Aluminum ion fluorescent probe and preparation method and application thereof
A technology of fluorescent probes and aluminum ions, which is applied in the field of fluorescent probe detection, can solve problems such as complex preparation processes, and achieve the effects of simple synthesis methods, high yields, and good specificity
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Embodiment 1
[0025] see figure 1 It is a product schematic diagram of the aluminum ion fluorescent probe prepared in the present invention, and the molecular formula of the aluminum ion fluorescent probe is C 16 h 12 N 2 o 2 S 1 ; The structural formula of the aluminum ion fluorescent probe is:
[0026]
[0027] The invention also discloses a method for preparing an aluminum ion fluorescent probe, which specifically includes the following steps:
[0028] (1) Under heating and stirring conditions, 2-thienyl hydrazide (142 mg, 1 mmol) and 2-hydroxy-1-naphthaldehyde (172 mg, 1 mmol) were dissolved in ethanol (10 mL);
[0029] (2) Slowly add the 2-hydroxy-1-naphthaldehyde solution dissolved in ethanol dropwise to the 2-thienyl hydrazide solution dissolved in ethanol, add 200 μL of catalyst glacial acetic acid, condense and reflux for 3-3.5 hours;
[0030] (3) After the reaction is completed, cool to obtain pale yellow needle-like crystals, filter and wash the crystals with ethanol, dr...
Embodiment 2
[0036] This example provides a test of the aluminum ion detection performance using the aluminum ion fluorescent probe prepared in Example 1: first prepare a fluorescent probe dimethyl sulfoxide (DMSO) stock solution with a concentration of 2 mM and different metals with a concentration of 4 mM Ions (Na + , Mg 2+ 、K + , Ga 2+ , Zn 2+ 、Al 3+ , Fe 3+ and Cu 2+ ) deionized water stock solution; then use it to configure 1mL fluorescent probe concentration of 20 μM and different metal ion concentrations of 40 μM DMSO solution, deionized aqueous solution, and observe and take pictures under ultraviolet light, as Figure 4 As shown, among them, Figure 4 A is DMSO, deionized water, Na + , Mg 2+ 、K + , Ga 2+ , Zn 2+ 、Al 3+ , Fe 3+ and Cu 2+ , the ultraviolet light excitation pictures of DMSO solution at a wavelength of 365nm arranged from left to right; Figure 4 B is DMSO, deionized water, Na + , Mg 2+ 、K + , Ga 2+ , Zn 2+ 、Al 3+ , Fe 3+ and Cu 2+ , from left to...
Embodiment 3
[0039] This example is a study on the cytocompatibility of the aluminum ion fluorescent probe prepared according to Example 1; the fluorescent probe with a gradient concentration (0-60 μmol / L, with an interval of 10 μM) was co-cultured with Hela cells for 1 day, and tested OD value of cellular protein concentration, from Figure 6 It can be seen that the cytocompatibility of the fluorescent probe is good, and the survival rate is higher than 95%. Therefore, the aluminum ion fluorescent probe prepared in the present invention can be used for intracellular fluorescence imaging to detect the concentration of aluminum ion.
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