Avocado oil-in-water liquid fermented product as well as preparation method and application thereof
A technology of liquid fermentation and avocado, which is applied in the field of fermentation, can solve the problems of inability to use cosmetic raw materials, incompatibility of avocado oil, difficulty in stable existence of avocado oil, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0050] High-throughput breeding of yeast with high protease production in embodiment 1
[0051] During the fermentation process of liquor, a high protease-producing Yeast Fumembrane was screened, and the process was as follows.
[0052] The formula of solid medium for strain screening is: 20.0g peptone, 1.0g yeast powder, 10.0g skimmed milk, 5.0g glucose, KH 2 PO 4 1.0 g, NaH 2 PO 4 2.4 g, 20.0 g agar, pH 6.8, supplemented with 1000 mL deionized water. After the medium components are prepared, sterilize at 121°C for 20 minutes.
[0053] 1. Primary screening of strains
[0054] Take 0.1g of liquor fermented grains and dissolve it in 1mL of sterile saline, add glass beads and shake evenly. After diluting 10 times, take 200 μL of the mixture and spread it on the solid medium for strain selection, and culture it statically at 30°C for 2 days.
[0055] 2. Re-screening of strains
[0056] Pick the strains showing the transparent circle, and after separation and purification,...
Embodiment 2
[0068] Embodiment 2 produces the screening of biosurfactant bacterial strain
[0069] Liquor fermentation is a mixed-bacteria fermentation process, in which abundant microorganisms adapt to each other and play a synergistic role during the long-term domestication process. The microorganisms screened in the same environment can perform better synergy. Therefore, the screening for surfactant-producing strains was carried out from the growth environment of the yeast of Example 1.
[0070] The currently discovered biosurfactants include many different types, such as glycolipids, lipopeptides, polysaccharide-lipid complexes, phospholipids, fatty acids, and neutral lipids. Different surfactants contain different components, and not all surfactants are suitable for the inclusion of avocado oil. Therefore carry out the screening of the bacterial strain that produces surfactant with the substratum that adds avocado oil, process is as follows.
[0071] Avocado oil liquid medium: 8.0g...
Embodiment 3
[0089] Embodiment 3 Preparation of avocado oil-in-water liquid fermentation product
[0090] 1. Preparation of yeast seed solution
[0091] Under aseptic conditions, a single colony of Saccharomycopsis fibuligera Z1-9 was picked and inoculated into YPD liquid medium. 30℃, 220r·min -1 Shake culture for 24 hours, when the bacterial volume reaches 10 8 CFU mL -1 , the seed solution was obtained.
[0092] 2. Preparation of Lactobacillus pentosus seed solution
[0093] Under aseptic conditions, a single colony of Lactobacillus pentosus (Lactobacillus pentosus) C-7 was picked and inoculated into MRS liquid medium. 37℃, 200r·min -1 Shake culture for 24 hours, when the bacterial volume reaches 10 8 CFU mL -1 , the seed solution was obtained.
[0094] 3. Prepare materials
[0095] Wash fresh avocados with tap water, then rinse and drain with deionized water, and remove the stems; add the above-mentioned avocados to a wall breaker, add 3 times the mass ratio of deionized water...
PUM
| Property | Measurement | Unit |
|---|---|---|
| mobile phase | aaaaa | aaaaa |
| mobile phase | aaaaa | aaaaa |
| quality score | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More