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Sodium nitrite-gelatin microsphere and preparation method and application thereof

A technology of sodium nitrite and gelatin, which is applied in the direction of application, preservation of meat/fish with chemicals, preservation of meat/fish with coating protection layer, etc., to achieve the effect of stable properties, simple operation and low price

Pending Publication Date: 2021-08-13
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no mature technology to convert NaNO 2 Embedded in microspheres for long-term sustained release

Method used

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  • Sodium nitrite-gelatin microsphere and preparation method and application thereof
  • Sodium nitrite-gelatin microsphere and preparation method and application thereof
  • Sodium nitrite-gelatin microsphere and preparation method and application thereof

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preparation example Construction

[0051] The embodiment of the present invention discloses a preparation method of sodium nitrite-gelatin microspheres, specifically comprising the following steps:

[0052] (1) Add gelatin to deionized water and stir until completely dissolved to obtain a gelatin solution with a mass concentration of 0.01-0.1g / mL; wherein, the gelatin includes type A gelatin or type B gelatin; and a water bath at 50-55°C is used during the stirring process Heating by means of heating, the stirring speed is 100-500r / min;

[0053] (2) Then sodium nitrite is added to the gelatin solution according to the mass ratio of 1: (5-15) to the gelatin, and the stirring is continued until it is completely dissolved to obtain a mixed solution; wherein the temperature during the stirring process is 15-30°C , the stirring speed is 100-500r / min;

[0054] (3) Add transglutaminase (TGase) with a volume of 1-5mL and an enzyme activity of 120U / mL into the above mixed solution, and then stir and react to obtain a c...

Embodiment 1～5

[0060] Embodiments 1 to 5 of the present invention disclose a preparation method of sodium nitrite-gelatin microspheres, specifically comprising the following steps:

[0061] (1) Add 1g of type A gelatin powder into 20mL of deionized water, and then stir it with a magnetic stirrer until completely dissolved in a water bath at 50-55°C to obtain a gelatin solution with a mass concentration of 0.05g / mL; 200r / min;

[0062] (2) Then 100mg of NaNO 2 Add it to the above gelatin solution, and stir until it is completely dissolved to obtain a mixed solution; the temperature during the stirring process is 25°C, and the stirring speed is 200r / min;

[0063] (3) Add transglutaminase (TGase) with an enzyme activity of 120U / mL to the above mixed solution according to the concentration of 0U / g, 10U / g, 20U / g, 30U / g, and 40U / g respectively, and then stir the reaction 2h obtains cross-linking solution;

[0064] (4) Add the emulsifier compounded by 2mL Span 80 and 1mL Tween 80 to 160mL corn oi...

Embodiment 6～10

[0068] Embodiments 6-10 of the present invention disclose a preparation method of sodium nitrite-gelatin microspheres, specifically comprising the following steps:

[0069] (1) Add 1g of B-type gelatin powder to 20mL of deionized water, and then stir it with a magnetic stirrer until it is completely dissolved in a water bath at 50-55°C to obtain a gelatin solution with a mass concentration of 0.05g / mL; 200r / min;

[0070] (2) Then 100mg of NaNO 2 Add it to the above gelatin solution, and stir until it is completely dissolved to obtain a mixed solution; the temperature during the stirring process is 25°C, and the stirring speed is 200r / min;

[0071] (3) Add transglutaminase (TGase) with an enzyme activity of 120U / mL to the above mixed solution according to the concentration of 0U / g, 10U / g, 20U / g, 30U / g, and 40U / g respectively, and then stir the reaction 2h obtains cross-linking solution;

[0072] (4) Add the emulsifier compounded by 2mL Span 80 and 1mL Tween 80 into 160mL cor...

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Abstract

The invention discloses a sodium nitrite-gelatin microsphere and a preparation method and application thereof. The preparation method comprises the following steps of: firstly, adding gelatin into deionized water, and stirring until the gelatin is completely dissolved to obtain a gelatin solution; then adding sodium nitrite into the gelatin solution, and continuously stirring until the sodium nitrite is completely dissolved to obtain a mixed solution; then adding glutamine transaminase into the mixed solution, and then stirring for reaction to obtain a cross-linked solution; then adding an emulsifier into an oil phase, then dropwise adding the cross-linked solution under the stirring condition, and continuously carrying out emulsification reaction under the stirring condition to form an emulsion; and finally, cooling the emulsion under the stirring condition, and carrying out operations such as ultrasonic treatment, suction filtration separation and washing to finally obtain the sodium nitrite-gelatin microsphere. The preparation method is simple in overall operation, and the prepared sodium nitrite-gelatin microsphere is smooth in surface and uniform in particle size, has the average particle size of 5-40 microns, can slowly release NO in a weak acidic environment, and can be applied to food antibacterial agents.

Description

technical field [0001] The invention relates to the technical field of polymer materials, and more specifically relates to a sodium nitrite-gelatin microsphere and a preparation method and application thereof. Background technique [0002] Currently, sodium nitrite (NaNO 2 ) as one of the most commonly used coloring agents and antibacterial agents is widely used in meat processing. Related studies have shown that NaNO 2 Can produce NO radicals (NO·), nitrogen dioxide (NO 2 ), Dinitrogen trioxide (N 2 o 3 ) and peroxynitrite (ONOO·) and other active substances, these active substances induce bacterial DNA strand breaks through free radicals, promote lipid peroxidation, and then kill bacteria. In addition, in the weakly acidic environment produced by microorganisms, NaNO 2 Produce NO, which plays a vital role in antibacterial, and can effectively kill not only single strains, but also mixed microbial strains. [0003] However, when NaNO 2 When applied externally to foo...

Claims

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Application Information

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IPC IPC(8): A23B4/10A23B4/24A23P10/30
CPCA23B4/10A23B4/24A23P10/30A23V2002/00A23V2200/10A23V2200/222A23V2250/5432
Inventor 田晓静王稳航孙孟娇师小婷
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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