31 results about "Microbial biodiversity" patented technology

The invention discloses a method for restoring soil heavy metal pollution by coupling soybean urease with a charcoal cementing agent, and relates to the technical field of soil heavy metal pollution restoration, and the method comprises the following steps: dynamic microbiome regulation and control: introducing photosensitive protein into soil functional bacteria; the acyl homoserine lactone analogue is synthesized and applied to soil, and is competitively combined with a microbial quorum sensing receptor to block an inhibitor to secrete a signal channel; according to the method, microbial urease secretion is regulated and controlled through optogenetics, an inhibitor signal is blocked through quorum sensing interference, and a microbiome is precisely regulated and controlled; the pH-responsive nanocapsule releases an inhibitor in a targeted manner, and the magnetic-responsive nanochain dynamically captures interfering bacteria, so that the limitation of passive adsorption is broken through; the gene editing plant releases coumarin to inhibit fungal metabolism, and the engineering mycorrhizal fungi physically shields an inhibitor to construct cross-biosphere cooperative defense; the urease activity and the heavy metal removal rate are remarkably improved, meanwhile, the soil microbial diversity is maintained, and the soil specificity interference problem is solved.

The invention discloses a saline-alkali soil improver and a use method thereof, and belongs to the technical field of soil improvement. The modifier comprises the following components in parts by mass: 60-80 parts of an organic carrier, 10-20 parts of an inorganic calcium material, 5-9 parts of a compound microbial agent, 10-20 parts of a sustained-release material, 5-10 parts of an acidity regulator and 15-23 parts of a plant growth promoter. The preparation method comprises the following steps: crushing the raw materials, embedding the sustained-release microspheres, loading the microbial inoculum, and granulating and drying. Through cooperation of multiple mechanisms such as calcium and sodium replacement, pH reduction by organic acid and aggregate formation promotion by microorganisms, differential application strategies are designed for light, medium and severe saline-alkali soil, salt-tolerant crop planting is matched, the yield of wheat, alfalfa and the like is increased by 18%-30%, the soil organic matter is increased to 2.1%, and the microbial diversity index is increased by 133%. The method has the advantages of long-acting salt control, ecological restoration and cost, and is suitable for treatment of coastal and inland saline-alkali soil.

The invention relates to a microbial agent for preventing and treating root rot of radix tinosporae and application of the microbial agent. The microbial agent is prepared from the following components: bacillus velezensis with the preservation number of CCTCC (China Center for Type Culture Collection) No: M2022923, bacillus subtilis with the preservation number of CCTCC No: M20221207, a marigold extract, amino acid, humic acid and medium trace elements. When the microbial agent is applied to the planting process of radix tinosporae, the physicochemical property of radix tinosporae planting soil can be remarkably improved, the enzyme activity and the microbial diversity can be improved, the content of phenolic acid and fusarium toxin in the soil can be reduced, the disease index of radix tinosporae root rot caused by fusarium solani and pythium aphanidermatum can be further reduced, and finally prevention and control of radix tinosporae root rot can be achieved.

The invention relates to the technical field of agricultural cultivation, in particular to a farmland soil ecological structure restoration and soil fertility improvement cultivation method which comprises the steps that 1, the soil state is evaluated; 2, tillage depth is determined; step 3, adding organic materials; 4, tillage operation is carried out, a tillage machine is used for carrying out soil tillage, the tillage depth is the tillage depth determined in the step 2, and the tillage operation adopts a layered tillage mode including shallow tillage and deep tillage; step 5, inoculating a microbial agent, namely inoculating the soil microbial agent based on the soil microbial diversity and the soil enzyme activity in the step 1 after the cultivation operation; 6, covering and maintaining are conducted, after cultivation and inoculation, soil covering and maintaining are conducted, and using of covering materials and precise water and fertilizer management are included. Accurate soil evaluation and personalized tillage depth determination enable soil remediation to be more targeted and effective; organic materials and microorganisms are reasonably matched and used, so that the soil fertility and the biological activity are improved.

