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Lactobacillus mucoase for relieving endotoxin infection and application

A technology of Lactobacillus mucosae and endotoxin, applied in the field of microorganisms, can solve the problems of insignificant curative effect, inability to effectively remove endotoxin, insufficient clinical data, etc., and achieve the effect of increasing the content of short-chain fatty acids

Active Publication Date: 2021-04-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although traditional Chinese medicine has a certain scavenging effect on endotoxin, the curative effect is not significant; antibiotics cannot effectively remove endotoxin, but have a certain negative effect; blood purification method has a good scavenging effect on endotoxin, but the cost High, and insufficient clinical data

Method used

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  • Lactobacillus mucoase for relieving endotoxin infection and application
  • Lactobacillus mucoase for relieving endotoxin infection and application
  • Lactobacillus mucoase for relieving endotoxin infection and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Screening, identification, cultivation and preservation of mucosal lactobacillus CCFM1142

[0049] 1. Screening

[0050]Take 1g of healthy human feces samples from Mayang Miao Autonomous County, Huaihua City, Hunan Province, apply them on MRS solid medium after gradient dilution, and culture them in an anaerobic environment at 37°C for 72 hours, observe and record the colony morphology; , Protruding, white and yellowish colonies were streaked on the MRS solid medium, and purified and cultivated under anaerobic conditions at 37°C. Repeat this operation 3 times to obtain purified single colonies; pick a single colony on Stretch on the MRS solid medium, culture anaerobically at 37°C for 36 hours, perform Gram staining on the obtained colonies, record the morphology of the colonies, and investigate the physiological and biochemical characteristics of the strains according to the "Common Bacterial System Identification Manual", and retain Gram-negative , the colo...

Embodiment 2

[0065] Embodiment 2: Preparation of Lactobacillus mucous membrane CCFM1142 bacterium liquid

[0066] (1) Dip the bacterial solution of Lactobacillus mucous membranes CCFM1142 from the glycerol tube, streak it on the MRS solid medium, and culture it at 37°C for 48 hours under anaerobic environment to obtain a single colony; pick a single colony and inoculate it in the MRS liquid medium, In an anaerobic environment, culture at 37° C. for 48 hours for activation culture, and repeat this operation 3 times to obtain the activated bacterial liquid.

[0067] (2) Inoculate the activated bacterial liquid obtained in step (1) into the MRS liquid medium according to the inoculum size of 2% (v / v), cultivate at 37°C for 24 hours to obtain a fermentation liquid, and collect the bacteria by centrifugation of the fermentation liquid body, resuspend the cell body with physiological saline, and adjust the number of viable cells to 5×10 9 CFU / mL, made into bacterial suspension.

Embodiment 3

[0068] Example 3: Effect of Lactobacillus CCFM1142 on LPS Infection of LPS in Mouse Serum LPS

[0069] Fifty-six 6-week-old, 18-20g C57BL / 6J male mice were randomly divided into 4 groups after a one-week adaptation period: control group, LPS model group, CCFM1142 group and FZJTZ26M3 group, with 8 mice in each group. Among them, the control group was intraperitoneally injected with 0.2mL of normal saline and gavaged with 0.2mL of protective agent every day (the preparation method of the protective agent was: 130g / L skim milk was sterilized at 105°C for 10min; 20g / L of sucrose solution was mixed with 20g / L trehalose solution was autoclaved at 115°C for 20 minutes; the three solutions were mixed at a volume ratio of 1:1:1 to be the protective agent); the LPS modeling group was intraperitoneally injected with 0.2 mL of LPS solution (LPS dissolved in LPS solution with a final concentration of 0.15 mg / kg was made from normal saline) and 0.2 mL of protective agent was administered i...

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Abstract

The invention discloses a lactobacillus mucoase for relieving endotoxin infection and application, and belongs to the technical field of microorganisms. The lactobacillus mucoase CCFM1142 capable of relieving endotoxin infection is obtained by screening through a large number of experiments, and the strain can reduce the content of proinflammatory factors in host blood infected by endotoxin, increase the concentration of anti-inflammatory factors and stabilize intestinal flora composition. By further investigating the application of the strain in food and drug combinations, experiments show that the food or drug containing the lactobacillus mucoase CCFM1142 can be used for preparing foods or drugs for preventing and / or treating endotoxin infection, and has a wide application prospect.

Description

technical field [0001] The invention relates to a strain of mucosal lactobacillus for alleviating endotoxin infection and its application, belonging to the technical field of microbes. Background technique [0002] Endotoxin, also known as lipopolysaccharide, is an important component of the cell wall of Gram-negative bacteria. Endotoxin has a stable structure and is mainly composed of three parts: polysaccharide antigen, core polysaccharide and lipid A (lipid A). Lipid A is a lipidated glucosamine disaccharide that is pyrogenic and the major toxic component of Gram-negative bacterial endotoxins. Under normal circumstances, endotoxins are only released when the bacteria die and dissolve or the bacterial cells are destroyed by artificial methods, and the structure of endotoxins is stable. biological activity. [0003] The intestinal lumen of a normal body contains a large number of intestinal bacteria, and the intestinal mucosa can selectively absorb nutrients and prevent ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P39/02A61P31/04A61P1/12A23C9/123A23L11/65A23L11/50A23L2/38A23L2/84C12R1/225
CPCC12N1/20A61K35/747A61P39/02A61P31/04A61P1/12A23C9/1234A23C11/106A23L2/382A23L2/84A23V2002/00A23V2400/11A23V2200/3204
Inventor 杨波陈卫贾彦陆文伟王刚崔树茂赵建新张灏
Owner JIANGNAN UNIV
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