Preparation method of phytosterol nano-liposome
A nano-liposome and phytosterol technology, which is applied in the field of food processing, can solve the problems of poor liposome stability and achieve high embedding efficiency
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[0033] refer to Figure 1-3 , the embodiment of the present invention provides a kind of preparation method of phytosterol nano liposome, comprises the following steps:
[0034] (1) After the lipidoid substance is heated and melted, it is added in an organic solvent dissolved with phytosterol, placed in a vacuum rotary evaporator to remove the organic solvent by rotary evaporation, and forms a uniform film on the wall of the device to obtain the lipidoid substance Lipid film;
[0035] (2) Add phosphate buffer containing emulsifier and polyvinyl alcohol to the lipid film, the lipid film comes off completely, and mixes and disperses evenly to obtain a suspension; the gained suspension is incubated in a water bath and ultrasonically treated successively , to obtain phytosterol-loaded nanoliposomes;
[0036] (3) The phytosterol-loaded nanoliposomes are pressed into the sodium alginate solution through a microporous membrane under the pressure drive by a membrane emulsification d...
Embodiment 1
[0057] Weigh 1 g of glyceryl monostearate and 5 g of medium-carbon chain triglycerides in an eggplant-shaped bottle, heat in a water bath at 40 ° C, and after the solid phase and liquid phase lipids are melted, add 40 ml of ether containing 5 mg of ergosterol dropwise under stirring- Ethanol (8:1, v / v) mixed solution, rotary evaporation in a constant temperature water bath at 35°C until the organic solvent is completely removed, until a uniform lipid film is formed on the eggplant-shaped bottle wall. Then, 300 ml of phosphate buffer solution containing 0.6 g of Tween 80 and 12 g of polyvinyl alcohol was added to the obtained lipid film, immersed in a constant temperature water bath at 70° C. for 10 seconds, vortexed and eluted, and repeated 3 times to obtain a suspension. The resulting suspension was incubated in a water bath at 10° C. for 1 h, and then sonicated for 3 min in an ice-water bath to obtain ergosterol-loaded nanoliposomes. Gained ergosterol-loaded nanoliposomes ar...
Embodiment 2
[0059] Weigh 2g of glyceryl monostearate and 5g of medium-carbon chain triglycerides into an eggplant-shaped bottle, heat in a water bath at 40°C, and after the solid-phase and liquid-phase lipids are melted, add 2.5mg of β-sitosterol and 5mg of In 40ml of dichloromethane solution of stigmasterol, rotary evaporate in 35 ℃ constant temperature water bath until the organic solvent is completely removed, until a uniform lipid film is formed on the eggplant-shaped bottle wall. Then, 300ml of phosphate buffer solution containing 1g of poloxamer P188 and 20g of polyvinyl alcohol was added to the obtained lipid film, immersed in a 70°C constant temperature water bath for 10s, vortexed and eluted, and repeated 3 times to obtain a suspension. The resulting suspension was incubated in a water bath at 10° C. for 1 h, and then sonicated for 3 min in an ice-water bath to obtain nanoliposomes loaded with β-sitosterol-stigmasterol. The resulting nano-liposomes loaded with β-sitosterol-stigma...
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