Anti-KPC type carbapenemase hybridoma cell strain, monoclonal antibody and application

A hybridoma cell line and carbapenemase technology, which can be used in applications, anti-enzyme immunoglobulins, instruments, etc., can solve the problems of limited range of clinical treatment drugs, clinical infection control and treatment difficulties, etc. Clinical efficacy of infection control and treatment
CN112980804AActive Publication Date: 2021-06-18TIANJIN ERA BIOLOGY TECH CO LTD +3
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TIANJIN ERA BIOLOGY TECH CO LTD
Publication Date
2021-06-18

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Abstract

The invention provides an anti-KPC type carbapenemase hybridoma cell strain, a monoclonal antibody and application. The hybridoma cell strain capable of stably secreting the anti-KPC type carbapenemase antibody and a variable region sequence of the hybridoma cell strain are obtained by screening a mouse hybridoma monoclonal antibody and cloning an Ig variable region gene by an RT-PCR method. Through systematic evaluation, the anti-KPC type carbapenemase monoclonal antibody has better performance in all aspects, so that the anti-KPC type carbapenemase monoclonal antibody is suitable for being used as an immunodiagnostic reagent for in vitro diagnosis of KPC type carbapenemase, and the titer reaches 1: 1280000 or above; and a prepared kit or microfluidic chip can be used for early typing of drug-resistant strains, guiding medication and assisting clinical infection control and treatment.
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Description

Technical field

[0001] The present invention belongs to the technical field of antibody preparation, and more particularly to an anti-KPC type carbon oxylidease hybrid tumor cell line, monoclonal antibody and application. Background technique

[0002] Carbapenem antibiotic infection control clinical isolates one of the most effective drugs, biological production carbapenemases (CPO) and carbapenem resistance Enterobacter its broad spectrum resistance has become a global public Health issues, patient treatment is very limited. Enterus bacteria are important pathogens in hospital infection and community acquired infections. In recent years, due to the extensive use of cephalosporins, Enterobacteriaceae producing extended-spectrum β- lactamase (extended spectmbeta-lactamases, ESBLs) Strain Amp C and proportion of the enzyme was the drug of choice clear upward trend in the treatment of infections such strains It is a carbon Popular bilochemistry. However, along with imipenem and mero...

Claims

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