Injectable silk fibroin/nano-hydroxyapatite composite hydrogel and preparation method thereof

Abstract

The invention provides injectable silk fibroin / nano-hydroxyapatite composite hydrogel and a preparation method thereof. The preparation method comprises the following steps that silkworm cocoons are degummed and dried to obtain silk fibroin, the silk fibroin is dissolved with a solvent at a certain temperature and then mixed with phosphate to obtain a mixed solution, the pH value of the mixed solution is adjusted, and after reaction is conducted for a period of time, performing dialyzing and drying to obtain silk fibroin / nano-hydroxyapatite powder, and then performing ultrasonic treatment to generate the silk fibroin / nano-hydroxyapatite composite hydrogel. The mass volume concentration of the silk fibroin / hydroxyapatite powder is 0.1%-90%; The mass ratio of the silk fibroin to the hydroxyapatite is (5-99): (1-95). The prepared composite hydrogel is uniform, good in interface bonding, free of bubbles inside, safe, non-toxic and excellent in injectability. The method is simple in preparation process, mild in condition, free of chemical cross-linking agents in the whole process, environmentally friendly, low in cost and easy to industrialize, popularize and apply.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to an injectable silk fibroin / nanometer hydroxyapatite composite hydrogel and a preparation method thereof. Background technique [0002] Hydrogel is a kind of cross-linked polymer with a three-dimensional network structure, which can well simulate the water environment of the extracellular matrix, and provide a microenvironment similar to the natural extracellular matrix for cell adhesion, proliferation and differentiation. It is an ideal material for repair and reconstruction, so it is widely used in biomedical fields, such as medical dressings, cartilage tissue repair, cell transplantation, drug delivery and tissue engineering scaffolds, etc., especially injectable hydrogels for tissue repair. Attractive, it can not only be transplanted to the defect site in a minimally invasive manner, but also can fill defects of any shape, and at the same time facilitate the loadi...

AI Technical Summary

Problems solved by technology

However, the above method is essentially physical mixing, not a combination of chemical bonds, and its interface bonding strength, mixing scale and uniformity are difficult to guarantee

In addition, the regenerated silk fibroin solution is extremely unstable, and even if it is stored at low temperature, it will spontaneously form a gel over time. The preparation, transportation and preservation of the drug have brought great inconvenience, and the preparation process is very complicated and cumbersome, thus limiting its clinical application and effectiveness.

Because hydroxyapatite has good biocompatibility and osteoinductivity, it is often used as a material for injectable hydrogels. However, hydrogel products prepared from hydroxyapatite are currently facing many problems: hydroxyapatite Stone powder is prone to agglomeration due to its small particle size, resulting in uneven distribution of inorganic phases in composite materials, difficulty in removing air bubbles, and difficulty in storage and transportation of hydrogel raw materials.

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