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Formula milk for sterile animals and preparation method thereof

A technology for sterile animals and formula milk, which is used in the molding or processing of animal feed, animal feed, animal feed, etc., can solve the problems of low survival rate of sterile mice, acquired malnutrition, etc., to avoid environmental impact and low cost. , easy to formulate effect

Pending Publication Date: 2021-06-29
深圳净拓生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Part of the formula milk is easy to sterilize, but the formula is extremely simple. The formula milk composed of a single nutrient component is used in the process of artificial feeding of germ-free mice, which often leads to a low survival rate of germ-free mice and is prone to acquired malnutrition.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0024] The present invention proposes a preparation method of formula milk for aseptic animals, comprising the following steps:

[0025] S1, adding 10-15 parts by weight of infant milk powder, 4-6 parts by weight of goat milk and 9-11 parts by weight of light cream into purified water to obtain a milk powder solution;

[0026] S2. Prepare 2-3 parts by weight of antibacterial agent with purified water to prepare antibacterial agent solution; dissolve 2-3 parts by weight of chitosan in hydrochloric acid solution, and adjust the pH value to weak acidity; Dissolve gelatin in purified water to prepare gelatin solution, mix chitosan solution and gelatin solution, add 0.2-0.4g emulsifier to obtain chitosan-gelatin mixed emulsion, drop antibacterial agent solution into chitosan-gelatin and mix In the emulsion, stir continuously until the wet capsules are fully formed, filter, rinse the wet capsules, and finally obtain antimicrobial agent microcapsules, and add them to the milk powder ...

Embodiment 1

[0038] Add 13g of infant milk powder, 5g of goat milk and 10g of whipped cream into 60mL of purified water, and obtain a milk powder solution after fully dissolving; heat 10g of cardamom in water to obtain a plant extract, and add 1g of nano-titanium dioxide to the plant extract, 20 Heating and stirring at a temperature of ℃ for 1 h, adding 1 g of tannin, and stirring until flocs are formed, which precipitate after standing, and the filtered matter is dried to obtain an antibacterial agent, and 2 g of antibacterial agent is prepared into an antibacterial agent solution with 20 mL of purified water. Dissolve 2g of chitosan in 20mL of hydrochloric acid solution with a concentration of 0.8mol / L, and adjust the pH value to 6, dissolve 2g of gelatin in 20mL of purified water to prepare a gelatin solution, mix the chitosan solution with the gelatin solution, add 0.2g fatty acid soap (emulsifier), stirred and emulsified for 10-20min to obtain chitosan-gelatin mixed emulsion, drop 10mL...

Embodiment 2

[0040]Add 13g of baby milk powder, 5g of goat milk and 10g of whipped cream into 60mL of purified water, and after fully dissolving, a milk powder solution is obtained; heat 20g of Lonicera indigo in water to obtain a plant extract, and add 2g of nano oxide to the plant extract Aluminum, heat and stir at 40°C for 2 hours, add 2g of lignin, and stir until flocs are formed, precipitate after standing, and dry the filter to obtain an antibacterial agent, prepare 3g of antibacterial agent with 30mL of purified water to prepare an antibacterial agent solution, 3g of chitosan was dissolved in 20mL of hydrochloric acid solution with a concentration of 0.8mol / L, and the pH value was adjusted to 6, 2g of gelatin was dissolved in 20mL of purified water to prepare a gelatin solution, and the chitosan solution and the gelatin solution were Mix, add 0.4g fatty acid soap (emulsifier), stir and emulsify for 20min to obtain chitosan gelatin mixed emulsion, drop 10mL antibacterial agent solutio...

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Abstract

The invention discloses formula milk for sterile animals and a preparation method thereof. The preparation method comprises the following steps that infant milk powder, goat milk and whipping cream are added into purified water to obtain a milk powder solution; an antibacterial agent is prepared into an antibacterial agent solution by using the purified water; chitosan is dissolved in a hydrochloric acid solution, the pH value is adjusted to weak acidity, gelatin is dissolved in the purified water to prepare a gelatin solution, the chitosan solution and the gelatin solution are mixed to obtain a chitosan and gelatin mixed emulsion, the antibacterial agent solution is dropwise added into the chitosan and gelatin mixed emulsion, stirring is continuously carried out until a wet capsule is fully formed to obtain the antibacterial capsule, filtering is carried out, the wet capsule is washed to finally obtain antibacterial agent microcapsules, and the antibacterial agent microcapsules are added into the milk powder solution; sterilizing is carried out after the milk powder solution is fully dissolved, and B-group multivitamin injection is added into the milk powder solution in a sterile environment; and cyclodextrin is dissolved in water to obtain a cyclodextrin solution, and the milk powder solution is added into the cyclodextrin solution to obtain the formula milk.

Description

technical field [0001] The invention relates to the technical field of animal formula milk, in particular to a formula milk for sterile animals and a preparation method thereof. Background technique [0002] Sterile animals refer to animals raised in an isolation system that cannot detect any microorganisms and parasites inside and outside the body after testing. This type of animal is the offspring animal obtained by artificial cesarean section under sterile conditions, and then placed in a sterile environment. It is bred by artificial milk or aseptic "nanny" animal milk substitute in the bacteria isolation system. At present, germ-free animals are mainly used in basic medicine, especially in the research of immunity and intestinal microecology, providing new research directions for disease treatment methods and drug development. At present, sterile animals are mainly rats and mice. The preparation methods include aseptic caesarean section and aseptic embryo transfer. In t...

Claims

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Application Information

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IPC IPC(8): A23K10/20A23K20/158A23K20/163A23K20/147A23K20/174A23K10/30A23K40/30A23K50/50
CPCA23K10/20A23K20/158A23K20/163A23K20/147A23K20/174A23K10/30A23K40/30A23K50/50
Inventor 谭验潘超敏牛海涛尹意铭
Owner 深圳净拓生物科技有限公司
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