The invention relates to the technical field of microbial agents, and discloses a rapid straw decomposition microbial agent, which comprises the following raw materials by weight: 10-30% of a microbial strain, 5-15% of a nutrient substance, 40-70% of a carrier, 2-4% of a stabilizer, 1-2% of a pH regulator, 1-2% of an enzyme preparation, and 1-2% of a plant growth regulator. The microbial agent disclosed by the invention contains nutrient substances, a stabilizer, a pH regulator, an enzyme preparation and a plant growth regulator, and the nutrient substances are beneficial to rapid propagation and efficient metabolism of microbial strains, so that the overall effect of the microbial agent is improved; the stability and activity of the microbial inoculum can be maintained through the stabilizer, the microbial inoculum is prevented from being stored and transported, and the shelf life of the microbial inoculum can be prolonged; through the plant growth regulator, the crops can be more robust and high in yield in the growth process, and the microbial agents can also improve the soil structure, improve the diversity of soil microorganisms and enhance the stress resistance and immunity of the crops, so that the yield and quality of the crops are comprehensively improved.

The invention belongs to the technical field of soil improvement, and particularly relates to a saline-alkali soil conditioner as well as a preparation method and application thereof. The saline-alkali soil conditioner is a compound microbial agent for saline-alkali soil improvement. The compound microbial agent is prepared by jointly mixing and fermenting a seed solution of lactobacillus acidophilus, a seed solution of lactobacillus casei and a seed solution of streptococcus thermophilus; the volume ratio of the seed solution of the lactobacillus acidophilus to the seed solution of the lactobacillus casei to the seed solution of the streptococcus thermophilus is 1: (0.8-1.2): (0.8-1.2). The saline-alkali soil conditioner provided by the invention has the characteristics of quickly reducing alkaline harm of saline-alkali soil, promoting crop growth, improving nutrient absorption and utilization, improving microbial diversity and functional activity of soil, and being easy to apply, low in cost, quick in effect and the like.

The invention relates to the technical field of bacterial fertilizers, in particular to a composite microbial fertilizer and a preparation method thereof. The composite microbial fertilizer is prepared from the following raw materials in parts by mass: 100 to 200 parts of sterilized organic manure, 50 to 150 parts of urea, 10 to 50 parts of ammonium humate, 50 to 70 parts of crop straw, 30 to 50 parts of hydroxyapatite, 80 to 200 parts of composite bamboo charcoal powder, 1 to 10 parts of a composite microbial agent, 10 to 20 parts of chitosan, 1 to 3 parts of polyethylene glycol and 40 to 120 parts of calcium chloride. The soil conditioner disclosed by the invention is beneficial to reducing the accumulation of soil salinity, reducing the salinization degree, enhancing the water and fertilizer retention performance of the soil, creating a good condition for the growth of crop roots, improving the soil structure, increasing the water retention and the air permeability, improving the microbial diversity and the fertility of the soil, and remarkably improving the yield and the quality of crops.

The invention discloses an application of intestinal microbial flora in preparation of a microecological preparation for treating gas explosion brain injury, and the neuroprotective effect of fecal microbial transplantation on GE-induced TBI rats, including neurological dysfunction, neuropathological change and neuroinflammation, is researched by adjusting a microflora-intestinal tract-brain axis. According to the invention, a model of intestinal flora imbalance and brain injury of male rats is established by using a fecal microbial transplantation technology, and then non-targeted metabonomics and microbial diversity analysis are carried out to explore bacteria with potential functions. Meanwhile, the intestinal microbiota composition, the intestinal and brain barrier functions and the number of regulatory T cells of the brain are evaluated. A potential anti-inflammatory mechanism determined by integrating microbiome and metabolome analysis relates to a peripheral immune pathway, and a promising strategy is provided for relieving GE-induced TBI.

