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Bile salt tolerant and cholesterol-reducing probiotic composition and application thereof

A technology for lowering cholesterol and probiotics, applied in the field of probiotics, can solve the problem that the functional application of combined strains is rarely studied, etc.

Inactive Publication Date: 2021-07-27
ALAND BIOTECHNOLOGY RES TAIZHOU CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the functional application of combined strains is rarely studied

Method used

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  • Bile salt tolerant and cholesterol-reducing probiotic composition and application thereof
  • Bile salt tolerant and cholesterol-reducing probiotic composition and application thereof
  • Bile salt tolerant and cholesterol-reducing probiotic composition and application thereof

Examples

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preparation example Construction

[0027] The preparation method of the probiotic composition is not particularly limited in the present invention, preferably the Lactobacillus paracasei and Lactobacillus acidophilus are co-inoculated on the MRS broth medium according to the volume ratio of 1:1 for anaerobic culture (co-cultivate).

[0028] The present invention also provides the application of the above-mentioned probiotic composition in improving the enzyme activity of bile salt hydrolyzing enzyme.

[0029] In the embodiment of the present invention, the bile salt hydrolysis enzyme activity is represented by the opaque halo feature produced by the colony, and the probiotic composition contains 0.2% (w / v) taurodeoxycholic acid sodium salt and 0.37g / L chloride When growing on the calcium-calcified MRS agar, a more obvious opaque halo was produced, which was more obvious than that produced by any single bacterial strain, confirming that the bile salt hydrolyzing enzyme activity of the probiotic composition was h...

Embodiment 1

[0037] Screening and Identification of Strains

[0038] Selective medium (Bifidobacterium: TPY agar; Lactobacillus: modified MC agar) was used to isolate the strains from the intestines of healthy young people and children. gram, placed in a sterile collection tube, added 50ml of sterile water within 1 hour, and oscillated to make a homogenate. Serially dilute 10-fold concentration with sterile water to 10 -5;Take 0.1ml of samples with different dilutions and spread them on the two selective media, and incubate anaerobically at 37°C for 48 hours; take out the petri dish, pick single colonies with different morphological characteristics, and transfer them to liquid MRS broth medium, 37 After 24 hours of anaerobic culture at ℃, send it to the sequencing company to detect and identify the strains, and the identified strains can be frozen for preservation after three consecutive passages.

[0039] The single colony of Lactobacillus paracasei AI-62 obtained by screening was white...

Embodiment 2

[0042] Safety Evaluation of Strains

[0043] The glycerol tube strain (co-culture strain, expressed as: Lactobacillus paracasei AI-62*Lactobacillus acidophilus AI-32) obtained in Example 1, which was frozen at -20°C, was inoculated and recovered in normal MRS broth medium , and carried out 3 transfer activation cultures (anaerobic culture at 37° C. for 24 h), and the activated culture medium was used for antibiotic susceptibility experiments.

[0044] Take 0.5mL of the activated Lactobacillus paracasei AI-62 and Lactobacillus acidophilus AI-32 respectively, spread evenly on the surface of the MRS agar plate, and after drying, add gentamicin, vancomycin, kanamycin Paste the drug-sensitive paper sheets of clindamycin, streptomycin, ampicillin, penicillin, erythromycin, and chloramphenicol on the surface of the agar, place them in an incubator at 37°C for 24 hours, and observe the growth of the strains on the agar surface. If an obvious transparent circle appears around the anti...

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Abstract

The invention provides a bile salt tolerant and cholesterol-reducing probiotic composition and application thereof, and relates to the technical field of probiotics. The probiotic composition disclosed by the invention comprises lactobacillus paracasei and lactobacillus acidophilus. In the embodiment of the invention, the lactobacillus paracasei AI-62 and the lactobacillus acidophilus AI-32 are used for co-culture, so that the bile salt tolerance and the cholesterol utilization rate which are obviously superior to those of a single strain are achieved, and the activity of bile salt hydrolase can be remarkably improved; the probiotic composition can be applied to foods, functional foods and dietary supplements so as to reduce the risks of hyperlipidemia, atherosclerosis and the like caused by serum cholesterol and high cholesterol.

Description

technical field [0001] The invention belongs to the technical field of probiotics, and in particular relates to a bile salt-tolerant and cholesterol-lowering probiotic composition and an application thereof. Background technique [0002] Probiotics exist widely in nature, mainly from human and animal intestines, mainly including Lactobacillus and Bifidobacteria; probiotics are beneficial to the host by colonizing in the human body and changing the composition of the flora in a certain part of the host active microorganisms. At present, probiotics are widely used in the field of food processing and related health products. It is recognized that the intake of a certain amount of live probiotic products has the functions of intestinal health, promoting intestinal absorption, enhancing human immunity and alleviating allergic reactions. [0003] In recent years, people have gradually isolated probiotic strains from the human body, from the location of probiotic strains to the st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/80A23L33/135A23L33/10C12R1/225C12R1/23
CPCC12N1/20C12N9/80C12Y305/01024A23L33/135A23L33/10A23V2002/00A23V2400/113A23V2400/165A23V2200/326A23V2200/3262A23V2200/332
Inventor 李雪君张国栋赵宇徐成炜
Owner ALAND BIOTECHNOLOGY RES TAIZHOU CO LTD
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