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Preparation method of plant essential oil capable of inhibiting tyrosinase activity

A technology of plant essential oil and tyrosinase, applied in the field of plant essential oil, can solve the problem of weak tyrosinase inhibitory activity, etc., and achieve the effects of widening the scope of use, convenient operation and inhibiting tyrosinase activity

Pending Publication Date: 2021-08-06
WUYI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the tyrosinase inhibitory activity of rose oil and lemon oil is not strong

Method used

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  • Preparation method of plant essential oil capable of inhibiting tyrosinase activity
  • Preparation method of plant essential oil capable of inhibiting tyrosinase activity
  • Preparation method of plant essential oil capable of inhibiting tyrosinase activity

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Experimental program
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Effect test

Embodiment 1

[0035] The present embodiment provides a method for preparing plant essential oils that inhibit tyrosinase activity. The method uses supercritical CO 2 Extraction method, comprising the following steps:

[0036] S11), take by weighing dry plant powder 100g in the supercritical extraction kettle, CO 2 Inject the extraction kettle under high pressure; and measure the CO with a carbon dioxide flow meter 2 Dosage, and in the supercritical extraction process, in order to ensure the maximum extraction rate, the extraction temperature and pressure are controlled by the regulating valve. Among them, the flow rate of carbon dioxide is 5-40L / h, the extraction temperature is 30-50°C, the extraction pressure is 10-30Mpa, and the extraction time is 60-120min;

[0037] S21), after the extraction is completed, adjust the rotational speed of the rotary vacuum evaporation instrument to 60rpm / min, the vacuum degree to 0.2MPa, the evaporation temperature to 42°C, and the distillation time to 4...

Embodiment 2

[0040] The present embodiment provides a method for preparing plant essential oils with tyrosinase-inhibiting activity, the method adopts ethanol extraction method, comprising the following steps:

[0041] S201), weighing 100 g of dried plant powder into an extractor, adding ethanol, refluxing and extracting, filtering, combining extracts, and concentrating under reduced pressure to obtain a concentrate. The number of reflux extractions is 1-5 times, the temperature of reflux extraction is 40-120°C, and the time of each reflux extraction is 30-80 minutes;

[0042] S202), after the extraction is completed, adjust the rotational speed of the rotary vacuum evaporation instrument to be 60rpm / min, the vacuum degree to be 0.2MPa, the evaporation temperature to be 40°C, and the distillation time to be 40min;

[0043] S203), weighing the collected volatile oil, and calculating the extraction rate, at this time, the extraction rate of the extracted plant essential oil is 2.0-6.0%.

Embodiment 3

[0045] In vitro experiment of essential oil composition inhibiting tyrosinase activity

[0046] First, prepare tyrosinase solution with 0.05mol / L phosphate buffer solution, first add 0.05mol / L phosphate buffer solution to each well of the 96-well plate, the amount added is 85μL, and the purpose of adding PBS solution is to react Create a suitable environment, then add 10 μL of tyrosinase solution, and then add 5 μL of essential oil sample solution (choose DMSO or acetone to dissolve according to the solubility of essential oil), and use 5 μL DMSO or acetone solution instead of sample solution for the negative control group. Wells were repeated 4 times. Then the 96-well plate was quickly put in and shaken for 30s, and then incubated at 37°C for 9min30s.

[0047] Next, take out the plate after the incubation is completed, add 100 μL of L-DOPA solution with a concentration of 2 mmol / L to initiate the reaction, and quickly put the plate back into the microplate reader, measure th...

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Abstract

The invention provides a preparation method of plant essential oil capable of inhibiting tyrosinase activity, the preparation method comprises a supercritical CO2 extraction method and an ethanol extraction method, the supercritical CO2 extraction method has a carbon dioxide flow rate of 5-40 L / h, an extraction temperature of 30-50 DEG C, an extraction pressure of 10-30 Mpa, an extraction time of 60-120 min and an extraction rate of 3.5%-6.0%; the reflux extraction frequency of the ethanol extraction method is 1-5 times, the reflux extraction temperature is 40-120 DEG C, the reflux extraction time of each time is 30-80 minutes, and the extraction rate is 2.0%-6.0%. The plant essential oil prepared by the invention can obviously inhibit tyrosinase in vitro. The plant essential oil obtained through supercritical CO2 extraction can play a role in inhibiting tyrosinase. Ganoderma lucidum spore oil, ligusticum chuanxiong hort oil, rose oil, cinnamon essential oil, mangnolia officinalis oil, folium artemisiae argyi essential oil, carnation oil, green tea seed essential oil and laurel essential oil are adopted, and the inhibition effect on tyrosinase is good.

Description

technical field [0001] The invention relates to the technical field of plant essential oils, in particular to a method for preparing plant essential oils with tyrosinase-inhibiting activity. Background technique [0002] Tyrosinase, also known as polyphenol oxidase, is a multifunctional copper-containing oxidoreductase that is ubiquitous in mammals, plants, microorganisms and insects. Under the action of tyrosinase, tyrosine is transformed into L-dopamine (3,4-dihydroxyphenylalanine), and dopamine is transformed into dopaquinone, which, after a series of reactions, accumulates and aggregates into melanin . Although the appearance of melanin in human skin is mostly due to the body's response measures to protect against ultraviolet rays, excessive accumulation of melanin can cause a series of skin problems, such as: melasma, sunburn, and moles. The amount of melanin produced is directly proportional to the expression and activity of tyrosinase. The lower the activity of tyro...

Claims

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Application Information

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IPC IPC(8): C11B9/02
CPCC11B9/025C11B9/022
Inventor 刘文锋游宗霖曹樱凡
Owner WUYI UNIV
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