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A high-resistance arsenic-thiocyanide-degrading strain and its application

A technology of thiocyanide and cyanide, which is applied in the direction of bacteria, microorganisms, water pollutants, etc., can solve the problems of hindering the utilization of iodine element, human poisoning, etc., and achieve good degradation effect, low cost and environmental friendliness

Active Publication Date: 2022-07-12
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term exposure to thiocyanate will hinder the body’s utilization of iodine and inhibit thyroid activity. 500mg NaSCN / kg body weight is the minimum lethal dose for animals. If excessive intake of thiocyanate will cause human poisoning, when the blood SCN - It is quite dangerous when the content reaches 150mg / L

Method used

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  • A high-resistance arsenic-thiocyanide-degrading strain and its application
  • A high-resistance arsenic-thiocyanide-degrading strain and its application
  • A high-resistance arsenic-thiocyanide-degrading strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Isolation and identification of Pseudomonas putida strains

[0036] (1) separation and purification

[0037] The inventor isolated a number of strains that can tolerate arsenic and thiocyanide from the domestic sewage of Yanghu wetland in Changsha City, Hunan Province. The specific operation process is as follows:

[0038] P1. Take typical eutrophic activated sludge from Yanghu Wetland Domestic Sewage Pool in Changsha City, Hunan Province;

[0039] P2, configure the LB liquid enrichment medium, add the sludge to the LB medium at a mass ratio of 10%, and enrich and culture it in a constant temperature shaker at 150 r / min for 3 days to obtain an enriched bacterial liquid;

[0040] P3. Continue to inoculate the above enriched bacterial liquid in fresh LB medium at a mass ratio of 10%, and repeat the P2 operation;

[0041] P4, inoculate 10% of the bacterial liquid cultured in P3 in the LB medium containing 200mg / L arsenic and 300mg / L thiocyanate, and cultivate ...

Embodiment 2

[0056] The preparation of embodiment 2 microbial inoculum

[0057] (1) Activation of strains: The slanted strains of the test tube stored on the nutrient agar medium at 4°C were moved to 25°C for activation at room temperature for 4 hours;

[0058] (2) Liquid seed preparation: On the sterile operating table, use 10 mL of sterilized distilled water to pass through the activated test tube slanted bacteria to make a bacterial suspension, and rinse it into a conical flask containing a sterilized liquid seed medium. , 1 test tube is inoculated into 1 conical flask, the pH value is natural, and liquid seeds are obtained;

[0059] (3) Fermentation process: take the liquid seeds made in step (2) and inoculate them in a sterilized fermentation medium with an inoculum of 5% (v / v), and the liquid fermentation preparation can obtain bacteria containing Pseudomonas strains agent.

[0060] Among them, the determination of the conditions of the strain Pseudomonas producing bacteria is as f...

Embodiment 3

[0068] Embodiment 3 measures the influence of different concentrations of thiocyanate on degradation efficiency

[0069] Prepare ionic medium containing 345mg / L, 642mg / L, 836mg / L, 1030mg / L, 1240mg / L, 1468mg / L thiocyanate respectively. Except for thiocyanide, the specific formula of other components is: each liter contains KH 2 PO 4 1.0g, K 2 HPO 4 1.0g, NaCl 0.1g, MgSO 4 ·7H 2 O 0.1g, FeSO 4 ·7H 2 O 0.04g, anhydrous CaCl 2 0.005g, glucose / SCN - =15(w / w), 2mL of trace elements, deionized water to 1L, pH 7.7, cultured in a constant temperature shaker at 30°C; the formula of trace elements is: CuCl 2 40mg, KI 100mg, MnCl 2 ·H 2 O 400mg, ZnCl 2 ·7H 2 O 400mg, HBO 3 500mg, NaMoO 4 ·2H 2 O 195 mg, make up to 200 mL with deionized water.

[0070] The microbial inoculum addition amount obtained in the embodiment 2 is 2% (w / w), the residual thiocyanate concentration in the solution is measured by sampling at a certain time, and the selected assay method is the fer...

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Abstract

The invention discloses a highly arsenic-resistant thiocyanate-degrading bacterial strain and its application. The bacterial strain is identified as Pseudomonas putida, numbered TDB-1, and is preserved in the General Committee of the China Microorganism Culture Collection and Management Committee. Microbiology Center, the deposit number is CGMCC NO.21602. The strain has the characteristics of high arsenic resistance, and the inoculum prepared by the strain can effectively degrade thiocyanide in a high-concentration arsenic environment, and the degradation speed is fast and the effect is good.

Description

technical field [0001] The invention relates to the field of microorganisms and thiocyanate degradation, and more particularly, to a high arsenic thiocyanate degradation bacterial strain and application thereof. Background technique [0002] Thiocyanide is a compound containing S-C≡N, and its toxicity is about 1 / 7 of that of cyanide. In the cyanide heap leaching site of gold mine, the conversion of cyanide is derived from the conversion of cyanide, and the conversion rate can be as high as 50% or more in the reducing environment such as sulfide ore. Long-term exposure to thiocyanate will hinder the body's utilization of iodine and inhibit thyroid activity. 500mg NaSCN / kg body weight is the minimum lethal dose for animals. Excessive intake of thiocyanate will cause human poisoning. SCN - It is quite dangerous when the content reaches 150mg / L. In addition, thiocyanate can also be converted into cyanide under certain conditions. In the acidic solution of thiocyanate, zinc c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F101/18C12R1/40
CPCC12N1/20C02F3/34C02F2101/18Y02P10/20
Inventor 廖骐贺立旭唐崇俭唐溪张钦雅曹维杨志辉杨卫春李青竹王海鹰
Owner CENT SOUTH UNIV
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