurately weighed, 10 times the amount of 70% ethanol heating and reflux extraction 3 times, each time for 2 hours, 80 ° C water bath concentration, filtering, merging the filtrate, and the filtrate concentrating under reduced pressure to obtain the bamboo joint Ginseng active ingredient.

Embodiment 2

[0027] Embodiment 2: HPLC method detects the content of four kinds of active components in the total saponins of Panax ginseng

[0028] Experimental method: In this experiment, the HPLC method was used to detect whether the total saponins of Panax ginseng contained four active ingredients. The test results showed that it contained ginsenoside Rg2, ginsenoside Ro, Panax ginseng saponin V, and Panax ginseng saponin IVa.

[0029] The experimental system is: chromatographic column Welch Ultimate XB-C18 150×4.6 mm, 5 μm, column temperature 30°C; DAD detector, detection wavelength is 200 nm; mobile phase A is 0.1% phosphoric acid aqueous solution, B is acetonitrile, using the following gradient Elution; flow rate is 1.0 mL / min; injection volume is 10 μL.

[0030] The mobile phase gradient is shown in the table:

[0031]

[0032] See the experimental results figure 1 .

Embodiment 3

[0033] Example 3: Total saponins of Panax japonicus (TSPJ) inhibit angiogenesis in mice with arthritis through the HIF-1α-VEGF-Ang-1 pathway

[0034] Experimental method: 60 male DBA / 1J mice, weighing 16-18g, after one week of adaptive feeding, 8 were randomly selected as the normal group (hereinafter referred to as group C), and the rest of the mice were used as the model group. Dissolve in 10mM acetic acid according to CⅡ, the concentration is 2mg / mL, stir, and fully dissolve overnight at 4°C. Take an equal volume of CII and mix it into a 5mL glass syringe, pump it repeatedly for 30 minutes (operated on ice), until the mixture is completely and fully emulsified, and the emulsion is not loose when it is dripped into the water, and the antigen is made. The mice in the model group were injected with 0.1 mL of antigen on the left side of the base of the tail, and the mice in the normal group were given 0.1 mL of PBS. 14 days after the initial immunization, CII was mixed with an...

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Abstract

The invention provides use of total saponins of panax japonicas in preparing a medicine for treating rheumatoid arthritis angiogenesis. Preferably, the medicine for relieving the rheumatoid arthritisangiogenesis takes effects through an HIF-1alpha / VEGF / ANG-1 signal pathway by the total saponins of panax japonicas. Results show that compared with a normal group, an thymus index, a spleen index, alymph node index, a posterior foot claw thickness and the foot swelling number of mice in a model group are obviously increased; a morphology shows obvious inflammatory cell infiltration, vascular proliferation and cartilage and bone destruction; an expression of an angiogenesis-related gene CD31 is increased and the number of immature blood vessels is obviously increased; and the contents of HIF-1alpha, VEGF and ANG-1 in serum are increased, and mRNA levels and protein expression levels of the HIF-1alpha, VEGF and ANG-1 are increased. After treatment by the total saponins of panax, the thymusindex, spleen index, lymph node index, foot posterior claw thickness and foot swelling number are obviously reduced; and the morphological inflammatory cell infiltration, vascular proliferation, cartilage and bone destruction are reduced.

Description

technical field [0001] The invention relates to a pharmaceutical use of total bamboo ginseng saponins, in particular to the application of total bamboo ginseng saponins in the preparation of medicines for treating rheumatoid arthritis. It belongs to the technical field of biopharmaceuticals. Background technique [0002] Rheumatoid arthritis (rheumatoid arthritis, RA) is a chronic autoimmune disease of unknown etiology. Prone to hair, the incidence rate is 2 to 3 times that of men. The prevalence rate of RA patients in China is about 0.16%. The main pathological changes are chronic synovitis, synovial hyperplasia, pannus formation and final cartilage and bone destruction. [0003] Under hypoxic conditions, synovial blood supply and oxygen supply are insufficient, and synovial blood supply and oxygen deficiency promote synovial tissue angiogenesis to meet the demand for oxygen and nutrition of synovial tissue, but the structure of new blood vessels is disordered, incomplete...

Claims

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Application Information

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IPC IPC(8): A61K36/258A61P19/02A61P29/00
CPCA61K36/258A61P19/02A61P29/00
Inventor 冯知涛梅志刚付先芸吉金雨郭响罗亚男
Owner CHINA THREE GORGES UNIV
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