The invention relates to the field of tea planting, in particular to a method for interplanting bauhinia trees and tea trees based on ecological niche complementation and application. According to the method for interplanting the bauhinia trees and the tea trees based on ecological niche complementation, the bauhinia trees and the tea trees are interplanted in soil, the row spacing of tea tree planting is 0.5 m * 1 m, and the row spacing of white-flower bauhinia trees is 1 m * 2 m. The method has the following effects: 1, the soil fertility is effectively improved; 2, the diversity of soil microorganisms Alpha and Beta is improved through intercropping; 3, diseases such as tea anthracnose and tea ring spot are effectively reduced;

The invention belongs to the technical field of mining area ecological restoration and soil microbial engineering, and particularly relates to a loess sandstorm coal mine area reclaimed soil microbial diversity maintaining method. A'plant-microorganism-soil 'coordinated regulation and control system is designed, a'strip-type crop rotation' planting mode is provided, and microbial communities with specific functions are synchronously inoculated, so that a microbial network structure of the reclaimed soil is reconstructed, a microbial interaction network is constructed, and the complexity and stability of the reconstructed microbial network are evaluated; and dynamically regulating and controlling the reconstructed soil microorganism system according to an evaluation result to stably maintain the biological diversity of the micro soil. The method provided by the invention is suitable for mining area reclamation scenes with strong drought and wind erosion and heavy metal pollution, and has the advantages of wide adaptability, high microbial community construction efficiency, strong stress resistance and disturbance resistance and the like. And a systematized and refined regulation and control path can be provided for microbial diversity recovery of the reclaimed soil in the loess sandstorm coal mine area.

The utility model relates to the technical field of soil microorganism detection, and discloses a tobacco soil microorganism diversity detection device which comprises a detection box, the top of the detection box is fixedly connected with a treatment box, and the top of an inner cavity of the treatment box is provided with a stirring and scattering assembly. A screening assembly is arranged at the bottom of an inner cavity of the treatment box, and a reciprocating driving assembly is arranged on one side of the top of the detection box. After a power supply is switched on and a motor is started, the motor drives a rotating rod to rotate, so that a main bevel gear and an auxiliary bevel gear are linked, a stirring shaft and stirring blades rotate, sampled soil fed into a treatment box can be effectively scattered and stirred, meanwhile, a convex piece is driven to rotate to intermittently extrude a movable sleeve, and a reciprocating rod and a screening frame are driven to transversely shake; by means of the stirring and shaking combined mode, the soil can be stirred to the proper granularity, and the soil can be efficiently screened after falling into the screening frame.

The invention relates to the field of intelligent monitoring and control systems, in particular to a mining area ecological restoration intelligent monitoring and regulation and control system based on multi-source data fusion, which comprises a data acquisition module, a data processing module, a restoration effect monitoring module and a regulation and control module, the data processing module is used for preprocessing the original data acquired by the data acquisition module, and the restoration effect monitoring module is used for judging the restoration effect of the mining area according to the preprocessed soil related data, vegetation related data and water quality related data. And the regulation and control module is used for regulating and controlling each device for ecological restoration of the mining area according to the restoration effect obtained by the restoration effect monitoring module. According to the scheme, ecological community parameters such as vegetation diversity and soil microbial diversity are introduced into judgment of the remediation efficiency, and the biological ecology basis for remediation efficiency evaluation is improved.

The invention discloses a targeted biological bacterium coupling method for comprehensive treatment of coastal vegetable salt damage, heavy metal and pesticide residues and application, and relates to the field of agricultural biotechnology and soil remediation. According to the method, bacillus subtilis, bacillus mucilaginosus, brevibacillus laterosporus, pseudomonas fluorescens, arbuscular mycorrhizal fungi and host original flora extracted from healthy vegetable rhizosphere soil are subjected to coupled symbiotic culture, and target flora with multiple functions is formed. The functional flora is compounded with a nutrient carrier containing potassium humate and fulvic acid, a seaweed extract and a specific salt-resistant additive to prepare the multifunctional water-soluble fertilizer or fungicide. After the compound fertilizer is applied to a coastal vegetable production base, the synergistic effects of degrading soil pesticide residues, passivating heavy metals, enhancing the salt tolerance of vegetables and improving the soil fertility and microbial diversity can be synchronously realized, the compound obstacle problem of coastal vegetable production is fundamentally solved, and the yield, quality and safety of vegetables are guaranteed.

