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A composite fluorescent probe for detecting alkaline phosphatase and its preparation method and application

A composite fluorescent and fluorescent probe technology, applied in the field of fluorescent analysis, can solve the problems of poor biocompatibility, cumbersome process, and low safety of sulfur quantum dots, and achieve good biocompatibility, simple process, and low preparation requirements.

Active Publication Date: 2022-05-31
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The process is cumbersome, the safety is low, and the biocompatibility of the prepared sulfur quantum dots is poor

Method used

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  • A composite fluorescent probe for detecting alkaline phosphatase and its preparation method and application
  • A composite fluorescent probe for detecting alkaline phosphatase and its preparation method and application
  • A composite fluorescent probe for detecting alkaline phosphatase and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] (4) The yellow fluorescent sulfur quantum dots obtained in step (3) are freeze-dried to obtain the target fluorescent sulfur quantum dots. its fluorescence

[0062] The preparation of all solutions in the experimental process is solvent with deionized water.

Embodiment 2

[0075] (4) The yellow fluorescent sulfur quantum dots obtained in step (3) are freeze-dried to obtain the target fluorescent sulfur quantum dots. its fluorescence

[0081] The preparation of all solutions in the experimental process is solvent with deionized water.

Embodiment 3

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Abstract

The invention discloses a composite fluorescent probe for detecting alkaline phosphatase, a preparation method and application thereof, and belongs to the technical field of fluorescence analysis. Fluorescent probe of the present invention is sulfur quantum dot-nanometer MnO 2 ‑L‑Ascorbic acid‑2‑phosphate trisodium salt composite fluorescent probe. In the present invention, sulfur quantum dot-MnO 2 The nanocomposite system-L-ascorbic acid-2-phosphate trisodium salt is mixed with different concentrations of alkaline phosphatase standard solutions, and the standard working curve of alkaline phosphatase is obtained by the fluorescence method, and a fluorescent method for detecting alkaline phosphatase is constructed. Sensor, thus establishing a new method for rapid detection of alkaline phosphatase based on the change of fluorescence signal of sulfur quantum dots. The detection method of the invention has the advantages of simplicity, rapidity, reliability, low cost, etc., and can realize the detection of alkaline phosphatase in actual samples.

Description

A composite fluorescent probe for detecting alkaline phosphatase and its preparation method and application technical field The invention belongs to the technical field of fluorescence analysis, be specifically related to a kind of composite fluorescent probe detecting alkaline phosphatase and Its preparation method and application. Background technique Alkaline phosphatase is widely distributed in tissues such as human liver, bone, intestine, kidney and placenta, and is excreted through the liver to the bile. of an enzyme. This enzyme catalyzes the removal of the 5'-phosphate group from nucleic acid molecules, thereby converting the 5'-P terminus of DNA or RNA fragments into a 5'-OH terminus. It directly participates in phosphorus metabolism and plays an important role in the digestion, absorption, secretion and ossification of calcium and phosphorus. effect. As one of the routine clinical testing items, alkaline phosphatase is mainly used to diagnose liver and s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/02C09K11/56C12Q1/42B82Y20/00B82Y30/00B82Y40/00
CPCC09K11/025C09K11/56C12Q1/42B82Y20/00B82Y30/00B82Y40/00
Inventor 卫艳丽周羊羊卫宇馨王丽董川
Owner SHANXI UNIV
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