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Preparation and application method of an injectable composite chitosan hydrogel for bone repair

A technology of chitosan and bone repair, which is applied in pharmaceutical formulations, prostheses, drug delivery, etc., can solve the problems of unsatisfactory cell activity, easy infection or immune rejection, and low migration rate, so as to achieve rapid response and promote Excellent adhesion and biocompatibility

Active Publication Date: 2022-05-31
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recently, autologous transplantation and allogeneic transplantation are important treatments to promote bone defect healing and bone tissue remodeling, but both have certain disadvantages, such as unsatisfactory cell viability and low migration rate of autologous transplantation, and allogeneic transplantation is easy to cause infection or immune rejection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1) Weigh 1.5g of chitosan powder with a viscosity average molecular weight of 100W and a degree of deacetylation of 90%, 1.75g ​​of N-isopropylacrylamide, 0.175g of ammonium persulfate, dispersed in 95mL of deionized water, and then added 0.5 After stirring and dissolving evenly, the solution was heated to 60 °C for 8 hours, and then the solution was transferred into a dialysis bag, repeatedly dialyzed with deionized water, and freeze-dried to obtain a poly-N-isopropylacrylamide graft-modified shell. Glycan powder;

[0030] 2) Add 1-20 parts of poly-N-isopropylacrylamide graft-modified chitosan powder in step 1) into 80-99 parts of PBS solution, stir and dissolve evenly at 10° C. to obtain a hydrogel precursor solution;

[0031] 3) Sterilize the hydrogel precursor solution in step 2), mix 30 parts of the hydrogel precursor solution in 2), 5 parts of the bone marrow mesenchymal stem cell suspension and 0.1 part of HAP at 10°C blending and mixing to obtain an injectable...

Embodiment 2

[0034] 1) Weigh 1.5g of chitosan powder with a viscosity average molecular weight of 100W and a degree of deacetylation of 90%, 1.75g ​​of N-isopropylacrylamide, 0.175g of ammonium persulfate, dispersed in 95mL of deionized water, and then added 0.5 After stirring and dissolving evenly, the solution was heated to 60 °C for 8 hours, and then the solution was transferred into a dialysis bag, repeatedly dialyzed with deionized water, and freeze-dried to obtain a poly-N-isopropylacrylamide graft-modified shell. Glycan powder;

[0035] 2) Add 1-20 parts of poly-N-isopropylacrylamide graft-modified chitosan powder in step 1) into 80-99 parts of PBS solution, stir and dissolve evenly at 10° C. to obtain a hydrogel precursor solution;

[0036] 3) Sterilize the hydrogel precursor solution in step 2), mix 20 parts of the hydrogel precursor solution in 2), 5 parts of the bone marrow mesenchymal stem cell suspension and 0.2 parts of polypeptide molecules at 10°C. Blending in an environm...

Embodiment 3

[0039] 1) Weigh 1.5g of chitosan powder with a viscosity average molecular weight of 100W and a degree of deacetylation of 90%, 1.75g ​​of N-isopropylacrylamide, 0.175g of ammonium persulfate, dispersed in 95mL of deionized water, and then added 0.5 After stirring and dissolving evenly, the solution was heated to 60 °C for 8 hours, and then the solution was transferred into a dialysis bag, repeatedly dialyzed with deionized water, and freeze-dried to obtain a poly-N-isopropylacrylamide graft-modified shell. Glycan powder;

[0040] 2) Add 1-20 parts of poly-N-isopropylacrylamide graft-modified chitosan powder in step 1) into 80-99 parts of PBS solution, stir and dissolve evenly at 10° C. to obtain a hydrogel precursor solution;

[0041] 3) Sterilize the hydrogel precursor solution in step 2), and sterilize 50 parts of the hydrogel precursor solution in 2), 5 parts of the osteoblast cell suspension and 1 part of HAP at 10°C. Blend and mix evenly to obtain an injectable composi...

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PUM

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Abstract

The invention relates to a method for preparing and using an injectable composite chitosan hydrogel for bone repair. The hydrogel is mainly composed of modified chitosan, inner cells and cell growth-promoting factors. Firstly, the hydrogel precursor solution is prepared by emulsion polymerization, the main component is chitosan macromolecules grafted with poly-N-isopropylacrylamide, the hydrogel can be in solution state at room temperature, and is stable in human physiology. Rapid gelation at ambient temperature. By mixing with cell suspension and cell growth-promoting factors, the resulting composite solution can realize in-situ rapid gelation at the site of bone tissue injury, and achieve in-situ fixation of cells, which can not only provide good support for the injury site, but also promote Adhesion, proliferation and differentiation of internal cells. Due to the simple preparation, convenient use, rapid response, and good biocompatibility, the hydrogel can be used in many biomedical related fields such as tissue repair, drug controlled release, cell culture, and cancer treatment.

Description

technical field [0001] The invention relates to a chitosan-based injectable hydrogel, in particular to a preparation and use method of an injectable composite chitosan hydrogel for bone repair. Background technique [0002] How to efficiently repair bone tissue damage or defect caused by trauma, infection, tumor and other factors is still an important challenge in clinical medicine. Recently, autologous transplantation and allogeneic transplantation are important treatment methods to promote bone defect healing and bone tissue remodeling, but both have certain drawbacks, such as unsatisfactory cell viability and low migration rate of autologous transplantation, and allogeneic transplantation is prone to infection. or immune rejection. [0003] An ideal bone tissue repair and regeneration material should not only provide short-term support at the bone defect site with appropriate mechanical strength, but also promote cell adhesion, proliferation, differentiation, and mineral...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F251/00C08F220/54A61L27/20A61L27/38A61L27/52A61L27/54A61L27/58
CPCC08F251/00A61L27/20A61L27/3834A61L27/3821A61L27/3817A61L27/3847A61L27/3852A61L27/54A61L27/52A61L27/58A61L2430/06A61L2430/02A61L2400/06A61L2300/412A61L2300/414C08F220/54C08L51/02
Inventor 胡巧玲姜质琦董晓飞
Owner ZHEJIANG UNIV
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