Normal temperature aerobic treatment method of perishable garbage
A technology based on garbage and aerobic bacteria, applied in the direction of microbial-based methods, chemical instruments and methods, biological water/sewage treatment, etc., can solve problems such as difficult stable operation, large investment in biogas power generation and treatment projects, and improve impact load resistance ability, enhance the stability and recyclability of bacteria agents, and increase the effect of biodegradation efficiency
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[0037] In a specific implementation scenario, the preparation method of the immobilized microbial inoculum includes the following steps:
[0038] 1) Pre-cultivation: take aerobic microbial strains, activate them, and culture them for 24-80 hours at a temperature of 20-45°C and a shaker rotation speed of 50-200 r / min to obtain aerobic microbial bacteria liquid, and then The bacterial liquid is mixed and reconstituted to obtain a concentration of 1 × 10 6 -1×10 91 / mL bacterial suspension for use;
[0039] 2) Carrier preparation: pulverize the plant straw into powder that passes through a 200-400 mesh sieve, mix the fly ash and plant straw powder in a weight ratio of 1:0.05-0.25 to form a mixture, and then mix the glucomannan at 50-60 At ℃, the water bath is dissolved to form a homogeneous solution with a concentration of 40-60g / L, then the mixture is added to the glucomannan solution at a ratio of 2.5-5:2, stirring for 30-60min, and then pressed into an embryo body, Dry at 15...
Embodiment 1
[0050] The preparation method of immobilized microbial inoculum comprises the following steps:
[0051] 1) Pre-cultivation: take aerobic microorganisms Bacillus subtilis, Halobacterium, Bacillus amyloliquefaciens, Pseudomonas brucei, and Pseudomonas virilans. After culturing for 72 hours under the condition of 100r / min, aerobic microbial bacterial liquid was obtained, and then the bacterial liquid was mixed and compounded at a concentration ratio of 1:1:1:1:1, that is, the concentration was 1×10 9 1 / mL bacterial suspension for use;
[0052] 2) Carrier preparation: pulverize the plant straw into powder that has passed a 300-mesh sieve, mix the fly ash and plant straw powder in a weight ratio of 1:0.2 to form a mixture, and then dissolve the glucomannan in a water bath at 60°C to form a concentration It is a homogeneous solution of 45g / L, then the mixture is added to the glucomannan solution at a ratio of material-liquid ratio of 4.5:2. After stirring for 45min, it is pressed i...
Embodiment 2
[0056] The preparation method of the immobilized microbial inoculum, when working, differs from Example 1 only in that step 2) carrier preparation, glucomannan solution is also added with tert-butyl 2-glycolic acid and N-(2 - nitrobenzoyl)glycine, the weight ratio of its addition to the glucomannan solution is 0.75:8 and 0.35:8, respectively.
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