The invention relates to a soil conditioner for improving facility vegetable field secondary salinization and a preparation method and application thereof, and belongs to the technical field of facility agriculture soil improvement, the soil conditioner for improving facility vegetable field secondary salinization comprises mushroom dregs, cow dung, humic acid, a microbial agent and a microbial protective agent; the microbial agent is prepared from brevibacillus laterosporus, bacillus megaterium and bacillus subtilis; the microbial protective agent comprises the following components: alginate oligosaccharide, vegetable oil, ectoine and fumaric acid. The modifier provided by the invention is beneficial to improving soil organic matters, improving the utilization rate of soil nutrients, preventing soil hardening, improving the diversity of soil microorganisms and reducing the influence of a salt stress environment on vegetable growth.

This invention belongs to the technical field of biodiversity research equipment, specifically relating to a device for assessing microbial diversity indicators using aquatic sediments. It includes a sampling tube and a branch pipe connected to the sampling tube, a positioning plate located at the lower end of the branch pipe, a positioning foot and a sampling end located at the lower end of the positioning plate and perpendicular to the ground. The positioning plate contains a sampling channel connected to the sampling end, which is sequentially connected to the branch pipe and the sampling tube to form a sediment channel. The side wall of the sampling tube is equipped with at least two sets of sampling power mechanisms. Each sampling power mechanism includes a mounting box, an L-shaped air passage located within the mounting box and connected to the inside of the sampling tube, a motor located at the upper end of the mounting box, and a first screw coaxially arranged with the motor's output end. This invention, through the cooperation of the sampling power mechanism and the transmission mechanism, can create negative pressure within the sediment channel by starting the motor to draw samples, while simultaneously driving the positioning foot to move downwards to position the device on the bottom of the water, achieving two goals at once.

The application discloses a heavy metal contaminated soil remediation method based on a southeast sedum grafting technology. In order to solve the problem of slow remediation speed caused by slow growth and small aboveground biomass of the southeast sedum, the rootstock reserved after the aboveground part of the southeast sedum is removed is used as a stock, and the new bud leaves of the feicao or the eight treasure sedum are used as scions, so that the grafted plants are formed through the grafting technology and are planted in the heavy metal contaminated soil, and the grafted plants utilize the root system of the southeast sedum to carry out phytoremediation on the heavy metals in the soil. After the grafted plants are planted in the contaminated soil, the Cd, Pb and Zn extraction amount of the southeast sedum can be improved, the extraction speed can be accelerated, and the remediation time can be shortened. In addition, the method is also helpful to improve the soil microbial diversity and stimulate more microbial groups participating in nutrient circulation and mutual symbiosis with plants. It can be seen through comprehensive comparison that the application can greatly shorten the plant extraction time, is helpful to soil health and has great application potential.

The invention discloses a disease prevention and growth promotion type compound microbial agent and application thereof to vegetables, and belongs to the technical field of microbial agents, and the compound microbial agent comprises burkholderia gladioli, bacillus cereus, antibiotic lysobacter and bacillus velezensis. The fertilizer provides nutrient substances and growth factors required by plants, promotes root growth, improves the soil environment and improves the nutrient absorption efficiency. By using natural microorganisms, the use of chemical pesticides and fertilizers is reduced, the environmental pollution is reduced, the diversity of soil microorganisms is improved, and the soil health is recovered. The biological agent disclosed by the invention can increase the yield and quality of crops, improve the economic benefit, improve the appearance and taste of fruits and improve the commodity value. The disease index of downy mildew is reduced by 34.14% compared with that of a control group, the disease index of powdery mildew is reduced by 25.53% compared with that of the control group, the wilt dead plant rate is reduced by 82.66% compared with that of the control group, the yield is increased by 12.37% compared with that of the control group, and the economic benefit is remarkable.

