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Synbiotics and feed for improving intestinal health and body immunity of turbots

A technology for intestinal health and turbot, applied in animal feed, animal feed, bacteria used in food preparation, etc., can solve problems such as failure to achieve expected results, waste of resources, and lack of scientific basis for compatibility, and achieve close improvement. The effect of linking barrier function, quality assurance, and improving the level of immune response in fish

Active Publication Date: 2021-03-26
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For biostime products, many product combinations do not have a scientific basis, not only failing to achieve the desired effect, but also causing a waste of resources

Method used

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  • Synbiotics and feed for improving intestinal health and body immunity of turbots
  • Synbiotics and feed for improving intestinal health and body immunity of turbots
  • Synbiotics and feed for improving intestinal health and body immunity of turbots

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] First, the protective effect of water sugar on the intestinal health of Daxumi in the food, high-level cotton meal is evaluated by breeding experiment, determining the appropriate amount of water sugar in the feed, and combines high-throughput sequencing analysis of intestinal flora As a result, probiotic strains suitable for mixing with water sugar were screened.

[0028] The specific method of operation is as follows: 4 of the four kinds of nitrogen-free feed, the crude protein content is 52%, the crude fat content is 12%. The fish powder group is the main protein source with fish powder, replacing 40% fish powder protein with cotton meal, adding water sugar in the cotton rice group, adding water sugar to a 1.4% water sugar group and 2.8% water sugar. group. The water sugar (purity ≥ 80.0%) used in this experiment was purchased from China Food and Fermentation Industry Research Institute. The actual content of water sugar in four experimental feed formulations was 0.13% (...

Embodiment 2

[0059] Example 2 Preparation of Taling Combined Evasion:

[0060] After mixing 2.5% water sugar with other raw materials, the hard particle feed was made after extrusion drying. The strains were cultured overnight in MRS medium, and 1 ml of bacterial liquid was centrifuged at 3500 g of centrifuge, and the PBS was resuspended twice. The 标 仪 仪 仪 仪 曲 曲 曲 曲 曲, 仪 仪 仪, According to 1 × 10 8 The concentration of CFU / g sprayed it into a water sugar-containing feed and completely mixed well.

[0061] The water sugar is purchased in China National Food Fermentation Industrial Research Institute Co., Ltd., which is extracted from grass silkworm, purity ≥ 80%.

[0062] The cheese lactobacter is purchased in the China Industrial Microbiology Culture Collection (CICC), and the strain is available in CICC 6117.

Embodiment 3

[0063] Example 3 Synbiotics to improve the application of Immune response capabilities and intestinal health

[0064] 2.5% water sugar added in the basic feed, add 1 × 10 8 CFU / G Cheese Lactobacillus and simultaneous addition of 2.5% water sugar and 1 × 10 8 CFU / G Cheese Lactobacillus (Hym Group), the control group did not add water sugar and cheese Lactobacillus base feed, 4 sets of feed, such as nitrogen, etc., where water sugar and control group were added. The formula is added in Table 5, and the cheese Lactobacterium is added in the basic feed and aqua-sugar feed (the two groups are not listed). Feed raw materials are ultra-microclinated through 60 mesh sieve, each raw material is mixed and uniform, and a 3 mm particle diameter feed is extruded. The dried in a venting oven at 55 ° C is deposited. -20 ° C refrigerator stocks. According to 1 × 10 8The concentration of CFU / g was sprayed with a wrap-resusted cheese lactacillus to a water sugar-containing feed. After sprayin...

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Abstract

The invention relates to synbiotics for improving intestinal health and body immunity of turbots and a feed, belongs to the technical field of probiotic additives for aquaculture feed, and the synbiotics are composed of stachyose and lactobacillus casei. The invention also provides a turbot feed containing the synbiotics, the mass ratio of stachyose in the turbot feed is 2.0-3.0%, and the concentration of the lactobacillus casei in the synbiotic in the turbot feed is 1 * 10 <7> - 1* 10 <9> CFU / g. The feed can significantly improve abundance of a specific probiotic strain lactobacillus casei inintestine, improve intestinal morphology and structural integrity, and improve immune response capability of turbots, thereby improving intestinal health and disease resistance of cultured turbots.

Description

Technical field [0001] The present invention belongs to the technical field of aquaculture feed additive, and in particular to syndrome additives and feed to promoting the intestinal health and immune response levels of Daxum, which are compatible with lactobacilluscasei. . Background technique [0002] In recent years, aquaculture has increased significantly on the contribution of food production in the world, and the advancement of breeding technology has stimulated the rapid development of aquaculture industry. However, due to the restrictions on the aquaculture resources such as water, high-density culture remains an inevitable trend. In addition, because of the extreme tension of fish powder resources, the source is stable, and the price of low price is widely used in the aquaculture feed, and it is often easily caused by aquaculture fish in the application, especially the kind of meat-raised fish. The intestines are important digestion and absorption organs, and also have i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/163A23K10/18A23K50/80
CPCA23K20/163A23K10/18A23K50/80A23V2400/169Y02A40/818
Inventor 张彦娇戴济鸿张文兵艾庆辉麦康森
Owner OCEAN UNIV OF CHINA
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