Active protein peptide gene with excellent repairing effect and encoded protein peptide

Abstract

The invention provides an active protein peptide gene with an excellent repairing effect. The effective nucleotide sequence of the gene is shown as SEQ ID NO: 1; and the invention further provides an active protein peptide (EL for short) coded by the active protein peptide gene with an excellent repairing function. The amino acid sequence of the active protein peptide is shown as SEQ ID NO: 2. According to the invention, through an in-vitro gene protein recombination technology, a gene segment of the active protein peptide is grafted to a genetic information carrier and transplanted to a genetically engineered bacterium escherichia coli DH5a strain, the active peptide protein is prepared through density fermentation, protein separation and purification and freeze drying, and it can be ensured that the activity of the active peptide protein is not reduced for 3 years in a freeze-dried state. Cell experiments and human body clinical test experiments prove that the protein peptide has excellent effects on pox muscle repair and cosmetic medicine postoperation repair. The active peptide is relatively high in safety, and the safety is effectively authenticated through microorganism detection, heavy metal content detection and cell experiments; and the process is verified to be feasible, and batch production can be carried out.

Description

technical field [0001] The present invention relates to an active peptide suitable for the field of cosmetics, in particular to an active protein peptide gene with excellent repairing effect and its encoded protein peptide and its preparation. Background technique [0002] Well-known epidermal growth factor (Epidermal Growth Factor) is called for short EGF, is a kind of important cell growth factor of human endocrine, has very strong physiological activity. EGF was discovered by American scientist Dr. Stanley Cohen in 1986. EGF is a growth factor that exists in the human body, and its main function is to promote the division of skin cells. Studies have shown that a very small amount of EGF can strongly stimulate cell growth, inhibit the expression of aging genes, prevent skin aging, and keep the various components of the skin in the best physiological state. In addition, it can stimulate the synthesis and secretion of some extracellular macromolecules (such as hyaluronic a...

AI Technical Summary

Problems solved by technology

However, EGF has not been widely used in the fields of medicine and cosmetics. The reason is that EGF is difficult to achieve mass production: At present, EGF extraction methods are mainly divided into two types, one is animal sources, such as mouse jaw, bovine brain, etc. The other is to extract from the culture supernatant of stem cells. The content of EGF obtained by the two methods is very small, and the cost is extremely high

In addition, the molecular weight of the usual EGF is about 6000 Daltons, the molecular weight is relatively large, and the penetrating power to the skin is relatively poor, and its efficacy is difficult to give full play to.

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