Preparation method and application of glycopeptide-enriched magnetic polyaniline nano material

A nanomaterial and magnetic nanotechnology, applied in the field of preparation of magnetic polyaniline nanomaterials, achieves the effects of mild enrichment conditions, high anti-interference ability and good enrichment effect

Active Publication Date: 2021-03-05
THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the high dynamic range of glycoproteins, the inherently low glycopeptide abundance, and the severe ion suppression caused by the coexistence of non-glycosylated peptides make direct mass spectrometric analysis of glycopeptides / glycopeptides a challenge

Method used

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  • Preparation method and application of glycopeptide-enriched magnetic polyaniline nano material
  • Preparation method and application of glycopeptide-enriched magnetic polyaniline nano material
  • Preparation method and application of glycopeptide-enriched magnetic polyaniline nano material

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Effect test

Embodiment 1

[0056] Enrichment of Glycopeptides Using Magnetic Polyaniline Nanomaterials

[0057] 1. Preparation of magnetic polyaniline nanomaterials

[0058] (1) Weigh 1.35g of ferric trichloride hexahydrate in a beaker, measure 75mL of ethylene glycol solution and stir to dissolve for 0.5 hours. After stirring to a yellow solution, add 3.6 g of anhydrous sodium acetate anhydrous sodium acetate anhydrous sodium acetate powder, and continue stirring for 1 hour. Pour the resulting solution into the reaction kettle and not exceed 2 / 3 of the total volume of the reaction kettle, heat it in an oven at 200°C for 16 hours, then transfer the product to a beaker and use absolute ethanol, 50% ethanol, ultrapure water and volume Fractions of 80% acetonitrile aqueous solution and acetonitrile were shaken and washed, and dried in an oven at 60°C to obtain magnetic nanomaterials.

[0059] (2) Add 0.3 mL of aniline solution to 30 mL of 0.1% aqueous hydrochloric acid solution, and place in an ice bath ...

Embodiment 2

[0106] Example 2 Morphology and structural characterization of magnetic polyaniline nanomaterial-enriched materials

[0107] In this example, the morphology and structure of the material enriched with magnetic polyaniline nanomaterials were characterized, and the mechanism of enrichment of glycopeptides with magnetic polyaniline nanomaterials was further speculated.

[0108] Figure 6 : Transmission electron microscope images of magnetic beads (a and b) and magnetic polyaniline nanomaterials (c and d), it can be found that both magnetic beads and magnetic polyaniline nanomaterials are spherical structures with a size of about 500nm, and the size is relatively uniform. Uniformly coated on the surface of magnetic beads.

[0109] Figure 7 : XPS reveals the elemental composition of magnetic polyaniline nanomaterials. C was observed at 282.19, 397, 529.97 and 708.73 eV 1s , N 1s , O 1s and Fe 2p The peaks indicate that the material is mainly composed of C, N, O and Fe eleme...

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Abstract

The invention discloses a preparation method and application of a glycopeptide-enriched magnetic polyaniline nano material, wherein the magnetic polyaniline nano material is prepared by firstly heating ferric trichloride hexahydrate, ethylene glycol and anhydrous sodium acetate at high temperature to obtain a magnetic nano material, and then coating polyaniline on the surface of the magnetic nanomaterial. The glycopeptide enrichment method comprises the following steps: adding a magnetic polyaniline nano-material dispersion liquid into a glycoprotein pancreatin enzyme digestion product, and carrying out oscillation enrichment; and after the enrichment liquid is subjected to magnetic separation, adding eluent into the glycopeptide-enriched magnetic polyaniline nanometer material for oscillation elution, and then carrying out magnetic separation to obtain a glycopeptide enrichment solution. According to the material and the method, richer protein glycosylation modification information and more accurate glycan glycoform can be obtained, low-cost, environment-friendly and high-specificity enrichment and separation are achieved under the condition that the protein glycosylation modification structure is not damaged, operation is easy and convenient, and a novel material and a technical method are provided for protein glycosylation modification research in a complex sample, screening of related tumor markers and the like.

Description

technical field [0001] The invention relates to the technical field of protein and polypeptide enrichment, in particular to a preparation method and application of a magnetic polyaniline nanometer material enriched with glycopeptides. Background technique [0002] Protein glycosylation is one of the most important post-translational modifications, which plays important structural and functional roles in various biological processes such as cell-cell interaction, immune defense, cell growth and differentiation. Aberrant glycosylation on some important proteins is highly associated with various diseases such as cancer and neuromuscular diseases. N-glycosylated proteins are also important for disease prognosis, diagnosis, and drug treatment response. Therefore, protein glycosylation analysis is of great significance not only to biomedical research, but also to the biotechnology industry. Currently, mass spectrometry (MS)-based techniques have become the most important and pow...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/34G01N1/38G01N1/40C08G73/02
CPCG01N1/34G01N1/38G01N1/40C08G73/0266
Inventor 李智立赖治臻张沫
Owner THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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