15 results about "Microbial biodiversity" patented technology

The invention relates to the technical field of agricultural cultivation, in particular to a farmland soil ecological structure restoration and soil fertility improvement cultivation method which comprises the steps that 1, the soil state is evaluated; 2, tillage depth is determined; step 3, adding organic materials; 4, tillage operation is carried out, a tillage machine is used for carrying out soil tillage, the tillage depth is the tillage depth determined in the step 2, and the tillage operation adopts a layered tillage mode including shallow tillage and deep tillage; step 5, inoculating a microbial agent, namely inoculating the soil microbial agent based on the soil microbial diversity and the soil enzyme activity in the step 1 after the cultivation operation; 6, covering and maintaining are conducted, after cultivation and inoculation, soil covering and maintaining are conducted, and using of covering materials and precise water and fertilizer management are included. Accurate soil evaluation and personalized tillage depth determination enable soil remediation to be more targeted and effective; organic materials and microorganisms are reasonably matched and used, so that the soil fertility and the biological activity are improved.

The invention relates to the technical field of bacterial fertilizers, in particular to a composite microbial fertilizer and a preparation method thereof. The composite microbial fertilizer is prepared from the following raw materials in parts by mass: 100 to 200 parts of sterilized organic manure, 50 to 150 parts of urea, 10 to 50 parts of ammonium humate, 50 to 70 parts of crop straw, 30 to 50 parts of hydroxyapatite, 80 to 200 parts of composite bamboo charcoal powder, 1 to 10 parts of a composite microbial agent, 10 to 20 parts of chitosan, 1 to 3 parts of polyethylene glycol and 40 to 120 parts of calcium chloride. The soil conditioner disclosed by the invention is beneficial to reducing the accumulation of soil salinity, reducing the salinization degree, enhancing the water and fertilizer retention performance of the soil, creating a good condition for the growth of crop roots, improving the soil structure, increasing the water retention and the air permeability, improving the microbial diversity and the fertility of the soil, and remarkably improving the yield and the quality of crops.

The utility model relates to the technical field of soil microorganism detection, and discloses a tobacco soil microorganism diversity detection device which comprises a detection box, the top of the detection box is fixedly connected with a treatment box, and the top of an inner cavity of the treatment box is provided with a stirring and scattering assembly. A screening assembly is arranged at the bottom of an inner cavity of the treatment box, and a reciprocating driving assembly is arranged on one side of the top of the detection box. After a power supply is switched on and a motor is started, the motor drives a rotating rod to rotate, so that a main bevel gear and an auxiliary bevel gear are linked, a stirring shaft and stirring blades rotate, sampled soil fed into a treatment box can be effectively scattered and stirred, meanwhile, a convex piece is driven to rotate to intermittently extrude a movable sleeve, and a reciprocating rod and a screening frame are driven to transversely shake; by means of the stirring and shaking combined mode, the soil can be stirred to the proper granularity, and the soil can be efficiently screened after falling into the screening frame.

The invention discloses a targeted biological bacterium coupling method for comprehensive treatment of coastal vegetable salt damage, heavy metal and pesticide residues and application, and relates to the field of agricultural biotechnology and soil remediation. According to the method, bacillus subtilis, bacillus mucilaginosus, brevibacillus laterosporus, pseudomonas fluorescens, arbuscular mycorrhizal fungi and host original flora extracted from healthy vegetable rhizosphere soil are subjected to coupled symbiotic culture, and target flora with multiple functions is formed. The functional flora is compounded with a nutrient carrier containing potassium humate and fulvic acid, a seaweed extract and a specific salt-resistant additive to prepare the multifunctional water-soluble fertilizer or fungicide. After the compound fertilizer is applied to a coastal vegetable production base, the synergistic effects of degrading soil pesticide residues, passivating heavy metals, enhancing the salt tolerance of vegetables and improving the soil fertility and microbial diversity can be synchronously realized, the compound obstacle problem of coastal vegetable production is fundamentally solved, and the yield, quality and safety of vegetables are guaranteed.

The invention relates to a soil conditioner for improving facility vegetable field secondary salinization and a preparation method and application thereof, and belongs to the technical field of facility agriculture soil improvement, the soil conditioner for improving facility vegetable field secondary salinization comprises mushroom dregs, cow dung, humic acid, a microbial agent and a microbial protective agent; the microbial agent is prepared from brevibacillus laterosporus, bacillus megaterium and bacillus subtilis; the microbial protective agent comprises the following components: alginate oligosaccharide, vegetable oil, ectoine and fumaric acid. The modifier provided by the invention is beneficial to improving soil organic matters, improving the utilization rate of soil nutrients, preventing soil hardening, improving the diversity of soil microorganisms and reducing the influence of a salt stress environment on vegetable growth.

