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Urine helicobacter pylori antibody detection kit and preparation method thereof

A technology for Helicobacter pylori and antibody detection, which is applied in biological testing, measuring devices, immunoassays, etc., can solve problems such as low user acceptance, unsuitable home testing, and inability to detect past infections, and achieves convenient sampling and improved sensitivity. , the effect of high detection sensitivity

Active Publication Date: 2021-04-09
SHANG OUTDO BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] 1. The patent application detects Helicobacter pylori antigen, which can only detect current infection, but cannot detect past infection, which has certain limitations
[0013] 2. The substance detected in this patent application is a fecal sample, and the sampling of the fecal sample is less acceptable to users, and the fecal sample needs to be pretreated during the test
[0014] 3. This patent application requires the use of special medical equipment, so it can only be used in professional medical institutions, and it is not suitable for large-scale censuses, nor is it suitable for home testing, and there are certain limitations
Domestic product development for urine detection of Helicobacter pylori is still in a blank stage

Method used

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  • Urine helicobacter pylori antibody detection kit and preparation method thereof
  • Urine helicobacter pylori antibody detection kit and preparation method thereof
  • Urine helicobacter pylori antibody detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061]Example 1 Preparation and use of the kit of the present invention

[0062]a. Time-resolution fluorescent microsphere mark: Take 100 ul time to resolve fluorescent microspheres, dilute to 1 ml with ultrapure water. Take 40 ul of 50 mg / ml NHS (N-hydroxy succinimide) solution to mix well in the microsphere suspension, then add 40 uL of 50 mg / ml EDC (1- (3-dimethylaminopropyl) -3- Ethyl carbon diimide hydrochloride solution is mixed in a microsphere suspension, and the reaction is 0.5 hours at room temperature. The reaction after the reaction was resuspended with ultrasonic ultrasound, and then the microsphere suspension was centrifuged, sucks the supernatant, and 1 ml of ultra-pure water was added, and then uniformly dispersed evenly, and the activation of the fluorescent microspheres was completed. Take 1 ml of activated microsphere suspension, ultrasonic dispersion is uniform, then dripped with antigens antibody while stirring, the marking amount is 0.01 mg-0.05 mg / ml, prefe...

Embodiment 2

[0085]Example 2: Sensitivity, Specific Verification Comparative Experiment:

[0086]Sample: Take the blood and urine samples of one person, collect 50 samples, including 29 cases of positive cases of Helicobacter, and 22 cases of negative cases. The blood sample was synchronized with the urine sample with the urine sample with the following different detection methods.

[0087]Control group 1: Double antigen sandwich gum gold method: Gold bonding pad contains Helicobacteri antigen A-labeled colloidal gold, nitrocellulose film is packed by Helicobacterium antigen B

[0088]Control group 2: Double antigen sandwich method and indirect method combined with colloidal gold method: Gold bonding pad contains Helicobacter antigen A-labeled colloidal gold and anti-human IgG labeled colloidal gold mixture, nitrocellulose film is packed by H. pylori antigen B

[0089]Control Group 3: Double antigen sandwich method and indirect method combined with fluorescent microspheres: fluorescent pad containing a prac...

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Abstract

The invention discloses a urine helicobacter pylori antibody detection kit and a preparation method thereof. The kit comprises a reagent strip positioned on a bottom plate, wherein the reagent strip sequentially comprises a sample pad, a fluorescent microsphere combination pad, a nitrocellulose membrane and a water absorption pad from a sample adding end; the sample pad is coated with a biotinylated helicobacter pylori antigen B; the fluorescent microsphere combination pad is coated with time-resolved fluorescent microsphere labeled anti-human IgG and time-resolved fluorescent microsphere labeled helicobacter pylori antigen A; a detection line coated with avidin is arranged at one end, close to the fluorescent microsphere combination pad, of the nitrocellulose membrane; and a quality control line coated with goat anti-mouse IgG is arranged at one end, close to the water absorption pad, of the nitrocellulose membrane. Triple amplification of a detection signal is completed, and the sensitivity of the reagent is improved. According to the invention, the helicobacter pylori antibody is detected from urine, and the kit has the advantages of convenience in sampling, complete noninvasiveness and high detection sensitivity.

Description

Technical field[0001]The present invention belongs to the technical field of biodetectation, and in particular, there is a urine H. pylori antibody detection kit and a preparation method thereof.Background technique[0002]The diagnostic method of Helicobacter pylori infection is divided into two major categories of invasive and non-invasive, and invasive examination is based on endoscopic and gastric mucosal biopsy. The gastroscope is inspected under gastroscope, and the examination method has fast urea enzyme experiment. , Direct painting, histological examination, bacterial culture and molecular biology detection (Helicobacter bacteria division and gene mutation detection); non-invasive Helicobacter pylori detection method has urea exhalation experiment, feces Helicobacter antigen detection and Helicobacter Spiral serum antibody test.[0003]HP (Helicobacter pylori) infection is a chronic active gastritis, peptic ulcer, gastric mucosa-related lymphatic tissue lymphoma, and is the mai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/68G01N33/558G01N33/543G01N33/533
CPCG01N33/533G01N33/54313G01N33/558G01N33/56911G01N33/6854G01N2333/195G01N2469/20
Inventor 郜恒骏金杰张小燕李晶
Owner SHANG OUTDO BIOTECH CO LTD
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