An organic fertilizer composition, referred to herein as fEx, derived from Tilapia biofloc sludge, consisting of essential micronutrients such as nitrogen (N), phosphorus (P2O5) and potassium (K2O), as well as micronutrients including calcium (Ca), iron (Fe), zinc (Zn), manganese (Mn), copper (Cu) and boron (B), and a high population of beneficial microorganisms, the fertilizer being formulated in both liquid and sludge form to promote plant growth, soil fertility and microbial diversity.

To provide a soil conditioner capable of improving variety and active value of microorganisms in a soil, a production method of the same, and a compost.SOLUTION: A soil conditioner of a fermentation composition is provided, including (A) a fermentation substrate, (B) refined molasses (C) water, and (D) ocean deep water. A compost has a soil conditioner of a fermentation composition including (A) a fermentation substrate, (B) refined molasses (C) water, and (D) ocean deep water, and one or two or more selected from the group consisting of (E) a bamboo powder, (F) an animal-derived dung and (G) rice bran. There is also provided, a production method of a soil conditioner which includes (A) a fermentation substrate, (B) refined molasses (C) water, and (D) ocean deep water, and which is fermented.SELECTED DRAWING: Figure 1

The invention relates to application of feather cockscomb and polyethylene micro-plastic in repairing tailing sand soil, and belongs to the technical field of biology. By adding the polyethylene micro-plastic into the soil and planting the feather cockscomb, the content of lead and cadmium in the soil can be effectively reduced, the physical and chemical properties of the soil are improved, the soil fertility is improved, the formation and stability of soil aggregates can be promoted, the erosion resistance of the soil is improved, and the microbial diversity in the soil is not damaged; and the purpose of repairing the tailing sand soil is achieved. In addition, by adding the micro-plastic into the soil, the accumulated biomass of the feather cockscomb can be increased, growth of the feather cockscomb is promoted, the repairing efficiency of the feather cockscomb on the tailing sand soil is further improved, a synergistic repairing effect is formed, and an ecological system achieves self-recovery and a virtuous circle.

The invention discloses a near-nano carbon-based material for function recovery and carbon fixation capability improvement of alien plant invasion damaged soil and a preparation method of the near-nano carbon-based material, and belongs to the technical field of soil remediation and carbon fixation and sink increase. The near-nano carbon-based material is prepared by the following steps: by taking an external invasive plant Canadian yellow flower stalk as a raw material, crushing, screening, mixing with water, carrying out hydrothermal reaction to obtain a hydrothermal carbonization product, and carrying out ball milling and screening treatment to obtain the near-nano carbon-based material. The obtained near-nano carbon-based material has a high-aromaticity carbon skeleton, a rich mesoporous-macroporous structure and a relatively high amorphous silicon content, can efficiently adsorb and passivate residual allelochemicals of solidago canadensis, reconstruct an aggregate structure, promote microbial diversity and key enzyme activity recovery, strengthen organic carbon-mineral bonding and improve the stability of the material. Therefore, invasion-induced multifunctional soil degradation is accurately reversed, and in-situ remediation of the soil structure and function and improvement of the carbon immobilization capacity are achieved. Experimental results show that the carbon-based material provided by the invention can increase the multifunctional index of invasion-induced damaged soil by about 560% and increase the carbon sequestration index by about 48.1%.