The application discloses a heavy metal contaminated soil remediation method based on a southeast sedum grafting technology. In order to solve the problem of slow remediation speed caused by slow growth and small aboveground biomass of the southeast sedum, the rootstock reserved after the aboveground part of the southeast sedum is removed is used as a stock, and the new bud leaves of the feicao or the eight treasure sedum are used as scions, so that the grafted plants are formed through the grafting technology and are planted in the heavy metal contaminated soil, and the grafted plants utilize the root system of the southeast sedum to carry out phytoremediation on the heavy metals in the soil. After the grafted plants are planted in the contaminated soil, the Cd, Pb and Zn extraction amount of the southeast sedum can be improved, the extraction speed can be accelerated, and the remediation time can be shortened. In addition, the method is also helpful to improve the soil microbial diversity and stimulate more microbial groups participating in nutrient circulation and mutual symbiosis with plants. It can be seen through comprehensive comparison that the application can greatly shorten the plant extraction time, is helpful to soil health and has great application potential.

An organic fertilizer composition, referred to herein as fEx, derived from Tilapia biofloc sludge, consisting of essential micronutrients such as nitrogen (N), phosphorus (P2O5) and potassium (K2O), as well as micronutrients including calcium (Ca), iron (Fe), zinc (Zn), manganese (Mn), copper (Cu) and boron (B), and a high population of beneficial microorganisms, the fertilizer being formulated in both liquid and sludge form to promote plant growth, soil fertility and microbial diversity.

The invention discloses a near-nano carbon-based material for function recovery and carbon fixation capability improvement of alien plant invasion damaged soil and a preparation method of the near-nano carbon-based material, and belongs to the technical field of soil remediation and carbon fixation and sink increase. The near-nano carbon-based material is prepared by the following steps: by taking an external invasive plant Canadian yellow flower stalk as a raw material, crushing, screening, mixing with water, carrying out hydrothermal reaction to obtain a hydrothermal carbonization product, and carrying out ball milling and screening treatment to obtain the near-nano carbon-based material. The obtained near-nano carbon-based material has a high-aromaticity carbon skeleton, a rich mesoporous-macroporous structure and a relatively high amorphous silicon content, can efficiently adsorb and passivate residual allelochemicals of solidago canadensis, reconstruct an aggregate structure, promote microbial diversity and key enzyme activity recovery, strengthen organic carbon-mineral bonding and improve the stability of the material. Therefore, invasion-induced multifunctional soil degradation is accurately reversed, and in-situ remediation of the soil structure and function and improvement of the carbon immobilization capacity are achieved. Experimental results show that the carbon-based material provided by the invention can increase the multifunctional index of invasion-induced damaged soil by about 560% and increase the carbon sequestration index by about 48.1%.

The invention provides an acid soil conditioner and application thereof and an acid soil improvement method, and relates to the technical field of soil improvement, the acid soil conditioner comprises wormcast and a microbial agent; the microbial agent comprises penicillium kirschmannii with the viable bacterium concentration of 106 to 108 CFU / mL; the ratio of the mass of the wormcast to the volume of the microbial agent is (2-6g): (1-3mL). According to the acid soil conditioner provided by the invention, the wormcast and the penicillium kirschinii are applied in a compound manner, and a synergistic driving mechanism is constructed through organic nutrition input of the wormcast and functional microorganism guidance of the penicillium kirschinii, so that the pH, organic matter and nutrient content of soil can be improved, and a micro-ecological structure can be improved; the diversity and biological activity of soil microorganisms can be remarkably improved, the microbial network structure is optimized, the stability and toughness of a soil ecological system are enhanced, and the ecological function of acidified soil is restored, so that comprehensive improvement of crop biomass and nutrient utilization efficiency is promoted.

The application discloses a kind of composite functional microbial inoculant load clay mineral / submerged plant-based biological carbon sequestration materials and preparation method, by certain proportion submerged plant Vallisneria, Ceratophyllum, Potamogeton, clay mineral sepiolite powder, green detritus powder, kaolin powder, aerobic bacteria yeast, lactic acid bacteria, photosynthetic bacteria, denitrifying bacteria, anaerobic bacteria Bacillus, nitrifying bacteria, phosphorus-fixing bacteria, pseudomonas are constituted, steps are as follows: (1) submerged plant biochar precursor preparation;(2) clay mineral modification treatment;(3) carrier preparation;(4) bacteria culture;(5) microbial inoculant load: under natural conditions, microbial inoculant load, after natural air drying, obtain the material;It also relates to the use of the material, promote microbial activity, improve microbial diversity, effectively improve the bottom condition, provide favorable growth environment for the recovery and reconstruction of submerged plant, significantly promote the growth of submerged plant, further increase the carbon input of submerged plant, enhance the ecological carbon sink function of lake ecosystem.

