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Paenibacillus terrae for preventing and treating gray mold of paris polyphylla and application of paenibacillus terrae

A technology of bacillus and gray mold, applied in the direction of application, bacteria, and fungicides, can solve problems that threaten the quality of heavy buildings, excessive pesticide residues, environmental pollution, etc., achieve good promotion value and ecological benefits, increase income, and be compatible good sex effect

Pending Publication Date: 2021-07-23
湖北省农业科学院中药材研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the lack of registered medicines of traditional Chinese medicinal materials, the phenomenon of unavailable medicines is serious, resulting in frequent incidents of illegal use of pesticides
The abuse of pesticides in actual production and the long-term use of a single agent have led to problems such as excessive pesticide residues, environmental pollution, and drug resistance of pathogenic bacteria, which seriously threaten the quality of Zhonglou.

Method used

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  • Paenibacillus terrae for preventing and treating gray mold of paris polyphylla and application of paenibacillus terrae
  • Paenibacillus terrae for preventing and treating gray mold of paris polyphylla and application of paenibacillus terrae
  • Paenibacillus terrae for preventing and treating gray mold of paris polyphylla and application of paenibacillus terrae

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Experimental program
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Effect test

Embodiment 1

[0037] The isolation, screening and identification of embodiment 1 target bacterial strain

[0038] The target strain was isolated from the leaves of Chonglou in Enshi City, Hubei Province, and healthy leaves were taken, sterilized with 75% alcohol for 1 minute, rinsed with sterile water for 3 times, air-dried under sterile conditions, ground with sterile water, and absorbed the mixture Spread on NA plates after dilution, culture at 28°C for 72 hours, pick single colony for purification and culture. Botrytis cinerea cinerea 3cm, place the isolated endophytic bacteria on the leaves of P. cinerea according to the criss-cross method, and after 3 days, wait for the control to cover the dish, measure the distance from the outer edge of the bacterial colony to the gray mold mycelium, repeat 3 times, and select the distance The largest bacteria were used for later research.

[0039] After testing, the target strain is a Gram-positive bacterium, the bacterium is rod-shaped, can form ...

Embodiment 2

[0042] The preparation of embodiment 2 target bacterial agents

[0043] S1. Take a loop of the target strain with an inoculation loop, spread it on the NA medium, and culture it at 28° C. for 72 hours. The formula of NA solid medium is as follows: Take 5.0g of beef extract, 10.0g of peptone, 5g of NaCl, 15g of agar, 1000mL of purified water, pH 7.2-7.4, and sterilize under high pressure steam at 121℃ for 30min.

[0044] S2. Transfer the target single colony activated in step S1 into a 250ml Erlenmeyer flask with 100ml NB liquid medium, and culture it at 200rmp and 28°C for 72 hours to obtain a fermented seed solution. The formula of NB liquid medium is as follows: take 5.0g of beef extract, 10.0g of peptone, 5g of NaCl, 1000mL of purified water, pH 7.2-7.4, and sterilize at 121°C for 30min by high-pressure steam.

[0045] S3. Inoculate the inoculation amount of the fermented seed liquid in step S2 into the Erlenmeyer flask containing 100ml NB according to the ratio of 1:150 (...

Embodiment 3

[0050] The influence of embodiment 3 target bacterium fermentation supernatant liquid on the Botrytis cinerea

[0051] Take 500 μL of the activated target bacterial solution and inject it into 100 mL NB, and incubate at 28°C and 180 r / min for 72 hours to obtain the fermentation stock solution. The fermentation stock solution was centrifuged at 12,000 r / min for 10 min, the supernatant was collected, and the fermentation filtrate was obtained by filtering with a 0.22 μm microporous membrane. Get filtrate and the PDA mixing of about 45 ℃, make the final concentration of filtrate be 5% and 10%, inoculate Botrytis cinerea mycelia block (diameter 0.5cm) in plate center after cooling, set blank control, each handles three repetitions, Cultured in a 21°C incubator. Measure the diameter of the colony after the control plate is overgrown (4 days), and calculate the inhibition rate. Inhibition rate (%) = (colony diameter of pathogenic bacteria in the control group - colony diameter of ...

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Abstract

The invention belongs to the technical field of plant disease and pest control, and particularly relates to paenibacillus terrae for preventing and treating gray mold of paris polyphylla and an application of the paenibacillus terrae. The paenibacillus terrae strain provided by the invention comes from the interior of a paris polyphylla leaf and has good compatibility with a self-circulation system of the paris polyphylla. The microbial agent is simple to prepare, low in cost, non-toxic, harmless and free of environmental pollution, is beneficial to safe and effective control of gray mold of the paris polyphylla and improvement of the yield and quality of the paris polyphylla, and is also beneficial to green cultivation of the paris polyphylla, protection of a farmland ecosystem and promotion of sustainable development of the paris polyphylla industry. The microbial agent is beneficial to improving the planting soil of the paris polyphylla, increasing the diversity of microorganisms in the soil and promoting the yield of the paris polyphylla and the content of effective components.

Description

technical field [0001] The invention belongs to the technical field of plant disease and insect pest control, and in particular relates to a Paenibacillus aerene for preventing and treating Botrytis cinerea and its application. Background technique [0002] Paris polyphylla is a medicinal plant of Liliaceae, which is widely distributed in Sichuan, Yunnan, Guizhou, Hunan, Hubei and other places in my country. It has the functions of inhibiting bacteria, clearing away heat and detoxifying, reducing swelling and relieving pain. Treatment of venomous snake bites, treatment of convulsions and convulsions, anti-cancer and protection of the cardiovascular system, etc. In addition, Zhonglou is the main raw material of Chinese patent medicines such as Yunnan Baiyao, Gongxuening, and Jidesheng snake medicine. Due to the slow growth of chrysanthemums, coupled with long-term excessive harvesting and digging by people, the resources of wild chrysanthemums are on the verge of exhaustion, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/25A01P3/00C12R1/01
CPCC12N1/20A01N63/25
Inventor 游景茂唐涛王帆帆段媛媛郭杰郭晓亮
Owner 湖北省农业科学院中药材研究所
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