The invention discloses bacillus velezensis Bv-con2 and application of the bacillus velezensis Bv-con2 in turbot culture, and belongs to the technical field of microorganisms. The strain is preserved in the China Center for Type Culture Collection, the preservation address is Wuhan, China, the preservation date is December 27, 2024, and the preservation number is CCTCC NO: M 20242926. The bacterial strain has the beneficial effects that the bacterial strain has no pathogenicity to the turbot, has good probiotic potential, and is fermented to prepare a microbial inoculum which is mixed with the turbot to be added and fed to the turbot; the growth of the cultured scophthalmus maximus can be promoted, the trypsin and superoxide dismutase activity of the scophthalmus maximus can be improved, the intestinal flora structure of the scophthalmus maximus can be improved, and the intestinal microbial diversity level of the scophthalmus maximus can be improved; and the composition also can delay the morbidity and death of the scophthalmus maximus caused by infection of aeromonas salmonicida BHAS-1, edwardsiella salmonicida H4-S18, streptococcus parasuis BH2-4 and pseudomonas putida PPD2.

Compositions and methods for dietary fiber materials containing a plurality of distinct dietary fiber materials derived from distinct plant materials. At least some of the dietary fiber materials comprise fiber-bound polyphenols. Advantageously, the materials presented herein increase, upon oral administration, short chain fatty acid concentration, microbial biodiversity, and / or microbial abundance in the gut of the subject, even when compared to administration of a weight-equivalent dosage of inulin or psyllium.

The invention is applicable to the field of water and soil heavy metal pollution remediation, and provides a sulfur-modified carbon-based sulfate reducing microbial inoculum and application thereof in water and soil pollution remediation. In a preparation method, straw biochar is obtained by pretreating a straw raw material; and selecting one of a plurality of sulfur-modified straw carbon obtained based on sulfur modification treatment as a carrier, and loading sulfate reducing bacteria to obtain the sulfur-modified carbon-based sulfate reducing bacterial agent. The invention further provides application of the sulfur-modified carbon-based sulfate reducing bacterium agent in remediation of heavy metal pollution of water and soil. The sulfur-modified carbon-based sulfate reducing bacterium agent obtained by the invention has the capability of being stored at normal temperature, shows relatively high tolerance to heavy metals, and is an environment-friendly, low-cost and high-efficiency reducing bacterium product; the preparation method is simple in process, low in cost, suitable for remediation of polluted soil, capable of improving soil quality and maintaining microbial diversity and also suitable for treatment of polluted water.

The invention provides an acid soil conditioner and application thereof and an acid soil improvement method, and relates to the technical field of soil improvement, the acid soil conditioner comprises wormcast and a microbial agent; the microbial agent comprises penicillium kirschmannii with the viable bacterium concentration of 106 to 108 CFU / mL; the ratio of the mass of the wormcast to the volume of the microbial agent is (2-6g): (1-3mL). According to the acid soil conditioner provided by the invention, the wormcast and the penicillium kirschinii are applied in a compound manner, and a synergistic driving mechanism is constructed through organic nutrition input of the wormcast and functional microorganism guidance of the penicillium kirschinii, so that the pH, organic matter and nutrient content of soil can be improved, and a micro-ecological structure can be improved; the diversity and biological activity of soil microorganisms can be remarkably improved, the microbial network structure is optimized, the stability and toughness of a soil ecological system are enhanced, and the ecological function of acidified soil is restored, so that comprehensive improvement of crop biomass and nutrient utilization efficiency is promoted.

The application discloses a kind of composite functional microbial inoculant load clay mineral / submerged plant-based biological carbon sequestration materials and preparation method, by certain proportion submerged plant Vallisneria, Ceratophyllum, Potamogeton, clay mineral sepiolite powder, green detritus powder, kaolin powder, aerobic bacteria yeast, lactic acid bacteria, photosynthetic bacteria, denitrifying bacteria, anaerobic bacteria Bacillus, nitrifying bacteria, phosphorus-fixing bacteria, pseudomonas are constituted, steps are as follows: (1) submerged plant biochar precursor preparation;(2) clay mineral modification treatment;(3) carrier preparation;(4) bacteria culture;(5) microbial inoculant load: under natural conditions, microbial inoculant load, after natural air drying, obtain the material;It also relates to the use of the material, promote microbial activity, improve microbial diversity, effectively improve the bottom condition, provide favorable growth environment for the recovery and reconstruction of submerged plant, significantly promote the growth of submerged plant, further increase the carbon input of submerged plant, enhance the ecological carbon sink function of lake ecosystem.

