Preparation method and use method of plant protective agent

A technology of plant protection agent and yeast extract, which is applied in botany equipment and methods, plant protection, plant growth regulator, etc., and can solve problems such as poor effect, loss of tomato farmers, invasion and infection, etc.

Inactive Publication Date: 2021-04-06
阳田生物科技(江苏)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cross-cut fresh diseased stems, squeeze them with your hands or insert them into clear water, and milky white bacteria can be seen overflowing, which is the main feature of the disease The flow of shoes, agricultural machinery, and irrigation water also have the opportunity to carry pathogenic bacteria for transmission, and pathogenic bacteria can survive in the soil for several years for a long time. Ralstonia solanacearum can be colonized in the soil through natural openings in the roots or due to transplantation, Invasion and infection of wounds caused by nematodes, insects, or other physical damage, and if the knife carries R. so...

Method used

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Examples

Experimental program
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Effect test

specific Embodiment 1

[0015] Specific embodiment one, a kind of preparation method of plant protection agent is characterized in that its steps are:

[0016] (1) Streptomyces lydida was first cultured in a liquid shaker flask with 10% (weight percent) of sucrose, 5% (weight percent) of yeast extract, 0.15% (weight percent) of magnesium sulfate, and 0.3% potassium dihydrogen phosphate (percentage by weight) and 0.02% (percentage by weight) of manganese chloride are liquid medium, and in a constant temperature environment of 30 degrees Celsius, the rotating speed is 150 revolutions per minute, and the shaking flask is cultivated for 7 days, so that the concentration of vegetative cells per milliliter of Streptomyces lydidi is up to More than 10^7 cfu / ml;

[0017] (2) Before carrying out solid fermentation, the materials and pots must be sterilized first to ensure that they are in a sterile state. Then, in a sterile state, wash the rice and steam it for more than 1 hour until it is cooked and soft. P...

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PUM

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Abstract

The invention relates to a preparation method and a use method of a plant protective agent, and belongs to the technical field of plant protective reagents. The plant protective agent is prepared by taking streptomyces lydicus, cane sugar, yeast extract, magnesium sulfate, monopotassium phosphate and manganese chloride as main raw materials. The streptomyces lydicus plant protective agent is prepared by taking streptomyces lydicus strain fermentation liquor, confirming that the nutrient cell concentration of each milliliter of the fermentation liquor is over 10<^>7 cfu/ml, inoculating the fermentation liquor to cooked rice serving as a main carrier, carrying out constant-temperature culture for 48 hours, and drying moisture to be below 10 percent at the low temperature of 40 DEG C to obtain the streptomyces lydicus plant protective agent. The preparation method of the plant protective agent is scientific and reasonable in process design, low in preparation cost, simple in process, easy to control and suitable for popularization and application.

Description

technical field [0001] The invention relates to the preparation and application method of a plant protection agent, which belongs to the technical field of plant protection agents. Background technique [0002] Due to the increasing severity of soil-borne diseases in recent years, bacterial wilt is the most serious on Solanaceae such as tomatoes. Bacterial disease invades from root or stem base wounds, and spreads in the vascular tissue of the plant, causing blockage of vascular bundles, inability to transport nutrients, cell poisoning and plant withering and death. Because the pathogenic bacteria can hide in the soil for several years, even the use of pesticides cannot effectively kill the pathogenic bacteria, and excessive use of pesticides will cause harm to the environment and one's own health. From the perspective of bacteria, the fundamental solution is to inhibit the growth of pathogenic bacteria and prevent them from causing harm to crops through prevention rather t...

Claims

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Application Information

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IPC IPC(8): A01N63/28A01P1/00A01G7/06A01G13/00
CPCA01G7/06A01G13/00A01N63/00
Inventor 周廷霖
Owner 阳田生物科技(江苏)有限公司
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