The present invention relates to the technical field of desert sand-fixation and control, and provides a desertified soil restoration method combining shifting sand fixation and vegetation restoration. The desertified soil restoration method combining shifting sand fixation and vegetation restoration comprises the following steps: S1, vegetation selection, S2, sand-fixation tree grate manufacturing, S3, planting preparation, S4, planting, S5, growth condition monitoring, and S6, data sorting and analysis. Degradable tree grates are used for stabilizing the surface of shifting sand and preventing the surface of the shifting sand from moving; sand-fixation vegetation is then planted in the centers of the tree grates to stabilize shifting sand at deeper layers; a layered nutrient solution inside the degradable tree grates can slowly supply nutrients without affecting the use of pesticides; compost and cellulose produced after decomposition increase the activity of soil, improve the microbial diversity of the soil, and promote the activity of microorganisms in the soil, thereby enhancing the biological activity of the soil; and the cellulose decomposes in the soil, increasing the organic matter content of the soil, and improving the structure of the soil.

The invention discloses a new endophytic growth-promoting microbacterium species F1-18 of lavender and application of the new endophytic growth-promoting microbacterium species The invention provides an endophyte F1-18 separated from a Xinjiang Yili lavender seed. The endophyte F1-18 is a novel microbacterium sp. Strain with a plant growth promoting function, and the endophyte F1-18 is an endophyte F1-18 separated from the Xinjiang Yili lavender seed. The strain F1-18 has no hemolytic activity, has the capabilities of dissolving inorganic phosphorus, producing protease and secreting indoleacetic acid, and has strong tolerance to acid-base and high-salt environments. After the strain F1-18 is treated, the germination rate of lavender and corn seeds can be remarkably increased, the growth and development of lavender, corn and lettuce plants are promoted, the diversity of soil microorganisms is improved, and the microbial community structure is improved. The strain is expected to be developed into a novel agricultural microbial agent, and has important significance in reducing the use of chemical fertilizers, activating soil nutrients, improving the soil quality and improving the plant yield and quality.

This invention provides a soil conditioner, its preparation method, and its application, belonging to the field of soil remediation technology. The soil conditioner, by mass percentage, comprises the following components: 95%–97% tobacco straw, 0.5%–2% *Ulva prolifera* polysaccharide, 0.1%–0.5% fucoidan oligosaccharide, and 1%–2.5% *Bacillus* powder. Through the synergistic effect of its components, the soil conditioner provided by this invention can effectively improve soil compaction in coastal saline soils, enrich soil microbial biodiversity, and simultaneously enhance the salt and drought resistance of plants growing in coastal saline soils, thereby promoting plant growth and development. Example results show that the soil conditioner provided by this invention can significantly increase the stem height and dry weight of plants in coastal saline soils, increase the number of lateral branches, and promote root elongation; simultaneously, the soil conditioner can effectively improve the microbial diversity in coastal saline soils.

The invention provides a sphingomonas NT-1 and application thereof, the preservation number of the sphingomonas NT-1 is CCTCC NO: M 20252237, and the sphingomonas NT-1 is high in environmental adaptability and has multiple growth promoting functions of dissolving phosphorus, producing auxin and exopolysaccharide and the like. By applying the strain, the exfoliated substances in the mining area can be converted into an active matrix suitable for plant growth, the content of key nutrients such as nitrogen, phosphorus and potassium in the exfoliated substances and the microbial diversity are effectively improved, and resource reutilization of waste is achieved. In addition, the strain also significantly promotes the growth and nutrient accumulation of plants, and enhances the planting ability of vegetation. The invention provides an efficient and green microbial solution for in-situ utilization of the exfoliated substances in the mining area and rapid recovery of soil vegetation, and has important application value for promoting ecological restoration of mines and regeneration of land resources.

The application relates to the technical field of agricultural intelligent fertilization, and discloses a fertilization decision-making method and system based on soil microbial diversity analysis, which comprises the following steps: obtaining soil samples, environmental parameters and crop growth data, analyzing the soil samples to obtain microbial community composition data and soil physicochemical parameters; determining the functional structure characteristics of the microbial community based on the microbial community composition data; generating soil parameter distribution characteristics based on the soil physicochemical parameters; performing weighted fusion on the functional structure characteristics and the soil parameter distribution characteristics based on a double-flow graph convolution-attention model to obtain soil microbial fusion characteristics; generating the microbial metabolic rate and the crop nutrient demand increment based on the functional structure characteristics of the microbial community, the environmental parameters, the soil parameter distribution characteristics and the crop growth data; and inputting the microbial metabolic rate, the crop nutrient demand increment, the fusion characteristics, the environmental parameters and the soil parameter distribution characteristics into a reinforcement learning model to obtain a target fertilization decision.