The present invention relates to the technical field of desert sand-fixation and control, and provides a desertified soil restoration method combining shifting sand fixation and vegetation restoration. The desertified soil restoration method combining shifting sand fixation and vegetation restoration comprises the following steps: S1, vegetation selection, S2, sand-fixation tree grate manufacturing, S3, planting preparation, S4, planting, S5, growth condition monitoring, and S6, data sorting and analysis. Degradable tree grates are used for stabilizing the surface of shifting sand and preventing the surface of the shifting sand from moving; sand-fixation vegetation is then planted in the centers of the tree grates to stabilize shifting sand at deeper layers; a layered nutrient solution inside the degradable tree grates can slowly supply nutrients without affecting the use of pesticides; compost and cellulose produced after decomposition increase the activity of soil, improve the microbial diversity of the soil, and promote the activity of microorganisms in the soil, thereby enhancing the biological activity of the soil; and the cellulose decomposes in the soil, increasing the organic matter content of the soil, and improving the structure of the soil.

The invention discloses a new endophytic growth-promoting microbacterium species F1-18 of lavender and application of the new endophytic growth-promoting microbacterium species The invention provides an endophyte F1-18 separated from a Xinjiang Yili lavender seed. The endophyte F1-18 is a novel microbacterium sp. Strain with a plant growth promoting function, and the endophyte F1-18 is an endophyte F1-18 separated from the Xinjiang Yili lavender seed. The strain F1-18 has no hemolytic activity, has the capabilities of dissolving inorganic phosphorus, producing protease and secreting indoleacetic acid, and has strong tolerance to acid-base and high-salt environments. After the strain F1-18 is treated, the germination rate of lavender and corn seeds can be remarkably increased, the growth and development of lavender, corn and lettuce plants are promoted, the diversity of soil microorganisms is improved, and the microbial community structure is improved. The strain is expected to be developed into a novel agricultural microbial agent, and has important significance in reducing the use of chemical fertilizers, activating soil nutrients, improving the soil quality and improving the plant yield and quality.

This invention provides a soil conditioner, its preparation method, and its application, belonging to the field of soil remediation technology. The soil conditioner, by mass percentage, comprises the following components: 95%–97% tobacco straw, 0.5%–2% *Ulva prolifera* polysaccharide, 0.1%–0.5% fucoidan oligosaccharide, and 1%–2.5% *Bacillus* powder. Through the synergistic effect of its components, the soil conditioner provided by this invention can effectively improve soil compaction in coastal saline soils, enrich soil microbial biodiversity, and simultaneously enhance the salt and drought resistance of plants growing in coastal saline soils, thereby promoting plant growth and development. Example results show that the soil conditioner provided by this invention can significantly increase the stem height and dry weight of plants in coastal saline soils, increase the number of lateral branches, and promote root elongation; simultaneously, the soil conditioner can effectively improve the microbial diversity in coastal saline soils.

The invention provides a sphingomonas NT-1 and application thereof, the preservation number of the sphingomonas NT-1 is CCTCC NO: M 20252237, and the sphingomonas NT-1 is high in environmental adaptability and has multiple growth promoting functions of dissolving phosphorus, producing auxin and exopolysaccharide and the like. By applying the strain, the exfoliated substances in the mining area can be converted into an active matrix suitable for plant growth, the content of key nutrients such as nitrogen, phosphorus and potassium in the exfoliated substances and the microbial diversity are effectively improved, and resource reutilization of waste is achieved. In addition, the strain also significantly promotes the growth and nutrient accumulation of plants, and enhances the planting ability of vegetation. The invention provides an efficient and green microbial solution for in-situ utilization of the exfoliated substances in the mining area and rapid recovery of soil vegetation, and has important application value for promoting ecological restoration of mines and regeneration of land resources.