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Method for detecting content of pirimicarb in food by combining sulfur-doped carbon quantum dots with enzyme inhibition method

A technology of carbon quantum dots and anti-pirimicarb, which is applied in the directions of measuring devices, material excitation analysis, fluorescence/phosphorescence, etc., to achieve the effects of fast detection speed, less extraction agent consumption, and reduced fluorescence intensity

Active Publication Date: 2021-07-30
SHANXI AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the results of the above detection methods are sensitive and accurate, there is still room for improvement in sample extraction, sample detection, etc.

Method used

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  • Method for detecting content of pirimicarb in food by combining sulfur-doped carbon quantum dots with enzyme inhibition method
  • Method for detecting content of pirimicarb in food by combining sulfur-doped carbon quantum dots with enzyme inhibition method
  • Method for detecting content of pirimicarb in food by combining sulfur-doped carbon quantum dots with enzyme inhibition method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1, the synthesis of sulfur-doped carbon quantum dots

[0046]Weigh 0.400g of citric acid and dissolve it in 40mL of deionized water, add 0.113g of sodium sulfide after dissolving, stir and dissolve, place in a 50mL autoclave, heat and react at high temperature (190°C) for 12h to synthesize sulfur-doped carbon quantum dots (S -CDQs), determine the emission wavelength of its optimal excitation wavelength.

[0047] The fluorescence spectrum of the sulfur-doped carbon quantum dots prepared in this example is as follows figure 2 shown.

[0048] Depend on figure 2 It can be seen that the optimum excitation wavelength of the S-CDQs synthesized in the present invention is 325nm, and the optimum emission wavelength is 395nm.

Embodiment 2

[0049] Embodiment 2, feasibility verification of test

[0050] In order to verify the feasibility of the detection method of the present invention, two different reaction systems were prepared, a: acetylcholinesterase + acetylthiocholine iodide + buffer (pH=7) + S-CQDs, b: pirimicarb + acetylcholinesterase + acetylthiocholine iodide + buffer solution (pH = 7) + S-CQDs, measure the fluorescence spectrum at the optimal excitation wavelength. Among them, in system a, add 100 μL of methanol solution to a 96-well plate, then add 50 μL of acetylcholinesterase at 50 mU / mL, and incubate at a constant temperature of 37° C. for 50 min. After that, 50 μL of 2 mmol / L acetylthiocholine iodide, 50 μL of pH=7 buffer solution and 50 μL of sulfur-doped carbon quantum dots were added, incubated at 37° C. for 40 min, and the fluorescence value was measured. In system b, add 100 μL of pirimicarb at a concentration of 200 μmol / L to a 96-well plate, then add 50 μL of acetylcholinesterase at 2 mU / m...

Embodiment 3

[0053] Example 3. Optimization of conditions for detection of pirimicarb by sulfur-doped carbon quantum dots combined with enzyme inhibition method

[0054] A. Water-soluble DES components

[0055] Prepare three different water-soluble DES (volume greater than 1mL), a: choline chloride + ethylene glycol; b: choline chloride + propylene glycol; c: choline chloride + butanediol; And ethylene / propylene / butanediol in a molar ratio of 1:1 and heated until there are no obvious particles and become transparent. Weigh 1 g of crushed blank sample (rice) into a 10 mL centrifuge tube, add 50 μL of 150 μmol / L pirimicarb, and let stand for 20 minutes. Add different synthetic DES (volume 1mL) and vortex extract for 15s under the condition of 2500r / min. After the extraction was completed, 100 μL of the extract was added to a 96-well plate, 50 μL of 50 mU / mL acetylcholinesterase was added, and incubated at 37° C. for 50 min to fully inhibit the activity of the enzyme. Afterwards, 50 μL of ...

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Abstract

The invention discloses a method for detecting the content of pirimicarb in food by combining sulfur-doped carbon quantum dots with an enzyme inhibition method. The method comprises the following steps: S1, preparing pirimicarb standard solutions with different concentrations, and respectively adding the pirimicarb standard solutions into a crushed blank food sample; extracting by using water-soluble DES, adding acetylcholin esterase into an extracting solution, culturing, adding acetylthiocholine iodide, a pH buffer solution and S-CQDs, and culturing at constant temperature again; measuring fluorescence values of solutions with different concentrations, and drawing a standard curve; s2, extracting the crushed food to be detected by adopting water-soluble DES; s3, taking the extracting solution obtained in the step S2, and operating according to the step S1 to obtain the fluorescence intensity of the system; obtaining the fluorescence according to a standard curve and the measured fluorescence intensity. The extracting solution of the water-soluble DES is mutually soluble with a subsequent detection system, and the surface of the S-CQDs contains a large amount of negative charges which can generate electrostatic interaction with a hydrolysate thiocholine (+) of acetylthiocholine to cause fluorescence quenching; pirimicarb can inhibit enzyme activity, system fluorescence is enhanced, and quantitative detection is achieved.

Description

technical field [0001] The invention relates to a method for detecting the content of pirimicarb in food by combining sulfur-doped carbon quantum dots with an enzyme inhibition method, belonging to the field of food safety detection. Background technique [0002] Crop pest and disease prevention is an important aspect of agricultural management. In recent years, many insecticides have been developed to control pests and greatly increase the overall yield of crops. Pirimicarb has the functions of contact killing, fumigation and foliar penetration. It is an efficient and selective carbamate aphidicide. It is suitable for controlling aphids on vegetables, tobacco and food crops. It is an ideal agent for comprehensive control. It has been widely and a large number of applications in grain and other vegetable cultivation. However, the irrational use of pirimicarb and other pesticides has also caused food safety problems. Carbamate pesticides are inhibitors of mammalian acetylc...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6402
Inventor 荆旭王慧慧郭璐瑶王晓闻王愈冯翠萍李晋栋
Owner SHANXI AGRI UNIV
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