Bacillus tequilensis and application thereof

A technology of bacillus and germs, applied in the direction of application, bacteria, fungicides, etc., can solve the problems that the development and utilization of Bacillus tequila is in its infancy, and achieve good development and application prospects, obvious growth-promoting effect, and promote plant growth. Effect

Active Publication Date: 2021-04-13
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the development and utilization of Bacillus tequila is still in its infancy

Method used

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  • Bacillus tequilensis and application thereof
  • Bacillus tequilensis and application thereof
  • Bacillus tequilensis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Isolation, purification and molecular biology identification of bacterial strain M408F10-3F8

[0025] Weigh 10g of wheat rhizosphere soil and add 200mL 0.01M sterile PBS buffer solution, shake at room temperature and 180r / min for 30min to obtain a rhizosphere soil suspension, and dilute to 10% with sterile water -1 、10 -2 and 10 -3 , spread evenly on M408 medium with a coating stick: yeast extract 1g, mannitol 10g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.2g, NaCl 0.1g, agar 15g, distilled water 1000mL, PH=7.2-7.4; after constant temperature cultivation at 30°C for 2 days, pick a single colony for purification.

[0026] DNA extraction was performed in accordance with the procedures of the Column Bacterial DNA Extraction Kit of Shanghai Sangon Bioengineering (Shanghai) Co., Ltd. A pair of bacterial universal primers was selected: F27 5'-AGA GTT TGA TCC TGG CTC AGG-3', P15415'-AAG GAG GTG GTG ATC CAG CCG CA-3'. The reaction conditions were: denaturation at 94°C ...

Embodiment 2

[0054] Example 2 Determination of Bacteriostasis of Bacterial Strain M408F10-3F8

[0055] Plate confrontation method was used to control rape sclerotia (Sclerotinia sclerotiorum), rice sheath blight (Rhizoctonia solani), wheat gibberella (Fusarium graminearum), apple ring pattern (Botryospuaeria berengeriana), tobacco red star (Alteraria alternata), pepper phytophthora (Phytophthora capsicici) ), watermelon blight (Fusarium oxysporum f.sp.niveum), cucumber blight (Fusarium oxysporum.sp.cucumebrium Owen), strawberry blight (Fusarium oxysporum f.sp.fragariae), tomato gray mold (Botrytis cinerea), apple rot (Valsa mali Miyabe et Yamada) and wheat total eclipse (Gaeu-mannomyces graminsis (sacc.) Arx & Olivier Var tritici J.walker) a total of 12 plant pathogenic fungi were tested. First, take the center of the petri dish as the center point, draw a cross on the bottom of the petri dish, and inoculate the strains that have been activated on LB medium at a distance of 2.5 cm from the...

Embodiment 3

[0060] Example 3 Determination of growth-promoting indicators of bacterial strain M408F10-3F8

[0061] First, the strains stored at -80°C were streaked on LB medium, and cultured in a 28°C incubator for 24h to activate. Then inoculate a bacterial needle into a 250mL Erlenmeyer flask filled with 100mL LB liquid medium, and add 1mL L-tryptophan to the Erlenmeyer flask to make the final concentration 100μg / mL. Cultivate at 28°C and 180r / min for 5 days, and carry out 2 biological replicates for each strain. Take 8mL strain fermentation broth, centrifuge at 8000r / min, 4°C, absorb supernatant for quantitative determination of strain IAA content. For the quantitative determination of IAA content, 60 mL of supernatant was drawn into a 2 mL centrifuge tube, and then 120 mL of Salkowski reagent was added for color development under dark conditions, and the absorbance of each treatment at 530 nm was measured with a UV spectrophotometer. At the same time, a standard curve of IAA content...

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Abstract

The invention discloses Bacillus tequilensis and application thereof. The strain is preserved in the China General Microbiological Culture Collection Center on December 7, 2020, the preservation number is CGMCC No. 21317, and the preservation name is Bacillus tequilensis M408F10-3F8. The strain is applied to the field of agricultural biological control, a biological membrane can be formed to be stably colonized at the plant rhizosphere, a high and broad-spectrum inhibition effect can be generated on 12 plant pathogenic fungi and oomycetes, and IAA can be generated to promote plant growth. The control effects of the Bacillus tequilensis bacterial suspension and fermentation liquor on cucumber fusarium wilt potted plants reach 50.00% and 58.00% respectively, the control effect is equivalent to that of a medicament control carbendazim, an obvious growth promoting effect is achieved on cucumbers, and the Bacillus tequilensis is a biocontrol strain with excellent performance and has good development and application prospects.

Description

technical field [0001] The invention belongs to the technical field of agricultural biological control, and in particular relates to a strain of Bacillus tequilensis and an application thereof. Background technique [0002] Plant diseases pose a serious threat to world food production and security. For a long time, the use of chemical pesticides and fertilizers has aggravated problems such as environmental pollution, bacterial strain resistance, and soil micro-ecological imbalance. There is an urgent need to discover microbial products that can replace chemical pesticides and fertilizers. Despite the existence of a large number of microbial products, due to their low colonization ability and short duration in the field, mining broad-spectrum and high-efficiency microorganisms from plant-related microbial communities such as rhizosphere and root endophytes has become an important topic at home and abroad Scholars' research hotspots. [0003] Bacillus is a kind of Gram-posi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01N43/38A01P3/00A01P21/00C05F11/08C05G3/00C05G3/60C12R1/07
CPCC12N1/20A01N63/22A01N43/38C05F11/08C05G3/00C05G3/60
Inventor 王永红他永全闫志强杨龙港何军祝传书冯俊涛马志卿
Owner NORTHWEST A & F UNIV
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